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Visual chip and preparation method thereof and method for chip visualization

A technology of chips and biochips, applied in the field of biochips and chip visualization, can solve problems such as complexity, inconvenient interpretation of results, error-prone, etc., and achieve the effect of simple operation, convenient observation and reading, and less error-prone

Inactive Publication Date: 2015-10-28
JIANGSU HUNTARRAY BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the most common method on the market is to use a chip spotter to place the probe on the corresponding chip, and then judge the detection result by hybridization and color development of the substrate during the detection process; when the type detected at the same time When there are too many types, such as hpv genotyping detection, more than 30 types may be involved at the same time. After hybridization and color development on the chip, each type needs to be determined according to the pre-set layout, which is more complicated and It is easy to make mistakes and bring inconvenience to the interpretation results

Method used

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  • Visual chip and preparation method thereof and method for chip visualization
  • Visual chip and preparation method thereof and method for chip visualization
  • Visual chip and preparation method thereof and method for chip visualization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The preparation of embodiment 1 chip

[0043] 1. Procurement of probes and equipment: All probes were purchased from Shanghai Sangon Biotechnology Co., Ltd., and the spotting instrument was Jingxin PersonalArrayer 16 personal spotting instrument, which was purchased from Boao Biological Co., Ltd.

[0044] 2. Setting of the probe style on the chip: according to the project requirements, set the corresponding program on the spotting instrument software, that is, set the spotting style of the probe to be spotting as figure 1 The numbers and letters shown in .

[0045] 3. Spotting: place the film to be spotted on the film placement place of the spotting instrument, and spot the sample according to the settings, such as figure 1 , and carry out drying treatment. That is, the corresponding chip is obtained.

[0046] Spotting of the probe: use the setting program of the spotting instrument to spot the probe solution on the chip with the name of the corresponding detection i...

Embodiment 2

[0047] The detection of embodiment 2 human papillomavirus (HPV) nucleic acid typing (28 types)

[0048] 1. Purchase of HPV nucleic acid typing primers and probes: including two sets of primers and 30 sets of specific probes, of which two sets of primers are used The other set of probes is hybridization reaction quality control probes, as long as the hybridization process is normal, it can be displayed in the end; the remaining 28 sets of probes are used for typing of 28 specific types , were purchased from Shanghai Sangon Biotech.

[0049] 2. Extraction of HPV nucleic acid samples, the HPV nucleic acid samples are extracted from cervical exfoliated cell specimens of HPV patients in a hospital in Jiangsu Province.

[0050] 3. PCR amplification: Prepare a PCR system according to the reagents and nucleic acid samples in steps 1 and 2, a total of 20ul system, including 19ul of PCR reaction solution and 1ul of template. Carry out PCR amplification according to the following proce...

Embodiment 3

[0059] Preparation and detection of embodiment 3 protein chip

[0060] 1. Preparation of the protein chip: immobilize the capture antibody on the solid phase support in the form of the pattern to be displayed (including numbers and letters). The solid phase support can be a glass slide or a nitrocellulose membrane.

[0061] 2. Sample treatment: label the sample with protein biotin, and covalently couple the activated biotin to the amino group of the protein.

[0062] 3. After the biotin-labeled sample is added to the chip and incubated with the reaction, the capture antibody can bind to the specific protein.

[0063] 4. Add HRP-streptavidin to combine with chemiluminescent substrate or fluorescent dye-streptavidin as signal detection, and observe the corresponding experimental results on the chip after 10 minutes of reaction. Color rendering effect such as image 3 As shown, the test results can be read directly without providing another comparison table.

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Abstract

The invention relates to a visual biochip. The visual biochip is characterized in that specific biological probes are arrayed on a supported type chip substrate in a pattern mode. The invention further relates to a preparation method of the biochip and a method for chip visualization.

Description

technical field [0001] The invention relates to a chip, in particular to a biological chip. The invention also relates to a preparation method of the biological chip and a method for visualizing the chip. Background technique [0002] Chip technology is an emerging industry, mainly divided into gene chip technology, flip chip technology, biochip technology, tissue chip technology, protein chip technology, protein chip technology, DNA chip technology, liquid phase chip technology, chip packaging technology, etc. . [0003] Gene chip technology immobilizes a large number of probe molecules on a solid support at the same time, and uses the characteristics of hybridization and pairing of nucleic acid molecules to efficiently interpret and analyze the sequence information of DNA samples. Gene chips are also called DNA chips or DNA microarrays. The principle is to immobilize a large number of probe molecules of a specific sequence densely and orderly on the correspondingly trea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B40/08C40B40/10C40B40/12C40B40/00C40B50/00C12Q1/68G01N33/68
CPCC12Q1/68G01N33/68
Inventor 杨华卫刘泽涛古恒森
Owner JIANGSU HUNTARRAY BIOTECH CO LTD
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