Silk protein scaffold material and preparation method thereof

A scaffold material, silk protein technology, applied in medical science, surgery, etc., can solve the problems of high hardness, uncontrollable surface structure of porous scaffold pore wall, unsuitable for endothelial cell proliferation and blood vessel formation, etc.

Active Publication Date: 2015-10-14
丝纳特(苏州)生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, silk protein porous scaffolds prepared by salting-out method and induced by organic solvents such as methanol have the problems of high ha

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  • Silk protein scaffold material and preparation method thereof
  • Silk protein scaffold material and preparation method thereof
  • Silk protein scaffold material and preparation method thereof

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[0029] The invention provides a method for preparing a silk protein scaffold material, comprising:

[0030] A) concentrating the silk protein solution to obtain a concentrated solution, wherein the mass concentration of silk protein in the concentrated solution is 15% to 35%;

[0031] B) diluting and heat-treating the concentrated solution obtained in step A) to obtain a hydrogel, and the mass concentration of silk protein in the hydrogel is 0.2% to 0.6%;

[0032] C) Mix the concentrated solution obtained in step A) with the hydrogel obtained in step B) to obtain a mixed solution; adjust the pH value of the mixed solution, stir, freeze, and dry to obtain a scaffold material; the pH value is 3 to 4 .

[0033] The silk protein solution of the present invention is a silk protein solution prepared by a conventional method, preferably, the preparation method is specifically:

[0034] a) heating and degumming silk in sodium carbonate solution, washing with water, and drying to obt...

Embodiment 1

[0063] 50g silk in 0.5% Na 2 CO 3 Boil in the solution at 100°C for 60 minutes to remove sericin on the outside of the silk, rinse with deionized water, repeat the above operation 3 times, and then dry the silk at 60°C. Weigh 15 g of degummed silk after the above treatment and dissolve in 100 mL of LiBr solution with a mass concentration of 9.3 mol / L, and dissolve at 60 °C for 4 hours. Then use a dialysis bag with a molecular weight cut-off of 3500 to immerse in deionized water and dialyze for 3 days, during which the water is changed every two hours to remove LiBr in the solution, thereby obtaining a pure silk protein solution with a mass concentration of 6%.

[0064] Concentrate the silk protein solution at 60° C. for 48 hours, put it in a fume hood and concentrate for 48 hours, and the mass concentration of the silk protein solution after concentration is 28%. Then the mass fraction of the silk protein aqueous solution was diluted to 0.3%, and then treated at 60° C. for 2...

Embodiment 2

[0087] 50g silk in 0.5% Na 2 CO 3 Boil in the solution at 100°C for 60 minutes to remove sericin on the outside of the silk, rinse with deionized water, repeat the above operation 3 times, and then dry the silk at 60°C. 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 mL of a LiBr solution with a mass concentration of 9.3 mol / L, and dissolved at 60° C. for 4 hours. Then use a dialysis bag with a molecular weight cut-off of 3500 to immerse in deionized water and dialyze for 3 days, during which the water is changed every two hours to remove LiBr in the solution, thereby obtaining a pure silk protein solution with a mass concentration of 6%.

[0088] Concentrate the silk protein solution at 60° C. for 48 hours, put it in a fume hood and concentrate for 48 hours, and the mass concentration of the silk protein solution after concentration is 28%. Then the mass fraction of the silk protein aqueous solution was adjusted to 0.5%, and then treated...

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Abstract

The present invention provides a silk protein scaffold material preparation method, which comprises: A) concentrating a silk protein solution to obtain a concentrated solution, wherein the mass concentration of the silk protein in the concentrated solution is 15-35%; B) carrying out diluting and heat treatment on the concentrated solution obtained in the step A) to obtain a hydrogel, wherein the mass concentration of the silk protein in the hydrogel is 0.2-0.6%; and 3) mixing the concentrated solution obtained in the step A) and the hydrogel obtained in the step B) to obtain a mixed solution, adjusting the pH value of the mixed solution, stirring, freezing, and drying to obtain the scaffold material, wherein the pH value is 3-6. According to the present invention, the concentrated solution and the hydrogel are mixed and the self-assembly behavior of the silk protein is used to change the silk protein secondary structure, such that the porosity is high; the silk protein solution is selected, such that the biodegradability is good; the pH value is adjusted so as to destroy the hydrophobic interaction force; and the silk protein scaffold material has characteristics of low mechanical property and low crystallinity, and is suitable for angiogenesis.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a silk protein scaffold material and a preparation method thereof. Background technique [0002] Defects and lesions of tissues and organs caused by various traumas and diseases are a major problem affecting human health. At present, organ transplantation is often used clinically to treat tissue and organ defects. Insufficient donors in tissue and organ transplantation, defect repair and functional reconstruction of tissues and organs have become urgent problems to be solved in the field of life sciences. The basic principle of tissue engineering is to use three-dimensional scaffold materials to simulate the physiological environment in vivo, culture cells in vitro, and then implant cell-scaffold composites into the body to replace diseased tissues and organs, so as to achieve the purpose of wound repair and functional reconstruction. [0003] The construction of fun...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/14
Inventor 吕强宁洪艳
Owner 丝纳特(苏州)生物科技有限公司
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