A method for directly extracting fatty acid methyl esters from microalgae wet biomass
A fatty acid methyl ester and biomass technology, applied in the direction of fatty acid esterification, fat production, fatty acid production, etc., can solve the problem of high product cost, achieve scientific design, reasonable process, and improve simplicity
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Embodiment 1
[0029] Centrifuge at 4000rpm for 10min to collect the cell density to 10 7 100 mL of Phaeodactylum tricornutum algae liquid per mL. After removing the supernatant, about 0.1 g of microalgae wet biomass is obtained. The water content is determined to be 88%. Add 0.5 mL n-hexane and 1 mL mass fraction to the centrifuge tube It is a 2% methanol solution of sodium hydroxide, transferred to a sealed test tube, heated to 80°C, kept at a constant temperature for 20 minutes, cooled for 5 minutes to room temperature after the reaction, and then added 1 mL of 10% acetyl chloride methanol solution and heated to 80 Keep the temperature at a constant temperature for 20 minutes. After the reaction, cool for 5 minutes to room temperature. Add 0.5 mL of n-hexane and 1 mL of potassium carbonate solution with a mass fraction of 6%, shake and stand. After stratification occurs, all the upper liquid is transferred to the rotary evaporator. Heat to 68℃, distill the n-hexane out, and the remaining li...
Embodiment 2
[0031] Centrifuge at 4500rpm for 10min to collect the cell density to 10 7 100 mL of Isochrysis sphaeroides per mL. After removing the supernatant, about 0.1 g of microalgae wet biomass is obtained. The water content is determined to be 90%. Add 0.5 mL of n-hexane and 1 mL of mass fraction to the centrifuge tube. Transfer 2% methanol solution of sodium hydroxide to a sealed test tube, heat to 80℃, keep constant temperature for 20min, after the reaction, cool for 5min to room temperature, then add 1mL 10% acetyl chloride methanol solution and heat to 80℃ , Constant temperature for 20 minutes, cooling for 5 minutes to room temperature after the completion of the reaction, adding 0.5 mL of n-hexane and 1 mL of potassium carbonate solution with a mass fraction of 6%, shaking and standing, after stratification, all the upper liquid is transferred to the rotary evaporator, heating When the temperature reaches 68°C, the n-hexane is distilled out, and the remaining liquid is fatty acid ...
Embodiment 3
[0033] In this example, glyceryl heptadecanoate is used as the test sample. Most of the fatty acids in microalgae are in the form of triacylglycerides, and glyceryl heptadecanoate is one of them. Therefore, by measuring glyceryl heptadecanoate The conversion rate of ester into methyl heptadecanoate can further prove the accuracy of the present invention. Take 0.5 mL of glyceryl heptadecanoate in toluene solution (containing 0.1 mg of glyceryl heptadecanoate, equivalent to about 0.1 mg of methyl heptadecanoate) in a test tube, and add 0.5 mL of n-hexane and 1 mL of mass 2% sodium hydroxide methanol solution, after sealing, heat to 80℃, keep the temperature for 20min, after the reaction, cool for 5min to room temperature, then add 1mL 10% acetyl chloride methanol solution, heat to 80℃, keep the temperature for 20min After the reaction, cool for 5 minutes to room temperature, add 0.5 mL of methyl nonadecanate n-hexane solution (containing 0.1 mg of methyl nonadecanate) and 1 mL of...
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