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Polycyclic aromatic hydrocarbon removal composite material, and preparation method and use thereof

A technology of polycyclic aromatic hydrocarbons and composite materials, applied in the field of microbial engineering, can solve the problems of low abundance of functional microorganisms, difficulty in survival or short survival time, and influence on the removal effect of PAHs, and achieve good tolerance

Inactive Publication Date: 2015-11-11
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in PAHs-contaminated sites, the abundance of functional microorganisms with the ability to degrade PAHs is low, so screening and isolating PAHs-degrading bacteria from contaminated sites is one of the key technologies for the remediation of contaminated sites
[0003] At present, many scientific researchers have screened and isolated more PAHs-degrading bacteria from PAHs-contaminated sites, and adding functional microorganisms in the later stage to bio-augmented remediation has certain effects; however, free functional microorganisms are not easy to survive because they cannot adapt to the environment or The short survival time affects the removal effect of PAHs

Method used

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  • Polycyclic aromatic hydrocarbon removal composite material, and preparation method and use thereof
  • Polycyclic aromatic hydrocarbon removal composite material, and preparation method and use thereof
  • Polycyclic aromatic hydrocarbon removal composite material, and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: Screening and isolation of bacterial strains

[0065] Enrichment culture: Take PAH-contaminated soil of Beijing Coking Plant as the bacterial source, weigh 10g of soil, add 90ml of sterile water and 0.5g of glass beads, shake in a shaker at 180r / min, 30°C for 3h, and let it stand for 30min 5ml of the supernatant was inoculated into 45ml of sterilized MSM culture solution containing 50mg / L pyrene, cultured on a shaker at 30°C and 180r / min. Every 10 days, 5ml culture solution was transferred to 45ml pyrene concentration gradient to 100, 200, 300, 500mg / L fresh sterilized MSM culture solution, and the enrichment of PAH-degrading bacteria was completed after 5 transfers.

[0066] Screening of bacterial strains: Take a certain amount of the last enrichment culture solution and spread it on the MSM solid plate, turn the plate upside down in a beaker containing pyrene, and spread a layer of pyrene film on the MSM solid plate by sublimation method. The plate coated ...

Embodiment 2

[0073] Example 2: Substrate broad-spectrum test for the degradation of polycyclic aromatic hydrocarbons by bacterial strains

[0074] Add naphthalene, acenaphthene, dihydroacenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, and benzopyrene to a 100ml Erlenmeyer flask containing sterilized MSM culture solution, so that the contents of the above nine polycyclic aromatic hydrocarbons are all 50 mg / L, inoculate 0D with 10% inoculum 600 =1.0 Mycobacterium gilvum Bacterial liquid (gained in embodiment 1, hereinafter all the same). All the flasks were placed in a shaker at 30°C and 180r / min for shaking culture, and samples were taken on the 0th, 4th, and 8th days to determine the residual amounts of polycyclic aromatic hydrocarbons in the flasks. The experimental results show that the strains cannot degrade acenaphthene, dihydroacenaphthene, and benzopyrene in the above-mentioned polycyclic aromatic hydrocarbons. The degradation results of the other six kinds o...

Embodiment 3

[0079] Embodiment 3: Degradation test of bacterial strain to pyrene

[0080] Add a certain amount of pyrene (dissolved in acetone) into a 100ml Erlenmeyer flask containing sterilized MSM culture solution, and add MSM culture solution after the acetone has volatilized, so that the initial content of pyrene is 50mg / L. Inoculate 0D at 10% inoculum size 600 =1.0 Mycobacterium gilvum bacteria liquid. The flask was placed at 30°C and cultured in a shaker at 180r / min, and destructive sampling was carried out on days 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 to determine the residual pyrene in the flask. quantity. see results Figure 5 ,from Figure 5 It can be seen that the degradation rate of pyrene at an initial concentration of 50 mg / L by the strain can reach 57.85% on the 4th day, and can reach 96.76% on the 9th day.

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Abstract

The invention discloses a polycyclic aromatic hydrocarbon removal composite material. The effective component of the composite material comprises Mycobacterium gilvum with the preservation number of CGMCC No.10941. A preparation method of the composite material comprises the following steps: inoculating a seed solution of the cultured Mycobacterium gilvum (with the preservation number of CGMCC No.10941) to a container provided with charcoal, carrying out shake culturing on a shaking table, and carrying out immersion immobilization. The composite material can be used to remove or degrade polycyclic aromatic hydrocarbons in soil or water environment, and biologically restores polycyclic aromatic hydrocarbon and heavy metal combined polluted or polycyclic aromatic hydrocarbon individually-polluted soil or water environment.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a composite material for removing polycyclic aromatic hydrocarbons, a preparation method and an application thereof. Background technique [0002] In recent years, the pollution situation of PAHs in my country's soil is not optimistic. The average value of PAHs residues detected in the soil of different regions is much higher than that of other developed countries. Among them, high-molecular-weight PAHs pollutants with ring 4 and above account for more than 60% of the total. , this part of PAHs has a long half-life in soil, is highly toxic and difficult to degrade. At present, the remediation of PAH-contaminated sites mainly includes physical methods, chemical methods, biological methods and combined remediation. Among them, bioremediation has great application potential due to its advantages of low cost and no secondary pollution. A large number of studies have shown that in...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14C02F3/34B09C1/10C12R1/32C02F101/32
Inventor 蔡超熊毕景张又弛朱永官
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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