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Composite nanometer photoacoustic contrast agent based on polybutyl acrylate, and preparation process thereof

A polybutyl acrylate and nano-light technology, which can be used in preparations and pharmaceutical formulations for in vivo experiments, can solve problems such as poor stability and short metabolic cycle.

Inactive Publication Date: 2015-10-21
WENZHOU INST OF BIOMATERIALS & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the poor stability of these two dyes in vivo and the short metabolic cycle, it is necessary to construct carriers carrying dye molecules to realize their clinical application.

Method used

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  • Composite nanometer photoacoustic contrast agent based on polybutyl acrylate, and preparation process thereof
  • Composite nanometer photoacoustic contrast agent based on polybutyl acrylate, and preparation process thereof
  • Composite nanometer photoacoustic contrast agent based on polybutyl acrylate, and preparation process thereof

Examples

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Embodiment 1

[0026] Example 1: Butyl acrylate (1.5 ml), dextran solution (100 ml), and methylene blue (6.4 mg) were fed into a 250 ml beaker in proportion, and the pH of the reaction system was adjusted to 2.0-3.0 with 1M hydrochloric acid solution. After stirring at room temperature, dilute sodium hydroxide solution was added to terminate the reaction. The reaction system was left to stand for 4-7 hours, then the entire reaction solution was transferred to a 50 ml centrifuge tube for centrifugation and purification, the supernatant was discarded, washed with high-purity water, and vortexed several times. Repeat the centrifugation step at least 2 times to obtain a pure product of the composite nano-photoacoustic contrast agent after concentration, which can be vacuum freeze-dried or re-dispersed in high-purity water and stored at room temperature. The concentration of methylene blue in the composite nano-photoacoustic contrast agent is 7.6 μg / ml. Based on the principle of dynamic light s...

Embodiment 2

[0027] Example 2: Butyl acrylate (1.5 ml), dextran solution (100 ml), and indocyanine green (4.9 mg) were fed into a 250 ml beaker in proportion, and the pH of the reaction system was adjusted to 2.0-3.0 with 1M hydrochloric acid solution . After stirring at room temperature, dilute sodium hydroxide solution was added to terminate the reaction. The reaction system was left to stand for 4-7 hours, then the entire reaction solution was transferred to a 50 ml centrifuge tube for centrifugation and purification, the supernatant was discarded, washed with high-purity water, and vortexed several times. Repeat the centrifugation step at least 2 times to obtain a pure product of the composite nano-photoacoustic contrast agent after concentration. The concentration of indocyanine green in the composite nano-photoacoustic contrast agent is 5.8-6 micrograms / ml, preferably 5.8 micrograms / ml. Vacuum freeze-dry or re-disperse in high-purity water and store at room temperature. The particl...

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Abstract

The present invention discloses a composite nanometer photoacoustic contrast agent based on polybutyl acrylate, and a preparation process thereof, wherein the main component is a biomedical material polybutyl acrylate, the entrapped component is methylene blue or indocyanine green, the particle diameters respectively are 525.1 nm and 272.0 nm, and the potentials respectively are -4.52 mV and -5.98 mV. According to the present invention, the preparation process has characteristics of mild process, environmental protection, low energy consumption, no generation of three-waste, radiation, noise and other pollutions, simple separation and purification process operation, high yield, high entrapping rate and easy industrial production achieving, and is the universal process for preparing the composite nanometer photoacoustic contrast agent; and the composite nanometer photoacoustic contrast agent has characteristics of good biocompatibility, good safety and high photoacoustic imaging sensitivity, such that the composite nanometer photoacoustic contrast agent is expected to be widely used in the fields of biomedical imaging, targeted diagnosis and treatment, drug screening and optimization, in vivo labeling and tracing and the like, and has potential values in the fields of personalized medicine and the like.

Description

technical field [0001] The invention relates to a composite nano photoacoustic contrast agent based on polybutylacrylate and a preparation process thereof. Background technique [0002] Photoacoustic imaging (Photoacoustic Imaging, PAI) is a new biomedical imaging technology in the past ten years. An imaging method for images, which combines the advantages of high sensitivity of optical imaging and deep penetration of acoustic imaging, and can perform high-resolution and high-contrast imaging of deep tissues, so it has become one of the most promising imaging modes. Since a variety of endogenous biochemical substances in organisms, such as deoxyhemoglobin, oxygenated hemoglobin, melanin, oil, water, etc., can be excited by excitation light of specific wavelengths, and most of these components are closely related to physiological functions, photoacoustic imaging It can sensitively reflect the physiological structure of organisms and provide rich biological information such a...

Claims

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Application Information

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IPC IPC(8): A61K49/00
Inventor 刘哲李宜鸿宋远会石长灿汪婕
Owner WENZHOU INST OF BIOMATERIALS & ENG
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