A chromogenic fungal 1,3-β-d-glucan detection kit for human body fluid

Abstract

The invention relates to a developing-process fungus 1,3-beta-D-glucan detection kit for human body fluid. The developing-process fungus 1,3-beta-D-glucan detection kit comprises a reaction main agent, a main agent compound solution, a sample treatment solution, heat-source-free water, a standard product and a quality control product, wherein the reaction main agent takes horseshoe crab blood cells as a main raw material and contains G factors, coagulase, coagulase zymogen and a polypeptide developing substrate; the polypeptide developing substrate is synthesized tripeptide or tetrapeptide with a Gly-Arg tail end connected with a PNA; the polypeptide developing substrate is subjected to enzyme digestion by adopting the coagulase; after the free paranitroaniline (PNA) is generated, a microplate reader is used for directly detecting so that a detection route is shortened and the cost is reduced; the microplate reader is used for carrying out a velocity-method enzyme kinetics detection method so that the sensitivity is relatively high when being compared with a nephelometry detection method; and the reaction main agent is not easily interfered by protein in a body fluid sample and medicines to generate non-specific turbidity, so that the probability of a false positive detection result is reduced and the detection accuracy is relatively high.

Description

technical field [0001] The invention relates to a kit for detecting fungus 1,3-beta-D-glucan by chromogenic method of human body fluid. [0002] technical background [0003] In recent years, the prevalence of primary and secondary invasive fungal infections (IFIs) has continued to increase. As a nosocomial infection, IFI is very common in patients with malignant tumors, blood diseases receiving chemotherapy, AIDS patients, organ transplantation patients and patients receiving immunosuppressive therapy, resulting in serious complications and even death in more and more patients. Many fungal diseases are caused by inhalation of pollutants, plant debris, fungal spores in the air or on exposed surfaces, and some common fungi that are present on the human skin, intestinal tract and mucous membranes can also act as pathogenic bacteria to cause infection. At present, the diagnosis of invasive fungal infection and fungemia usually adopts non-specific diagnosis or radiological techn...

AI Technical Summary

Problems solved by technology

The kit can quickly and accurately quantify trace amounts of (1-3)-β-D-glucan in human blood and body fluid specimens. The chromogenic substance in this patent is Boc-LGR-pNA, and the After the reaction is terminated, the absorbance is detected by the endpoint method. This design has a long detection line and a high probability of introducing interference substances, which makes the detection time longer and the sensitivity is poor.

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