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A kind of propagation method of golden clematis seedling

A technology for clematis and seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high production cost, low quality of seedlings, large manpower and material resources, etc. The effect of shortage of seedling resources, simple steps, and shortened production cycle

Active Publication Date: 2017-04-26
江苏灵福嘉叶生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing method of applying plant tissue culture to produce clematis seedlings consumes a lot of manpower and material resources, the production cost remains high, and the cultivation period is long, and the quality of seedlings is not high, which still cannot meet the needs of drug farmers.
In addition, in the Chinese patent document CN101213942A, a method for rapid propagation of one-step seedling growth by tissue culture of medicinal Auranthus clematis is disclosed, but the cultivation time is too long and the efficiency is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The multiplication method of golden clematis seedling, carries out according to the following steps:

[0034] (1) Cultivation and inoculation of explants:

[0035] (1.1) Explant collection and cleaning: select a height of 7 to 10 cm, with 4 to 6 leaves, and 3 to 4 internodes of the original plant of Aurantia clematis, on a sunny day after three consecutive sun exposures, Collect the whole plant as an explant, first put the explant in a 1000ml beaker, put a small amount of washing powder to clean it, and then rinse it repeatedly with clean water until there is no foam;

[0036] (1.2) Sterilization of explants: Prepare 50% (w / v) ethanol solution, 0.1% (w / v) mercuric chloride solution and sterile water. Soak the body bubble in 50% (w / v) ethanol solution for 5 seconds, then quickly remove it, soak it in 0.1% (w / v) mercuric chloride solution for 12 minutes, and finally fill it with sterile water in a sterile container. After repeated washing 3 times, control the water and ...

Embodiment 2

[0053] The multiplication method of golden clematis seedling, carries out according to the following steps:

[0054] (1) Cultivation and inoculation of explants:

[0055] (1.1) Explant collection and cleaning: select a height of 7 to 10 cm, with 4 to 6 leaves, and 3 to 4 internodes of the original plant of Aurantia clematis, on a sunny day after three consecutive sun exposures, Collect the whole plant as an explant, first put the explant in a 1000ml beaker, put a small amount of washing powder to clean it, and then rinse it repeatedly with clean water until there is no foam;

[0056] (1.2) Sterilization of explants: Prepare 50% (w / v) ethanol solution, 0.1% (w / v) mercuric chloride solution and sterile water. Soak the body bubble in 50% (w / v) ethanol solution for 5 seconds, then quickly remove it, soak it in 0.1% (w / v) mercuric chloride solution for 12 minutes, and finally fill it with sterile water in a sterile container. After repeated washing 3 times, control the water and ...

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PUM

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Abstract

The invention relates to an anoectochilus roxburghii seedling propagation expanding method. The method comprises the steps of explant culturing and grafting, high-efficiency propagation expanding through a shaking table, growing culture in a solid culture medium, rooting culture in the solid culture medium, seedling hardening and planting. MS macroelements and MS microelements are adopted for acting on mineral nutrition of culture; sugar serving as a carbon source of plant cells provides a source for building up cellulose, and forms a certain degree of osmotic pressure on the culture medium, so that the culture starts growing; 6-BA serving as cytokinin promotes new bud fission of plants; NAA serving as an induction factor is used cooperatively with the 6-BA; KT serving as kinetin prevents the plants from being vitrified. The steps of the propagation expanding method are simple, the production cycle is greatly shortened compared with that of a traditional technology, cost is low, the process is environmentally friendly and free from pollution, the method is suitable for industrialization production, the seedling cultivation efficiency of anoectochilus roxburghii can be improved greatly, and the quality of anoectochilus roxburghii products in the whole market can be improved.

Description

technical field [0001] The invention relates to a method for multiplying clematis seedlings, and belongs to the technical field of efficiently expanding and multiplying clematis seedlings. Background technique [0002] Anoecthilus roxburghii (Anoecthilus roxburghii) is a perennial herbaceous plant of the genus Orchidaceae. It is also known as Anoecthilus, Anoecthilus, Anoecthilus, and Anoecthilus. It usually grows in the fertile leaf-rotted soil under the shady broad-leaved forest. It is a traditional precious medicinal material in my country. It has the effects of cooling and detoxifying, nourishing yin and reducing fire, reducing inflammation and relieving pain, and has no toxic and side effects. It is known as the "King of Medicine" among the people. reputation. The plant height of clematis is 8-20cm, the plant type is small and exquisite, the leaf shape is beautiful, the leaf veins are golden yellow, arranged in a net shape, and it is an indoor foliage treasure with high...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 高蕴珺
Owner 江苏灵福嘉叶生物科技有限公司