Preparation and application of nano diamond drug with high load and pH capable of controlling release of adriamycin
A nano-diamond and doxorubicin technology, used in drug combinations, medical preparations with inactive ingredients, anti-tumor drugs, etc., can solve the problems of strong cytotoxicity, lack of specific recognition, killing, etc., and achieve stable chemical properties. , good biocompatibility, reducing the effect of toxic and side effects
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Embodiment 1
[0036] (1) Preparation of nanodiamond-polyethylene glycol (ND-PEG-COOH) nanoparticles
[0037] Accurately weigh 1.0 mg of carboxylated nano-diamonds, and pass through dimethyl sulfoxide (DMSO), absolute ethanol (C 2 h 5 OH), distilled water (H 2 O) After washing, place in 1.0mL MES (0.1M, pH5.8) buffer solution, ultrasonically disperse for 30min to form a suspension, then add 0.2mgEDC, 0.25mgNHS, stir at room temperature for 6h, and centrifuge at 15000rpm after the reaction is complete 5min, remove supernatant, obtain the nano-diamond precipitate of activated carboxyl group;
[0038] Quickly disperse the nano-diamond precipitate with activated carboxyl group into 1.0mL BBS (0.1M, pH8.4) buffer solution, ultrasonically disperse for 10min to form a suspension, then add 0.3mg polyethylene glycol (H 2 N-PEG-COOH), continue to stir and react at room temperature for 12 hours. After the reaction is completed, collect the supernatant by high-speed centrifugation at 15,000 rpm for 5...
Embodiment 2
[0043] (1) The preparation of nano diamond-polyethylene glycol (ND-PEG-COOH) nanoparticles is the same as in Example 1
[0044] (2) Nano diamond-polyethylene glycol-doxorubicin / sodium citrate (ND-PEG-DOX / Na 3 Cit) Preparation of Nanomedicine
[0045] Weigh 1 mg of vacuum-dried nano-diamond-polyethylene glycol (ND-PEG-COOH), add 1 mL of sodium citrate (Na 3 Cit, 1.0M) solution, ultrasonically disperse for 1 hour to form a suspension, then add 0.1 mg doxorubicin (DOX), and react on a shaker in the dark for 6 hours. After the reaction is complete, centrifuge at 15,000 rpm for 5 minutes to collect the supernatant solution, and with 1.0M sodium citrate (Na 3 Cit) continue to wash three times with 15000rpm high-speed centrifugation for 5min to obtain nanodiamond-polyethylene glycol-doxorubicin / sodium citrate (ND-PEG-DOX / Na 3 Cit) nanoparticles, placed in a vacuum oven to preserve for subsequent use. All supernatants were collected into a small beaker and left overnight in a refr...
Embodiment 3
[0047] (1) With embodiment 1 step (1).
[0048] (2) Preparation of nanodiamond-polyethylene glycol-doxorubicin / sodium acetate (ND-PEG-DOX / NaAc) nanomedicine
[0049] Weigh 1 mg of vacuum-dried nano-diamond-polyethylene glycol (ND-PEG-COOH), add 1 mL of sodium acetate (NaAc, 1.0 M) solution, and ultrasonically disperse for 1 hour to form a suspension, then add 0.2 mg of doxorubicin ( DOX), avoid light and react on a shaker for 6 hours. After the reaction is completed, collect the supernatant with 15000rpm high-speed centrifugation for 5min, and continue to wash three times with 1.0M sodium acetate (NaAc) with 15000rpm high-speed centrifugation for 5min to obtain nano-diamond-polymer Ethylene glycol-doxorubicin / sodium citrate (ND-PEG-DOX / NaAc) nano-medicine is stored in a vacuum drying oven for future use. All supernatants were collected into a small beaker and left overnight in a refrigerator at 4°C to determine the mass of DOX adsorbed on ND-PEG-COOH nanoparticles. The absor...
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