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Polypeptide raw material prepared by enzymolysis of walnuts and application thereof

A walnut and enzymatic hydrolysis technology, applied in the field of biomedicine, can solve problems such as increasing process difficulty and protein denaturation, and achieve the effects of improving extraction rate, stabilizing glutamic acid content, and simplifying the process

Inactive Publication Date: 2015-11-18
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the meal contains a large amount of cellulose, and in the process of oil extraction, certain protein denaturation is caused, which greatly increases the difficulty of preparing memory-improving peptides using walnut protein.

Method used

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  • Polypeptide raw material prepared by enzymolysis of walnuts and application thereof
  • Polypeptide raw material prepared by enzymolysis of walnuts and application thereof
  • Polypeptide raw material prepared by enzymolysis of walnuts and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 Utilizes walnuts to prepare memory-improving peptides

[0072] (1) Add 10 parts of water to 1 part of walnut by weight, pass through a colloid mill to obtain walnut slurry, add 1mol / L NaOH solution to adjust the pH of the walnut slurry to 8.0, stir at 50°C for 60min, and then centrifuge at 3500r / min for 10min , filter to obtain supernatant; add walnut protein quality 1.0% (w / w) α-amylase and walnut quality 1.0% (w / w) cellulase to supernatant liquid, stir at 55 ℃ for 120min; Then add 1mol / L HCl solution to adjust the pH to 4.2, stir for 30min, and centrifuge at 3500r / min for 10min to obtain a precipitate; then stir with 45% ethanol, wash with water, and spray dry to obtain walnut protein powder.

[0073] (2) Add powdered walnut protein 10 times the water and stir evenly, add Novozymes’ Alcalase 2.4L at 2000U / g protein and Novozymes’ complex protease at 2500U / g protein, and hydrolyze at 55°C to The degree of hydrolysis is 12%, the enzyme is inactivated by heati...

Embodiment 2

[0078] Example 2 Using walnuts to prepare memory-improving peptides

[0079] (1) Add 15 parts of water to 1 part of walnut by weight, pass through a colloid mill to obtain walnut slurry, add 1mol / L NaOH solution to adjust the pH of the walnut slurry to 9.0, stir at 40°C for 90min, and then centrifuge at 3500r / min for 10min , filter to obtain the supernatant; add walnut protein quality 2.0% (w / w) α-amylase and walnut quality 0.5% (w / w) cellulase to the supernatant, and stir at 50° C. for 180 min; Then add 1mol / L HCl solution to adjust the pH to 4.2, stir for 60min, centrifuge at 3500r / min for 10min to obtain a precipitate; then stir with 60% ethanol, wash with water, and spray dry to obtain walnut protein powder.

[0080] (2) Add powdered walnut protein 7 times of water and stir evenly, add Novozymes’ Alcalase 2.4L at 3000U / g protein and Novozymes’ complex protease at 1200U / g protein, and hydrolyze at 50°C to The degree of hydrolysis is 8%, the enzyme is inactivated by heating...

Embodiment 3

[0085] Example 3 Using walnuts to prepare memory-improving peptides

[0086](1) Add 12 parts of water to 1 part of walnut by weight, pass through a colloid mill to obtain walnut slurry, add 1mol / L NaOH solution to adjust the pH of the walnut slurry to 8.5, stir at 45°C for 80min, and then centrifuge at 3500r / min for 10min , filter to obtain supernatant; add walnut protein quality 1.5% (w / w) α-amylase and walnut quality 0.8% (w / w) cellulase to supernatant liquid, stir 150min at 55 ℃; Then add 1mol / L HCl solution to adjust the pH to 4.2, stir for 45min, and centrifuge at 3500r / min for 10min to obtain a precipitate; then stir with 50% ethanol, wash with water, and spray dry to obtain walnut protein powder.

[0087] (2) Add powdered walnut protein 8 times the water and stir evenly, add Novozymes’ Alcalase 2.4L at 2500U / g protein and Novozymes’ complex protease at 1800U / g protein, and hydrolyze at 55°C to With a degree of hydrolysis of 10%, heat at 100° C. for 15 minutes to inacti...

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Abstract

The invention relates to the technical field of biological medicines and particularly relates to a polypeptide raw material prepared by enzymolysis of walnuts and an application thereof. The polypeptide raw material is characterized in that the combination of an alkaline-dissolving and acid-precipitating technology and a biological enzymolysis technology is adopted for extracting protein in the walnuts with high efficiency and degrading polysaccharide in alkaline-dissolving matters; multienzyme synergetic hydrolysis is adopted, the content of target amino acid with the function of improving the memory, such as serine and lysine and the like, in the polypeptide raw material is enriched. The polypeptide raw material and the application have the advantages that two-stage ultrafiltration membranes are adopted for separating walnut protein hydrolysates, on one hand, the polypeptide can be separated with high efficiency, the polypeptide with the molecular weight being between 1000-10000Da is enriched, simultaneously the purpose of concentrating the target polypeptide is also achieved, and the flow of the production process is simplified; the process operation is simple, the production cost is low, no any pollution is caused, the protein recovery rate is high, and the prepared polypeptide raw material is strong in activity, has the function of improving the memory action and can be widely applied in the field of foods.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a polypeptide raw material prepared by enzymatic hydrolysis of walnuts and its application. Background technique [0002] There are three causes of memory degradation: memory degradation caused by aging, memory loss caused by stress, etc., and memory damage caused by congenital or mechanical damage. Nowadays, social pressure is increasing and people's memory decline is becoming more and more prominent, which makes more scientific researchers focus on improving memory. [0003] At present, most scholars focus on the extraction and research of active substances in Chinese herbal medicines to improve memory. This method involves complicated processes and residues of organic reagents, which is easy to introduce safety problems. Another research hotspot is lecithin and polyunsaturated fatty acid deep-sea fish oil, etc. The raw materials of these substances are difficult to source...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30A23L1/29A61K38/01A61P25/00A23L33/00
Inventor 赵宏伟郭晓蕾马忠华苏国万赵谋明
Owner INFINITUS (CHINA) CO LTD
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