Injectable PLGA (poly(lactic-co-glycolic acid)) porous composite microsphere preparation embedded with BMP-2 and TGF-beta1 containing microspheres as well as preparation method and application of composite microsphere preparation

A technology of porous composite microspheres and BMP-2, which is applied in the field of medicine to reduce systemic side effects and improve curative effect

Inactive Publication Date: 2015-11-25
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The applicant found that there is currently no literature reporting a porous microsphere preparation that has a 3D porous structure that is conducive to the attachment and growth of seed cells, and can simultaneously release and control BMP-2 and TGF-β1, and can be used for injection

Method used

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  • Injectable PLGA (poly(lactic-co-glycolic acid)) porous composite microsphere preparation embedded with BMP-2 and TGF-beta1 containing microspheres as well as preparation method and application of composite microsphere preparation
  • Injectable PLGA (poly(lactic-co-glycolic acid)) porous composite microsphere preparation embedded with BMP-2 and TGF-beta1 containing microspheres as well as preparation method and application of composite microsphere preparation
  • Injectable PLGA (poly(lactic-co-glycolic acid)) porous composite microsphere preparation embedded with BMP-2 and TGF-beta1 containing microspheres as well as preparation method and application of composite microsphere preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Embodiment 1: preparation contains the polylactic acid glycolic acid ( Poly(lactic-co-glycolicacid, PLGA) porous composite microspheres

[0069] A, prepare chitosan microspheres containing BMP-2 or TGF-β1:

[0070] 根据文献报道(KimSE,ParkJH,ChoYW,ChungH,JeongSY,LeeEB,etal.Porouschitosanscaffoldcontainingmicrospheresloadedwithtransforminggrowthfactor-beta1:implicationsforcartilagetissueengineering.Journalofcontrolledrelease:officialjournaloftheControlledReleaseSociety.2003;91:365-74),采用如下方法制备:称取120mg壳聚糖(分子量 100000, Zhejiang Jinke Biochemical Co., Ltd.), add 3mL 2% (V / V) acetic acid aqueous solution, vortex to dissolve, add 1mL 1% (W / V) BMP-2 (U.S. Peprotech company) aqueous solution, mix After uniformity, drop in 90mL n-octanol (containing 4% Span 80, V / V), stir at 1200 rpm for 30 minutes, then add dropwise 10mL 5% (W / V) sodium tripolyphosphate (Sodiumtripolyphosphate, TPP, U.S. Amresco company) for cross-linking reaction, after continuing to stir for 1 hour, the chitosan mic...

Embodiment 2

[0074] Example 2: Morphological observation of PLGA porous composite microspheres embedded with BMP-2 and TGF-β1 chitosan microspheres

[0075] Get a certain amount of BMP-2 or TGF-β1 chitosan microspheres prepared in Example 1, and the PLGA porous composite microspheres containing chitosan microspheres, place on the sample plate that is glued with double-sided adhesive , observed under a scanning electron microscope (ZESSMA10, Germany) after gold plating, and obtained chitosan microspheres ( figure 2 A, the scale is 20 μm) and PLGA porous composite microspheres containing BMP-2 and TGF-β1 chitosan microspheres ( figure 2 B, morphology with scale bar 100 μm).

[0076] Chitosan microspheres are round in shape and smooth in surface, such as figure 2 As shown in A. The shape of PLGA porous microspheres is round, and a large number of pores are randomly distributed on the surface, such as figure 2 Shown in B.

Embodiment 3

[0077] Embodiment 3: the particle size determination of the PLGA porous composite microspheres embedded with BMP-2 and TGF-β1 chitosan microspheres

[0078] The PLGA porous composite microspheres containing BMP-2 or TGF-β1 chitosan microspheres inlaid prepared in Example 1 were dispersed in deionized water, and the particle diameter was measured with Malvern Masersizer2000 ( image 3 A). Randomly select 3 microspheres from the scanning electron microscope photos of porous microspheres, measure their surface pore size (negligible if the pore size is less than 5 μm), take the average value, and the pore size distribution diagram is as follows image 3 Shown in B. Measure the particle diameter of the microspheres on the scanning electron microscope photos containing BMP-2 or TGF-β1 chitosan microspheres, get the average value ( image 3 C).

[0079] Such as image 3 As shown in A, the average particle size of the PLGA porous composite microspheres embedded with BMP-2 and TGF-...

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Abstract

The invention belongs to the technical field of medicine, and provides a PLGA (poly(lactic-co-glycolic acid)) porous composite microsphere preparation embedded with BMP-2 and TGF-beta1 containing microspheres therein, which not only can bring about benefits for the adhesion and the growth of seed cells by virtue of a 3D porous structure but also can undergo controlled- and sustained-release and can be applied to injection. The invention also provides a preparation method of the porous composite microsphere preparation as well as application of the porous composite microsphere preparation in preparing bone or cartilage tissue defect repairing materials. The porous composite microsphere disclosed by the invention, which can serve as a good carrier for cell adhesion and proliferation and can be directly injected to a target part, can offer a possibility of non-surgical repair to bone and cartilage tissue injuries; and the porous composite microsphere preparation is extensive in clinical application and market prospect.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to an injectable PLGA porous composite microsphere preparation embedded with BMP-2 and TGF-β1 microparticles, a preparation method and application thereof. Background technique [0002] Bone and cartilage injuries often occur in daily life, and the clinical treatment effect is not good. Conventional surgical treatment often brings continuous pain to patients and easily leads to side effects such as nonunion. Using bone tissue engineering, the use of degradable polymer materials to make tissue engineering scaffolds to repair bone and cartilage damage is a research hotspot in recent years. Tissue engineering is a discipline that applies the principles and technologies of life science and engineering to study biological substitutes for repairing, maintaining and promoting the shape and function of human tissues or organs after damage. With the need of non-invasive and mini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/54A61L27/56A61L27/58
Inventor 陈琰钟延强陶春鲁莹邹豪
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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