Method for preparing tobacco pineapple flavor by processing pineapple juice by using mucor
A technology of pineapple juice and mucormycetes, which is applied in the treatment of tobacco, essential oils/spices, tobacco, etc., can solve the problems of cigarette irritation, slow filtration speed, residual negative effects, etc., and achieve the reduction of pectin content, clarification of filtrate, and filtration convenient effect
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Embodiment 1
[0021] 1. Preparation of pineapple juice: Take 500g of fresh pineapple, add 5 times the volume of water and wash it twice. Using a juice extractor to beat the pineapple, collect the slurry, and sterilize it for 15 minutes to obtain pineapple juice;
[0022] 2. Preparation of mucormycetes preparation: according to the mass ratio of mucormyces preparation (purchased from Yada Group Science, Industry and Trade Co., Ltd.) and sterile water to 1:100, a mucormyces biological preparation solution was prepared;
[0023] 3. Inoculation: under aseptic conditions, inoculate the mucormycetes preparation in the sterilized pineapple juice, and the inoculation weight ratio is 0.5%;
[0024] 4. Fermentation: ferment the inoculated pineapple juice for 12 days at a pH value of 5, a stirring speed of 50 rpm, an aeration rate of 0.2 VVM, and a fermentation temperature of 30°C;
[0025] 5. Separation and purification: Centrifuge the fermented liquid obtained after fermentation at a centrifugal ra...
Embodiment 2
[0031] 1. Preparation of pineapple juice: Take 500g of fresh pineapple, add 5 times the volume of water and wash it twice. Using a juicer to beat the pineapple, collecting the slurry, and sterilizing it for 20 minutes to obtain pineapple juice;
[0032] 2. Preparation of mucormycetes preparation: according to the mass ratio of mucormyces preparation (purchased from Yada Group Science, Industry and Trade Co., Ltd.) and sterile water to 1:100, a mucormyces biological preparation solution was prepared;
[0033] 3. Inoculation: under aseptic conditions, the mucormycosis preparation is inoculated in the sterilized pineapple juice, and the inoculation weight ratio is 1%;
[0034] 4. Fermentation: ferment the inoculated pineapple juice for 8 days at a pH value of 7, a stirring speed of 80 rpm, an aeration rate of 0.5 VVM, and a fermentation temperature of 35°C;
[0035] 5. Separation and purification: centrifuge the fermented liquid obtained after fermentation at a centrifugal rate ...
Embodiment 3
[0041] 1. Preparation of pineapple juice: Take 500g of fresh pineapple, add 5 times the volume of water and wash it twice. Using a juicer to beat the pineapple, collecting the slurry, and sterilizing it for 30 minutes to obtain pineapple juice;
[0042] 2. Preparation of mucormycetes preparation: according to the mass ratio of mucormyces preparation (purchased from Yada Group Science, Industry and Trade Co., Ltd.) and sterile water to 1:100, a mucormyces biological preparation solution was prepared;
[0043] 3. Inoculation: under aseptic conditions, the mucormycosis preparation is inoculated in the sterilized pineapple juice, and the inoculation weight ratio is 5%;
[0044] 4. Fermentation: ferment the inoculated pineapple juice for 5 days at a pH value of 8, a stirring speed of 100 rpm, an aeration volume of 1 VVM, and a fermentation temperature of 42°C;
[0045] 5. Separation and purification: centrifuge the fermented liquid obtained after fermentation at a centrifugal rate...
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