Method of utilizing acidic mannase to hydrolyze refined konjac powder to prepare konjac oligosaccharide
A technology of mannanase and konjac fine powder, which is applied in the field of food processing, can solve the problems of negative impact on animal growth and production performance, inability to utilize nutrients, and affect the absorption of nutrients, etc., and achieve good health care effects, mild conditions, and simple low sugar effect
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[0031] Example 1
[0032] A method for preparing konjac oligosaccharide by utilizing acid mannanase to hydrolyze konjac refined powder, comprising the following steps:
[0033] (1) Preparation of enzyme solution:
[0034] Add deionized water to the reaction kettle in advance, adjust the pH value of the water to 4.5 with hydrochloric acid, heat to 40°C, prepare an aqueous solution of β-mannanase according to the ratio of 200IU / g konjac powder, stir and mix evenly;
[0035] (2) Hydrolysis of konjac flour:
[0036]Turn on low-speed stirring, slowly add konjac flour into the aqueous solution of β-mannanase, as the konjac flour in the reactor absorbs water and expands and the viscosity increases, the addition of konjac flour can be stopped appropriately. Once it becomes thinner, continue to add konjac flour. , the weight required to add konjac flour accounts for 15% (mass concentration) of the entire reaction system, and the operation requires that the entire feeding time is with...
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[0043] Example 2
[0044] A method for preparing konjac oligosaccharide by utilizing acid mannanase to hydrolyze konjac refined powder, comprising the following steps:
[0045] (1) Preparation of enzyme solution:
[0046] Add deionized water to the reaction kettle in advance, adjust the pH value of the water to 7.0 with acid, heat to 60°C, configure the β-mannanase aqueous solution according to the ratio of 300IU / g konjac powder, stir and mix evenly;
[0047] (2) Hydrolysis of konjac flour:
[0048] Turn on low-speed stirring, slowly add konjac flour into the aqueous solution of β-mannanase, as the konjac flour in the reactor absorbs water and expands and the viscosity increases, the addition of konjac flour can be stopped appropriately. Once it becomes thinner, continue to add konjac flour. , the weight required to add konjac flour accounts for 25% (mass concentration) of the entire reaction system, and the operation requires that the entire feeding time is within 20min, th...
Example Embodiment
[0055] Example 3
[0056] A method for preparing konjac oligosaccharide by utilizing acid mannanase to hydrolyze konjac refined powder, comprising the following steps:
[0057] (1) Preparation of enzyme solution:
[0058] Add deionized water to the reaction kettle in advance, adjust the pH of the water to 5.5 with acid, heat to 50°C, configure the β-mannanase aqueous solution according to the ratio of 250IU / g konjac powder, stir and mix well;
[0059] (2) Hydrolysis of konjac flour:
[0060] Turn on low-speed stirring, slowly add konjac flour into the aqueous solution of β-mannanase, as the konjac flour in the reactor absorbs water and expands and the viscosity increases, the addition of konjac flour can be stopped appropriately. Once it becomes thinner, continue to add konjac flour. , the weight required to add konjac flour accounts for 20% (mass concentration) of the entire reaction system, and the operation requires that the entire feeding time is within 20min, the enzyma...
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