Fluorescent microsphere with micelle particle interlayer and surface carboxylation and preparation method thereof
A technology of fluorescent microspheres and carboxylation, which is applied in the field of fluorescent sensing materials to achieve the effects of good dispersion performance, convenient operation, and simplified preparation process
Active Publication Date: 2017-06-13
SUZHOU UNIV
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Problems solved by technology
[0003] The present invention aims at the problems of polyelectrolyte chain interpenetration and self-quenching of fluorescence existing in fluorescent microspheres with core-shell structure assembled by electrostatic interaction, and provides a method that can effectively overcome the deficiencies in the prior art, and has a simple preparation process and a high-quality product. Fluorescent microspheres with stable structure, good shape, micellar particle interlayer and surface carboxylation and preparation method thereof
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Embodiment 1
[0022] This embodiment provides a fluorescent microsphere with a micelle particle interlayer and carboxylated surface, the preparation steps of which are as follows:
[0023] Step 1: Preparation of poly(p-phenylenevinylene) precursor salt solution
[0024] Referring to the literature Macromol. Rapid Commun. 2013, 34, 102-108, the synthesis of poly-p-phenylene vinylene precursor: the p-phenylene vinylene monomer was catalyzed in an oxygen-free environment and sodium hydroxide aqueous solution, and ice-water bath After reacting in the medium for one hour, dialyze with deionized water 5 to 6 times to obtain an aqueous solution of the poly(p-phenylenevinylene) precursor. Use a pipette gun to measure 2.5mL of poly(p-phenylenevinylene)precursor aqueous solution, add 0.02925g of sodium chloride to it, shake to dissolve, and obtain a colorless and transparent poly(p-phenylenevinylene) precursor salt solution.
[0025] Step 2: Preparation of poly(acrylic acid-styrene) micellar particl...
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Abstract
The invention discloses a fluorescent microsphere with a micelle particle interlayer and carboxylated surface and a preparation method thereof, belonging to the technical field of fluorescent sensing materials. Poly(acrylic acid-styrene) block copolymers are self-assembled in a mixed solvent of tetrahydrofuran and water to obtain a solution of poly(acrylic acid-styrene) polymer micelles; Vinylbenzene cross-linked microspheres are the core, driven by electrostatic force on the surface of the core, layer by layer self-assembly and alternate deposition of p-phenylene vinylene precursor and poly(acrylic acid-styrene) micellar particles, and then heat-treated to obtain p-Phenylenevinylene / poly(acrylic-styrene) micellar shell. The fluorescent microsphere provided by the invention has a maximum emission wavelength of 510-520 nm; the fluorescent microsphere has a stable structure, good surface appearance and dispersion performance; the preparation process is simple, the operation is convenient, and it is suitable for industrial production, and the product can be applied to biological detection , medical diagnosis and other fields.
Description
technical field [0001] The invention relates to a polymer fluorescent microsphere and a preparation method thereof, in particular to a fluorescent microsphere with a micelle particle interlayer and carboxylated surface and a preparation method thereof, belonging to the technical field of fluorescent sensing materials. Background technique [0002] As the core sensitive element of liquid suspension chip technology, functionalized fluorescent-encoded microspheres undertake the tasks of molecular recognition and signal expression. Therefore, the research and development of chemical and biological modification methods on the surface of microsphere carriers and the preparation of functionalized fluorescent microspheres with excellent performance are important contents for the construction of high-sensitivity and high-selectivity liquid phase suspension chip technology. The method of using electrostatic interaction to adsorb conjugated polyelectrolytes to the surface of microspher...
Claims
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IPC IPC(8): C09K11/06C09K11/02
Inventor 范丽娟邱钿邵亚
Owner SUZHOU UNIV