Strain domesticating kit for degrading COD in coal-to-oil wastewater and domesticating method
A coal-to-oil and kit technology is applied to the domestication kit and the domestication field of bacteria for degrading the COD of coal-to-oil wastewater, which can solve the problems of refractory organic matter and long start-up time, so as to improve the adaptability and shorten the start-up time. Effect
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Embodiment 1
[0048] The kit formula and preparation method used in embodiment 1 are as follows:
[0049] 1. Enrichment tube:
[0050] Preparation of enrichment tubes:
[0051] 1. Take beef extract 1.0g, peptone 1.0g, potassium dihydrogen phosphate 2.0g, disodium hydrogen phosphate 2.5g, dissolve in 1L of water, and adjust the pH of the above medium to 7.00 with 5% sodium carbonate and 5% HCl. Dispense into 50ml screw tubes, add 20ml of culture medium to each tube; after dispensing, unscrew the cap of the screw tube, sterilize at 115°C for 25min, after the sterilization is completed and cooled to room temperature, remove from the sterilizer Take out and tighten the cap, and the preparation of the basic enrichment tube is completed;
[0052] 2. Take 13 mg of phenol, 4 mg of naphthol, 2 mg of quinoline, 0.12 ml of methanol, and 0.12 ml of butanol, dissolve them in 100 ml of sterile water, and filter them with a 0.22 μm sterile filter under aseptic conditions; After the sterilization is com...
Embodiment 2
[0089] The kit formula and preparation method used in embodiment 2 are as follows:
[0090] 1. Enrichment tube:
[0091] Preparation of enrichment tubes:
[0092] 1. Take 0.7g of beef extract, 0.7g of peptone, 1.2g of potassium dihydrogen phosphate, and 1.5g of disodium hydrogen phosphate, dissolve them in 1L of water, adjust the pH of the above medium to 7.50 with 5% sodium carbonate and 5% HCl, Dispense into 50ml screw tubes, add 20ml of culture medium to each tube; after dispensing, unscrew the cap of the screw tube, sterilize at 115°C for 30min, after the sterilization is completed and cooled to room temperature, remove from the sterilizer Take out and tighten the cap, and the preparation of the basic enrichment tube is completed;
[0093] 2. Take 10 mg of phenol, 2 mg of naphthol, 1 mg of quinoline, 0.08 ml of methanol, and 0.08 ml of butanol, and dissolve them in 100 ml of sterile water. After the sterilization is completed, take 2ml of the solution that has been filt...
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