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Fluorescence coded microspheres based on long-life composite quantum dots and preparation method thereof

A fluorescent coding and composite quantum technology, which is applied in the preparation of microspheres, microcapsule preparations, chemical instruments and methods, etc., can solve the problems of difficult coding and unstable fluorescence intensity, achieve good biocompatibility and improve biological safety. , the effect of improving stability

Active Publication Date: 2015-11-04
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the emission wavelengths of this kind of quantum dot fluorescently encoded microspheres are mostly in the visible light region, and visible light can only penetrate tissues with a thickness of millimeters at most. The fluorescence intensity is unstable and it is difficult to encode correctly. These two defects greatly limit the application of quantum dot fluorescent encoding microspheres in the biological field

Method used

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  • Fluorescence coded microspheres based on long-life composite quantum dots and preparation method thereof
  • Fluorescence coded microspheres based on long-life composite quantum dots and preparation method thereof
  • Fluorescence coded microspheres based on long-life composite quantum dots and preparation method thereof

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Embodiment 1

[0073] The preparation method of the first quantum dot specifically comprises the following steps:

[0074] (1) Preparation of Cd 2+ CdCl with a concentration of 0.1mol / L 2 Aqueous solution, a concentration of 0.2mol / L 3-mercaptopropionic acid aqueous solution, Cu 2+ CuCl with a concentration of 0.1mol / L 2 aqueous solution, spare;

[0075] (2) Newly prepared NaHTe solution: NaBH with a molar ratio of 4.5:1 4 Dissolve Te powder in ultrapure water and react at room temperature for 5 hours to obtain NaHTe solution;

[0076] (3) Preparation of CdTe nucleus: take 5mL CdCl 2 Solution, 4.5mL of 3-mercaptopropionic acid solution was fixed to 30mL, transferred to a 50mL three-necked flask, adjusted to pH 10.5 with NaOH solution, passed argon gas into the three-necked flask for deoxygenation for 1 h, added 100 μL of newly prepared NaHTe solution, and Store at 4°C for 17 hours. Add 60mL of ethanol, centrifuge at 10000r / min for 15min, repeat centrifugation and alcohol washing three...

Embodiment 2

[0084] The preparation method of the first quantum dot specifically comprises the following steps:

[0085] (1) Preparation of Cd 2+ CdCl with a concentration of 0.1mol / L 2 Aqueous solution, a concentration of 0.2mol / L 3-mercaptopropionic acid aqueous solution, Cu 2+ Water CuCl with a concentration of 0.1mol / L 2 solution, spare;

[0086] (2) Newly prepared NaHTe solution: NaBH with a molar ratio of 4.5:1 4 Dissolve Te powder in ultrapure water and react at room temperature for 5 hours to obtain NaHTe solution;

[0087] (3) Preparation of CdTe core: Take 5mL CdCl 2 Solution, 4.5mL of 3-mercaptopropionic acid solution was fixed to 30mL, transferred to a 50mL three-necked flask, adjusted to pH 10.5 with NaOH solution, passed argon gas into the three-necked flask for deoxygenation for 1 h, added 100 μL of newly prepared NaHTe solution, and Store at 4°C for 17 hours. Add 60mL of ethanol, centrifuge at 10000r / min for 15min, repeat centrifugation and alcohol washing three time...

Embodiment 3

[0094] The preparation method of the first quantum dot specifically comprises the following steps:

[0095] (1) Preparation of Cd 2+ CdCl with a concentration of 0.1mol / L 2 Aqueous solution, a concentration of 0.2mol / L 3-mercaptopropionic acid aqueous solution, Cu 2+ CuCl with a concentration of 0.1mol / L 2 aqueous solution, spare;

[0096] (2) Newly prepared NaHTe solution: NaBH with a molar ratio of 4.5:1 4 Dissolve Te powder in ultrapure water and react at room temperature for 5 hours to obtain NaHTe solution;

[0097] (3) Preparation of CdTe core: Take 5mL CdCl 2 Solution, 4.5mL of 3-mercaptopropionic acid solution was fixed to 30mL, transferred to a 50mL three-necked flask, adjusted to pH 10.5 with NaOH solution, passed argon gas into the three-necked flask for deoxygenation for 1 h, added 100 μL of newly prepared NaHTe solution, and Store at 4°C for 17 hours. Add 60mL of ethanol, centrifuge at 10000r / min for 15min, repeat centrifugation and alcohol washing three ti...

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Abstract

The invention provides fluorescence coded microspheres based on long-life composite quantum dots and a preparation method thereof. The fluorescence coded microspheres based on long-life composite quantum dots comprise agarose microspheres, first quantum dots and second quantum dots; the first quantum dots and the second quantum dots are combined with the agarose microspheres by virtue of chemical bonds; each first quantum dot has a core-shell structure which takes cadmium telluride as a core and cadmium sulfide doped with copper ions as a shell; the doping molar fraction of the copper ions in the cadmium sulfide doped with the copper ions is 0.5%-2%, the size of the core is 1.8nm-2.2nm, the thickness of the shell is 2nm-4nm, the emission wavelength range of the first quantum dots is 700nm-910nm, and the fluorescent life is 0.8-1.2 microseconds; and the emission wavelength range of the second quantum dots is 700nm-820nm, and the fluorescent life is 100-220 nanoseconds. The invention further provides a preparation method of the fluorescence coded microspheres based on the long-life composite quantum dots.

Description

technical field [0001] The invention belongs to the technical field of biological analysis, in particular to a fluorescent coded microsphere based on long-life composite quantum dots and a preparation method thereof. Background technique [0002] Semiconductor quantum dots (referred to as QDs, also known as semiconductor nanocrystals) have attracted the attention of researchers in the fields of photoelectric conversion and life science due to their unique optical and electrical properties. Quantum dots are combined into the hollow polymer beads to form quantum dot fluorescence-encoded microspheres with different spectral characteristics and brightness characteristics that can be marked on biological macromolecules. When a single wavelength light source is used to illuminate quantum dot fluorescence-encoded microsphere , can simultaneously excite quantum dots of different sizes and different numbers, so that they emit light of different wavelengths and different fluorescence ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02B01J13/02C09K11/88C09K11/56C09K11/06
Inventor 蔡林涛陈驰张鹏飞高冠慧高笃阳
Owner SHENZHEN INST OF ADVANCED TECH
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