Polycyclic aromatic hydrocarbon removal composite material, and preparation method and use thereof

A technology of polycyclic aromatic hydrocarbons and composite materials, applied in the field of microbial engineering, can solve the problems of low abundance of functional microorganisms, difficulty in survival or short survival time, and influence on the removal effect of PAHs, and achieve good tolerance

Inactive Publication Date: 2015-11-11
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in PAHs-contaminated sites, the abundance of functional microorganisms with the ability to degrade PAHs is low, so screening and isolating PAHs-degrading bacteria from contaminated sites is one of the key technologies for the remediation of contaminated sites
[0003] At present, many scientific researchers have screened and isolat

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polycyclic aromatic hydrocarbon removal composite material, and preparation method and use thereof
  • Polycyclic aromatic hydrocarbon removal composite material, and preparation method and use thereof
  • Polycyclic aromatic hydrocarbon removal composite material, and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0064] Example 1: Screening and isolation of strains

[0065] Enrichment culture: Take the polycyclic aromatic hydrocarbon contaminated soil of Beijing Coking Plant as the source of bacteria, weigh 10g soil, add 90ml sterile water and 0.5g glass beads, shake in a shaker at 180r / min, 30℃ for 3h, and then stand for 30min. , Take 5ml of the supernatant and inoculate it into 45ml MSM culture medium containing 50mg / L of pyrene which has been sterilized, and culture at 30℃, 180r / min on a shaker. Transfer 5ml culture medium to 45ml pyrene concentration gradient into 100, 200, 300, 500 mg / L fresh sterilized MSM culture medium every 10 days, and complete the enrichment of PAH degrading bacteria after 5 transfers.

[0066] Screening of strains: After a certain amount of the last enrichment culture solution is applied to the MSM solid plate, the plate is inverted in a beaker containing pyrene, and a layer of pyrene film is spread on the MSM solid plate by sublimation. The plate coated with ...

Example Embodiment

[0073] Example 2: Broad-spectrum test of the strains degrading polycyclic aromatic hydrocarbons

[0074] Add naphthalene, acenaphthene, dihydroacenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, and benzopyrene into a 100ml Erlenmeyer flask containing sterilized MSM culture solution, so that the content of the above 9 polycyclic aromatic hydrocarbons is 50mg / L, inoculate 0D with 10% of the inoculation amount 600 =1.0 Mycobacteriumgilvum Bacteria liquid (obtained in Example 1, the same applies below). All the Erlenmeyer flasks were placed at 30°C and shaken in a 180r / min shaker. Samples were taken on the 0th, 4th, and 8th day to determine the residual amount of each polycyclic aromatic hydrocarbon in the Erlenmeyer flask. The experimental results show that the strain cannot degrade acenaphthene, dihydroacenaphthene and benzopyrene in the above-mentioned polycyclic aromatic hydrocarbons. The degradation results of the other 6 polycyclic aromatic hydrocarbons a...

Example Embodiment

[0079] Example 3: Degradation test of pyrene by strains

[0080] Add a certain amount of pyrene (dissolved in acetone) into a 100ml Erlenmeyer flask containing sterilized MSM culture solution. After the acetone volatilizes, add MSM culture solution to make the initial content of pyrene 50mg / L. Inoculate 0D at 10% of the inoculation volume 600 =1.0 Mycobacteriumgilvum Bacteria. Place the Erlenmeyer flask at 30°C and shake culture at 180r / min on a shaker. Destructively sample at 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 days to determine pyrene residue in the Erlenmeyer flask. the amount. See the result Figure 5 ,From Figure 5 It can be seen that the degradation rate of the strain to the initial concentration of 50 mg / L pyrene can reach 57.85% on the 4th day and 96.76% on the 9th day.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a polycyclic aromatic hydrocarbon removal composite material. The effective component of the composite material comprises Mycobacterium gilvum with the preservation number of CGMCC No.10941. A preparation method of the composite material comprises the following steps: inoculating a seed solution of the cultured Mycobacterium gilvum (with the preservation number of CGMCC No.10941) to a container provided with charcoal, carrying out shake culturing on a shaking table, and carrying out immersion immobilization. The composite material can be used to remove or degrade polycyclic aromatic hydrocarbons in soil or water environment, and biologically restores polycyclic aromatic hydrocarbon and heavy metal combined polluted or polycyclic aromatic hydrocarbon individually-polluted soil or water environment.

Description

technical field [0001] The invention relates to the field of microbial engineering, in particular to a composite material for removing polycyclic aromatic hydrocarbons, a preparation method and an application thereof. Background technique [0002] In recent years, the pollution situation of PAHs in my country's soil is not optimistic. The average value of PAHs residues detected in the soil of different regions is much higher than that of other developed countries. Among them, high-molecular-weight PAHs pollutants with ring 4 and above account for more than 60% of the total. , this part of PAHs has a long half-life in soil, is highly toxic and difficult to degrade. At present, the remediation of PAH-contaminated sites mainly includes physical methods, chemical methods, biological methods and combined remediation. Among them, bioremediation has great application potential due to its advantages of low cost and no secondary pollution. A large number of studies have shown that in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20C12N11/14C02F3/34B09C1/10C12R1/32C02F101/32
Inventor 蔡超熊毕景张又弛朱永官
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap