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A kind of nano drug loading system and its preparation method and application

A nano-drug loading and drug-loading nanotechnology, which is applied in the field of nano-drug loading system and its preparation, can solve the problems of poor biocompatibility, large toxic and side effects, large size, etc., and achieve improved inhibition ability, large tissue penetration, Effect of strong cancer site enrichment

Active Publication Date: 2018-02-02
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the size of the current dual-drug delivery system and photothermal therapy reagents are often relatively large, and it is difficult to control them within 100nm. Moreover, the commonly used photothermal therapy reagents are often gold nanorods, nanocages, carbon materials, biocompatible Poor sex, high toxicity and side effects

Method used

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  • A kind of nano drug loading system and its preparation method and application
  • A kind of nano drug loading system and its preparation method and application
  • A kind of nano drug loading system and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] In this example, the nano drug-carrying system is prepared by the following method, which specifically includes the following steps:

[0063] (1) 100mg PEG 2000 Disperse -DSPE in 5mL of methanol, stir to dissolve, add 10 mg of doxorubicin in dichloromethane solution (the mass ratio of PEG-DSPE to doxorubicin is 10:1), remove the organic solvent with a rotary evaporator to form a lipid film . Adding a tris-HCl buffer solution with a pH of 8.5 to the lipid film to form a drug-loaded nanomicelle solution, wherein the drug-loaded PEG-DSPE nanomicelle solution has a concentration of 2 mg / mL;

[0064] (2) Add 20 mg of dopamine hydrochloride to the drug-loaded PEG-DSPE nanomicelle solution obtained in step (1), and stir the reaction container for 24 hours to obtain a drug-loaded PEG-DSPE nanomicelle solution with a core-shell structure, 60000rmp Centrifuge to remove unreacted dopamine and buffer solution, freeze-dry for later use;

[0065] (3) Disperse the lyophilized drug-...

Embodiment 2

[0068] (1) 100mg PEG 2000-DSPE is dispersed in 6mL aqueous solution, ultrasonically, stirred, add 5mg of doxorubicin hydrochloride (the mass ratio of PEG-DSPE to doxorubicin hydrochloride is 20:1), stir slowly for 2h, then add tris-HCl buffer solution to adjust the pH value to 8.5, the concentration of the tris-HCl buffer solution is 10mmol / L, forming a drug-loaded nanomicelle solution, and making the concentration of the PEG-DSPE nanomicelles 15mg / mL;

[0069] (2) Add 150 mg of dopamine hydrochloride to the drug-loaded PEG-DSPE nanomicelle solution obtained in step (1), and stir and react for 14 hours under the condition that the opening of the reaction vessel is convected with the outside air to obtain drug-loaded PEG-DSPE with a core-shell structure. DSPE nano-micelle solution;

[0070] (3) Add the DMSO solution that is dissolved with 5mg bortezomib in the drug-loaded PEG-DSPE nano-micelle solution that step (2) obtains (the volume ratio of drug-loaded PEG-DSPE nano-micell...

Embodiment 3

[0073] (1) 100mg PEG 5000 -DSPE was dispersed in 10 mL of tris-HCl buffer solution (pH 8.0) with a concentration of 10 mmol / L to form a PEG-DSPE nano-micelle solution, and then 20 mg of doxorubicin hydrochloride was added and stirred to obtain a drug-loaded nano-micelle solution (loaded The concentration of drug nanomicelle is 10mg / mL);

[0074] (2) Add 10 mL of tris-HCl buffer solution (10 mmol / L, pH 8.0) that is dissolved with 100 mg of dopamine hydrochloride into the drug-loaded PEG-DSPE nanomicelle solution obtained in step (1), and the opening of the reaction vessel is connected to the outside world. Stir and react for 48 hours under air convection to obtain a drug-loaded PEG-DSPE nanomicelle solution with a core-shell structure, centrifuge at 60,000 rpm to remove unreacted dopamine and buffer solution, and freeze-dry for use;

[0075] (3) Add the lyophilized drug-loaded PEG-DSPE nanomicelles to the DMSO / water mixed solution (the volume ratio of water and DMSO is 8:1) di...

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Abstract

The invention provides a nanometer drug-loading system and its preparation method and application. The nanometer drug-loading system comprises a drug-loaded nanomicelle core, a polydopamine shell, and bortezomib connected to the polydopamine shell. The drug-loaded nanogel The beam is a nano-micelle formed by encapsulating chemical anticancer drugs in polyethylene glycol-distearoylphosphatidylethanolamine. The preparation method of the nanometer drug-carrying system of the present invention comprises the formation of drug-loaded nano micelles; forming a polydopamine shell outside the drug-loaded nano micelles; and connecting bortezomib to the polydopamine shell. The particle size of the nanometer drug loading system of the present invention is less than 50nm, has high stability, has tumor enrichment effect, and realizes simultaneous delivery of bortezomib and other chemical anticancer drugs. Polydopamine has photothermal effect, can assist chemotherapy drug treatment, and has The synergistic effect of combined chemotherapy and hyperthermia has broad medical application prospects.

Description

technical field [0001] The invention belongs to the field of nano-biomedicine, and relates to a nano-drug loading system and its preparation method and application. Background technique [0002] Breast cancer is a malignant tumor that seriously affects the health of women. It is mainly caused by the proliferation of malignant cells in the ductal epithelial cells of the mammary glands. Its etiology is not very clear, and it is currently believed that it may be related to changes in heredity, endocrine, viral infection, genes and cytokines. Surgery, radiotherapy, chemotherapy or endocrine therapy are the main treatment methods for breast cancer. However, these treatment methods are not effective, have large toxic and side effects, and poor patient compliance. Studies have shown that photothermal therapy is a low-invasive method that can improve the sensitivity of chemotherapy, and it is expected to cooperate with chemotherapy to improve the anti-tumor effect. [0003] As the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/69A61K38/05A61K31/704A61K33/24A61K41/00A61K47/24A61K47/34A61K45/00A61P35/00A61K31/337A61K31/675
Inventor 吴雁张瑞锐苏世帅邵磊厚
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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