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Measuring method for detecting content of natural vitamins E in corn oil

The technology of a natural vitamin and a determination method is applied in the field of determination of the content of natural vitamin E in corn oil, which can solve the problems of complicated steps and long time, and achieve the effects of simple steps, good accuracy and precision, and high yield.

Inactive Publication Date: 2015-11-11
成都市新津迎先粮油有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These standards are extracted by saponification method before liquid chromatography analysis, which takes a long time and has complicated steps

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of assay method that detects natural vitamin E content in corn oil, comprises the following steps:

[0026] (1) Weighing: by weight, take 1 sample of corn oil and weigh it precisely;

[0027] (2) Ultrasonic extraction: Add 2~3 parts of diethyl ether-n-hexane mixed extractant (the mass ratio of diethyl ether to n-hexane is 2:5), ultrasonically extract for 20~25min, pour out the supernatant and collect;

[0028] (3) Repeat step (2) 2 times;

[0029] (4) Concentration: Collect the supernatant and concentrate to dryness to obtain the sample extract;

[0030] (5) Determination: Determination by high performance liquid chromatography, using C18 chromatographic column, the column temperature is controlled at 33°C, the volume ratio of mobile phase to water is 95:2, isocratic elution is 18min, the flow rate is controlled at 1.2mL / min, diode array The detection wavelength of the detector is 295nm, and the injection volume is 20μL;

[0031] Preparation of reference subst...

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Abstract

The invention relates to the field of natural product content measuring, in particular to a measuring method for detecting the content of natural vitamins E in corn oil. An ultrasonic method is adopted for optimizing the extracting condition of the natural vitamins E, a high-efficiency liquid chromatography method is adopted for measuring the content of the natural vitamins E in corn oil samples, and reference is provided for the vegetable oil quality standard. The steps are simple, and the method is economical, practical and suitable for popularization.

Description

technical field [0001] The invention relates to the field of content determination of natural products, in particular to a method for determining the content of natural vitamin E in corn oil. Background technique [0002] Natural vitamin E (VitaminE) is a fat-soluble vitamin, also known as tocopherol, is one of the most important antioxidants. Soluble in organic solvents such as fat and ethanol, insoluble in water, stable to heat and acid, unstable to alkali, sensitive to oxygen, not sensitive to heat, but the activity of vitamin E is significantly reduced when fried. Tocopherol can promote the secretion of sex hormones, increase the vitality and quantity of sperm in men; increase the concentration of estrogen in women, improve fertility, prevent miscarriage, and can also be used to prevent and treat male infertility, burns, frostbite, capillary bleeding, menopausal syndrome, Beauty and other aspects have a very good curative effect. In recent years, it has also been found...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/14
Inventor 高惠英
Owner 成都市新津迎先粮油有限公司