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Poly(L-lactide-co-epsilon-caprolactone)nano-fiber nerve conduit and preparation method thereof

A nanofiber and nerve conduit technology, applied in the field of poly(L-lactide-co-ε-caprolactone) nanofiber nerve conduit and preparation, can solve problems such as difficulty in taking the tube and achieve good biocompatibility , complete structure, high matching effect

Active Publication Date: 2015-10-28
山东隽秀生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the limitations of existing nerve guide materials and the difficulty in taking tubes caused by the use of metal rods to directly accept nanofibers, the present invention provides a poly(L-lactide-co-ε-caprolactone) nano The fiber nerve guide and its preparation method, the prepared nerve guide has good biocompatibility and mechanical strength, and the degradation time is appropriate, and a layer of gelatin film is formed on the metal rod, and the gelatin film is removed by water dissolution after the catheter is formed, which can The nerve guide is easily removed, maintaining the integrity of the nerve guide structure

Method used

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  • Poly(L-lactide-co-epsilon-caprolactone)nano-fiber nerve conduit and preparation method thereof
  • Poly(L-lactide-co-epsilon-caprolactone)nano-fiber nerve conduit and preparation method thereof
  • Poly(L-lactide-co-epsilon-caprolactone)nano-fiber nerve conduit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Weigh 0.5g poly(L-lactide-co-ε-caprolactone) (molecular weight 100,000, molar ratio of L-lactide and ε-caprolactone is 80 / 20), dissolve in In 4ml of tetrahydrofuran and N,N-dimethylformamide mixed solvent, the volume ratio of tetrahydrofuran and N,N-dimethylformamide is 70 / 30, and a polymer solution with a concentration of 0.125g / ml is prepared;

[0023] (2) Immerse a metal rod with a diameter of 2mm in a gelatin solution with a concentration of 0.02g / ml, take it out and dry it, and form a gelatin film on the outer layer of the metal rod;

[0024] (3) Set the spinning parameters and perform electrospinning, the spinning voltage is 10KV, the spinning speed is 2ml / h, and the receiving distance is 10cm;

[0025] (4) Take off the metal rod after spinning for 1 hour, put it in 30°C water, dissolve the gelatin film covering the metal rod, and take off the nerve guide. The inner diameter of the prepared nerve guide is 2 mm, and the length is 5 cm. The wall thickness is 0....

Embodiment 2

[0027] (1) Weigh 1g poly(L-lactide-co-ε-caprolactone) (molecular weight 300,000, the molar ratio of L-lactide and ε-caprolactone is 70 / 30), dissolve it in 10ml In the mixed solvent of tetrahydrofuran and N,N-dimethylformamide, the volume ratio of tetrahydrofuran and N,N-dimethylformamide is 50 / 50, and a polymer solution with a concentration of 0.1g / ml is prepared;

[0028] (2) Immerse a metal rod with a diameter of 5mm in a gelatin solution with a concentration of 0.05g / ml, take it out and dry it, and form a gelatin film on the outer layer of the metal rod;

[0029] (3) Set the spinning parameters and perform electrospinning, the spinning voltage is 15KV, the spinning speed is 0.8ml / h, and the receiving distance is 15cm;

[0030] (4) Take off the metal rod after spinning for 5 hours, put it in 40°C water, dissolve the gelatin film covering the metal rod, and take off the nerve guide. The inner diameter of the prepared nerve guide is 5 mm, and the length is 10 cm. The wall thi...

Embodiment 3

[0032] (1) Weigh 2g poly(L-lactide-co-ε-caprolactone) (molecular weight of 500,000, the molar ratio of L-lactide and ε-caprolactone is 50 / 50), dissolve in 15ml In the mixed solvent of tetrahydrofuran and N,N-dimethylformamide, the volume ratio of tetrahydrofuran and N,N-dimethylformamide is 60 / 40, and a polymer solution with a concentration of 0.13g / ml is prepared;

[0033] (2) Immerse a metal rod with a diameter of 10mm in a gelatin solution with a concentration of 0.1g / ml, take it out and dry it, and form a gelatin film on the outer layer of the metal rod;

[0034] (3) Set the spinning parameters and perform electrospinning, the spinning voltage is 20KV, the spinning speed is 2ml / h, and the receiving distance is 20cm;

[0035] (4) After spinning for 6 hours, remove the metal rod, place it in 50°C water, dissolve the gelatin film covering the metal rod, and remove the nerve guide. The inner diameter of the prepared nerve guide is 10 mm, and the length is 8 cm. The wall thick...

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Abstract

The present invention discloses a poly(L-lactide-co-epsilon-caprolactone)nano-fiber nerve conduit and a preparation method thereof. The preparation method comprises: (1) dissolving poly(L-lactide-co-epsilon-caprolactone) in a mixed solvent of tetrahydrofuran and N,N-dimethyl formamide; (2) immersing a nano-fiber acceptable metal rod in a gelatin solution, taking out, and carrying out air-drying so as to form a gelatin film layer on the outer layer of the metal rod; (3) carrying out electrostatic spinning of the poly(L-lactide-co-epsilon-caprolactone) / tetrahydrofuran / N,N-dimethyl formamide solution so as to make the metal rod coated with the gelatin film accept the nano-fibers; and (4) after completing the electrostatic spinning, and immersing the metal rod into warm water to dissolve the gelatin so as to take out the nerve conduit. According to the present invention, the nerve conduit has characteristics of good biocompatibility and good mechanical strength, the conduit wall has rich micropores so as to easily perform nutrient exchange, and the nerve conduit degradation time and the autologous nerve regeneration time are matched; and the preparation method has characteristics of simpleness, no environment pollution, and high economic benefit.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a poly(L-lactide-co-ε-caprolactone) nanofiber nerve guide and a preparation method. Background technique [0002] Peripheral nerve damage can cause a decline or loss of sensory or motor function in patients, and severe cases can lead to paralysis. According to statistics, there are more than 1 million cases of peripheral nerve defects caused by traffic accidents, industrial injuries, sports injuries, and surgical operations in my country every year. At present, there are three main clinical methods for repairing nerve defects: end-to-end suture, autologous nerve transplantation, and artificial nerve conduit bridging. Because the end-to-end suture method has a significant impact on the microcirculation in the nerve, the quality and number of newborn nerves are poor, and the repair effect is not ideal. Although autologous nerve transplantation is the "gold standard...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/50A61L27/56
Inventor 高秀岩姜红任孝敏王爱军敖强
Owner 山东隽秀生物科技股份有限公司
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