A kind of extraction method of high-purity rubusoside

Abstract

A high-purity rubusoside extraction method, comprising the following steps: (1) Pretreatment: removing impurities from dried sweet tea raw materials, crushing, and sieving to obtain sweet tea raw material powder; (2) Microwave countercurrent extraction: microwave sweet tea raw material powder Countercurrent extraction, extruding the extraction slag, mixing the extruded liquid with the sweet tea material liquid, filtering to obtain the extract; (3) centrifuging to obtain the centrifuge; (4) macroporous resin adsorption separation: adjust the pH of the centrifuge to 10.0~ 11.0, adsorbed by macroporous resin, washed with water after standing still, eluted, and collected the target eluent; (5) ultrafiltrated to obtain ultrafiltrate; (6) concentrated to obtain concentrated solution; (7) crystallization and recrystallization: to An organic solvent is added to the concentrated solution for crystallization, recrystallization and drying to obtain rubusoside. The rubusoside product obtained according to the method of the invention has a purity of ≥99% and a yield of ≥5.35%. The process of the invention is simple, the cycle is short, the energy consumption and production cost are low, continuous extraction is possible, and it is suitable for continuous production in factories.

Description

technical field [0001] The invention relates to a method for extracting rubusoside, in particular to a method for extracting high-purity rubusoside suitable for industrial production. Background technique [0002] Sweet tea is the sweet tea of ​​the genus Rubus of Rosaceae ( Rubus Suatrssimus S. Lee ) dry leaves, mainly distributed in the Dayao Mountains of Guangxi, and a small amount of planting in Guangdong, Hunan, Jiangxi and other places. It is sweet and has the effects of anti-pollen allergy, promoting insulin secretion, and preventing and treating cardiovascular diseases. Its main component is rubusoside, whose sweetness is about 300 times that of sucrose, and its calorific value is 5% of that of sucrose. It is mainly used as a sweetener , widely used in food, beverage and other industries, because of its remarkable characteristics of high sweetness and low calorific value, it is quite popular in the market. [0003] At present, the domestic research on extracting hi...

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Problems solved by technology

[0004] CN102702284A discloses a production method of high-purity rubusoside. The method is to extract, concentrate, flocculate and clarify sweet tea raw materials, centrifuge, and then undergo adsorption and separation of macroporous resins, ion resin exchange decolorization, desalination, compound decolorization, and concentrate The product is dried and prepared with a purity of 98%, but the yield is only 3.7-4.5%, and the process is complicated and the loss is large, resulting in a very low yield of the final product

[0005] CN102838644B discloses a production method for extracting rubusoside from sweet tea leaves. The method is to carry out ultrasonic-assisted extraction of sweet tea raw materials, alcohol precipitation and filtration after concentration under reduced pressure, adsorption and separation by macroporous resin, polyamide resin chromatography, crystallization And recrystallization to obtain the product, the product purity reaches more than 98%, and the yield is about 3.7-3.9%. This method has a low yield of the final product due to incomplete alcohol precipitation and incomplete elution of polyamide

[0006] CN104262425A discloses a new method for extracting rubusoside. The method is to carry out water extraction of sweet tea raw materials, and after membrane filtration, the product is obtained through macroporous resin adsorption separation, anion and cation resin separation, concentration, crystallization and recrystallization, and the product purity reaches 99%, and the yield is about 5.31%. Due to the repeated use of acid-base treatment during the adsorption and separation of macroporous resins, the subsequent treatment process is increased, the production cycle is prolonged, and higher requirements are placed on the equipment. The production cost Relatively high

[0007] CN104558088A discloses a method of using microwave countercurrent extraction, through multi-stage filtration, macroporous resin adsorption separation, membrane filtration, concentration, and drying, to obtain the process of mogroside V60% product, but in multi-stage filtration, use centrifugal It is carried out with multi-stage ultrafiltration, which increases the process, prolongs the filtration cycle, has low efficiency, and consumes a lot of energy. In addition, after purification by macroporous resin, it also contains a large amount of impurities. Nanofiltration is used to remove inorganic salts and solvents. , in order to achieve the purpose of concentration, the pressure in the process is high, the energy consumption is large, the damage to the membrane is also large, and the extraction efficiency and process cost are not good.