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A kind of chromium-rich aspergillus oryzae and its production method and application

A production method and technology of chrome-rich rice are applied in the field of Aspergillus oryzae to achieve the effects of easy filtration, fast growth and promotion of the growth of broilers

Active Publication Date: 2018-03-30
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aspergillus oryzae is often used in the fermentation of condiments such as soy sauce, and there are rare reports on the use of Aspergillus oryzae to prepare organic chromium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Inoculate the spores from the preserved slant of Aspergillus oryzae on the tiger red agar plate, culture at 30-32°C for 48 hours, take 1cm*1cm bacterial block and inoculate it into a 500mL Erlenmeyer flask containing 100mL seed medium, and inoculate at 32°C , Shaking culture at 120r / min for 36 hours to obtain seed solution. The weight percentage of the seed medium consists of: 1% sucrose, 0.6% yeast extract, CrCl 3 ·6H 2 O 0.05%, the balance is water, and the pH is natural.

[0025] Inoculate 100mL of seed solution into a 5L mechanically stirred fermenter with 3L of fermentation medium, at 30-32°C, 200r / min, with an air flow of 3L / min, and cultivate for 48 hours. The weight percentage of the fermentation medium is composed of: sucrose 2 %, yeast extract 1.0%, mannan oligosaccharides 0.01%, CrCl 3 .6H 2 O 0.08%, the balance is water, the pH is natural, collect the culture, filter the filter cake (that is, the bacteria), wash it twice with deionized water, dry it at 6...

Embodiment 2

[0027] Pick spores from the preserved slant of Aspergillus oryzae with an inoculation loop and inoculate them on Tiger Red Agar, and cultivate them at 30-32°C for 56 hours. Take 1 cm * 1 cm bacterial block and inoculate it into a 500 mL Erlenmeyer flask filled with 100 mL of seed medium, and culture at 30-32 °C with shaking at 150 r / min for 48 hours. The composition of the seed medium is: 3% sucrose, 1.2% yeast extract, CrCl 3 .6H 2 O 0.05%, the balance is water, and the pH is natural.

[0028] 100mL seed liquid was inoculated in a 5L mechanically agitated fermenter equipped with 4L fermentation medium (fermentation medium composition: 3% sucrose, 1.2% yeast extract, 0.015% mannan oligosaccharide, CrCl 3 ·6H 2 O 0.1%, the balance is water, pH is natural), 30~32°C, 250r / min, ventilation rate 3.5L / min, cultivated for 56 hours, collected the bacteria, dried and crushed, the finished product is obtained, the chromium content in the finished product was 2.82%.

Embodiment 3

[0030] Use an inoculation loop to pick spores from the preserved slant of Aspergillus oryzae and inoculate them on Tiger Red Agar, and culture them at 30-32°C for 64 hours. Get 1 cm * 1 cm bacterial block and inoculate it into 150 mL seed liquid medium (composed of 2% sucrose, 0.9% yeast extract, CrCl 3 ·6H 2 O 0.05%, the balance is water, pH natural) in a 500mL Erlenmeyer flask, 30 ~ 32 ℃, 130r / min shaking culture for 40 hours.

[0031] 150mL seed solution was inoculated in a 5L mechanically stirred fermenter with 3.5L fermentation medium (fermentation medium composition: 4% sucrose, 1.5% yeast extract, 0.02% mannan oligosaccharide, CrCl 3 ·6H 2 O 0.12%, the balance is water, pH is natural), 30~32°C, 350r / min, ventilation rate 4.0L / min, cultivated for 50 hours, collected the bacteria, dried and crushed, the finished product is obtained, the chromium content in the finished product is 2.95%.

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Abstract

The invention discloses a chromium-rich aspergillus oryzae and its production method and application. The chromium-rich aspergillus oryzae is prepared by the following steps: taking spores from a preserved slant of aspergillus oryzae and inoculating them on tiger red agar for activation; taking 1 cm * 1 cm The activated bacterial block is inoculated into a triangular flask containing a seed medium to prepare a seed solution; the seed solution of 1 to 4% of the fermentation medium volume is inoculated in a mechanically stirred fermenter equipped with a fermentation medium for fermentation; The material was filtered, and the filter cake was dried and crushed to obtain chromium-rich Aspergillus oryzae. The bacterium prepared by the invention has a high chromium content of 26-30g per kilogram of the thallus; the production method is simple and efficient, and the strains do not need to be domesticated or fed with inorganic chromium ions, which simplifies the operation.

Description

technical field [0001] The invention relates to a kind of aspergillus oryzae, specifically a chromium-rich aspergillus oryzae, and also relates to a production method for producing chromium-rich thallus by culturing aspergillus oryzae and the application of the chromium-rich thallus. Background technique [0002] Chromium is an essential trace element in animals and an important part of glucose tolerance factor. Chromium has the function of regulating fat, sugar and protein metabolism, and can improve the meat quality of poultry and livestock. Inorganic chromium is highly toxic to animals and difficult to absorb; organic chromium is absorbed quickly and is safe. As a new type of feed additive, organic chromium has received extensive attention and application. Commonly used forms of organic chromium include amino acid chelated chromium, cell chromium, etc. Among them, the chromium-enriched cells produced by microbial culture have become a research hotspot for organic chromiu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23K10/18A23K50/75C12R1/69
Inventor 杨柳吴俊贤刘国庆邓杰勇
Owner HEFEI UNIV OF TECH
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