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Screening and application of Clostridium butyricum for balancing intestinal flora of livestock

A clostridium butyricum and intestinal technology, which is applied in the screening and application field of Clostridium butyricum, can solve problems such as the inhibitory effect of Clostridium butyricum intestinal pathogenic bacteria and the value-added effect of probiotics, so as to improve animal health. Effects on poultry performance

Active Publication Date: 2015-11-18
HUAZHONG AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

None of the above patent documents involves the verification of the inhibitory effect of Clostridium butyricum on intestinal pathogenic bacteria and the value-promoting effect on common probiotics in animal experiments

Method used

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  • Screening and application of Clostridium butyricum for balancing intestinal flora of livestock
  • Screening and application of Clostridium butyricum for balancing intestinal flora of livestock
  • Screening and application of Clostridium butyricum for balancing intestinal flora of livestock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation and identification of Clostridium butyricum HDRyYB1 bacterial strain

[0030] 1. Isolation of strains

[0031] (1) The biological material of the present invention collects the rectal contents of healthy goats from the farm of the Veterinary Hospital of Huazhong Agricultural University in Wuhan, Hubei, and divides the rectal contents samples into 6 groups, which are respectively placed in RCM liquid (purchased from BD Company of the United States) for cultivation. Put it in a water bath at 80°C for 10 minutes, cool it down and place it in an anaerobic incubator at 37°C for 18-24 hours, observe the gas production, pick out the gas production bottle and put it in a water bath at 80°C for 10 minutes, after cooling Transfer to RCM liquid medium with 2% (v / v) inoculum size and culture until gas production.

[0032] (2) Select the Erlenmeyer flask with more gas production, gradiently dilute the culture solution, and apply it to the flask added with CaCO ...

Embodiment 2

[0064] Example 2: Clostridium butyricum HDRyYB1 inhibits intestinal pathogenic bacteria and proliferates beneficial bacteria

[0065] With Salmonella choleraesuis C78-1 (purchased from China Veterinary Drug Control Institute), porcine pathogenic Escherichia coli O157 (preservation number ATCC35150, purchased from China Agricultural Microorganism Culture Collection Center), Staphylococcus aureus (ATCC25923, purchased from Sanitation Ministry of Clinical Inspection Center) as pathogen indicator bacteria, with Enterococcus faecium HDRsEf1 (patent publication number: CN102747003A) and bifidobacteria (purchased from China Industrial Microbiology Culture Collection Center, preservation number CICC6165, hereinafter referred to as bifidobacteria) these The medium bacterial strain is the indicator bacterium of beneficial bacterium (implementation of the present invention is not limited to above-mentioned bacterial strain, as long as it is similar bacterial strain and just can) carry out...

Embodiment 3

[0088] Example 3: Safety evaluation of Clostridium butyricum strain HDRyYB1

[0089] 1. Acute toxicity test

[0090] The maximum tolerated dose test was carried out according to the method of the national standard GB15193.3-2003 of the People's Republic of China.

[0091] The specific steps are as follows: 40 Kunming mice (purchased from Hubei Provincial Center for Disease Control and Prevention) with a body weight of 19g-22g were randomly divided into a test group and a control group, with 20 males and 20 mice each. After adapting to the environment for 5 days, the mice in the test group were given 0.5 mL of activated Clostridium butyricum HDRyYB1 bacterial solution of 1.6×109 CFU / mL, and the mice in the control group were given 0.5 mL of normal saline, and the mice were continuously observed for 8 days. The signs, poisoning performance and death of the mice were recorded during the experiment. At the end of the experiment, the mice were killed, the liver and spleen were ta...

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Abstract

The invention belongs to the technical field of preparation of veterinary microbial additives and particularly relates to screening and application of Clostridium butyricum for balancing intestinal flora of livestock and poultry. The Clostridium butyricum is characterized in that the Clostridium butyricum HDRyYB1 is isolated from intestinal contents of goats, and experiments show that the Clostridium butyricum has an evident function of inhibiting intestinal pathogenic bacteria and is capable of promoting proliferation of beneficial bacteria; the Clostridium butyricum is preserved in China Center for Type Culture Collection under CCTCC NO: M2015200. The Clostridium butyricum has the advantages of high growth speed, high stress resistance, safety, disease resistance, capability of improving growth performance and the like; the Clostridium butyricum is applicable to the preparation of microbial additives for livestock and poultry.

Description

technical field [0001] The invention belongs to the technical field of veterinary microbial additives, and in particular relates to the screening and application of a Clostridium butyricum that regulates the balance of intestinal flora of livestock and poultry. The invention relates to the application field of antibiotic additive technology. The invention relates to the isolation and identification, safety evaluation, stress resistance performance and probiotic performance identification of a sheep-derived probiotic Clostridium butyricum strain used for feeding, and its application as a feed additive for livestock and poultry. Background technique [0002] In animal husbandry production, antibiotics are widely used due to their advantages in preventing and controlling animal diseases and improving the production performance of livestock and poultry. Therefore, to a certain extent, the breeding industry has shifted from free-range farming to intensive and large-scale producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/145
Inventor 肖运才石德时毕丁仁刘锡玲王喜亮周祖涛张强
Owner HUAZHONG AGRI UNIV
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