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Method for detecting content of various components in vitamin B compound

A compound vitamin and detection method technology, which is applied in the field of detection of the content of multiple components in compound vitamin B, can solve the problems of complex processing, long detection time, and inability to detect simultaneously, achieving good separation and good reproducibility Effect

Inactive Publication Date: 2015-12-02
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Among the existing detection standards, the detection of vitamin B 1 , Vitamin B 2 , Vitamin B 6 The national standards for niacinamide, pantothenic acid, etc. must be tested separately, and cannot be tested at the same time, resulting in long testing time and complex problems

Method used

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  • Method for detecting content of various components in vitamin B compound
  • Method for detecting content of various components in vitamin B compound
  • Method for detecting content of various components in vitamin B compound

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0052] The content detection of multiple components in the compound vitamin B of embodiment 1

[0053] Detected ingredients: pantothenic acid, niacinamide, vitamin B 6 , Vitamin B 2 , Vitamin B 1 ;

[0054] 1. Chromatographic conditions

[0055] Chromatographic column: C18 column, 150mm×4.6mm, 5μm;

[0056] Mobile phase: organic phase is acetonitrile;

[0057] The water phase is 1.0g sodium heptanesulfonate and 1mL phosphoric acid, add water to 1000mL;

[0058] Gradient elution program: 0-15min, acetonitrile rises from 5% to 23%, after 15min, acetonitrile drops to 5%, and keeps for 5min;

[0059] High performance liquid chromatography (with UV detector and diode array detector) detection wavelength: 200nm and 280nm;

[0060] Flow rate: 1.0mL / min;

[0061] Column temperature: 40°C.

[0062] 2. Drawing of the standard curve: Precisely absorb the stock solution of the reference substance to make five working standard solutions of the control concentration, filter through...

Embodiment 2

[0071] The content detection of multiple components in the compound vitamin B of embodiment 2

[0072] Detected ingredients: pantothenic acid, niacinamide, vitamin B 6 , Vitamin B 2 , Vitamin B 1 ;

[0073] 1. Chromatographic conditions

[0074] Chromatographic column: C18 column, 150mm×4.6mm, 5μm;

[0075] Mobile phase: organic phase is acetonitrile;

[0076] The water phase is 1.0g sodium heptanesulfonate and 1mL phosphoric acid, add water to 1000mL;

[0077] Gradient elution program: 0-15min, acetonitrile rises from 4% to 22%, after 15min, acetonitrile drops to 4%, and keeps for 3min;

[0078] High performance liquid chromatography (with UV detector and diode array detector) detection wavelength: 200nm and 280nm;

[0079] Flow rate: 0.9mL / min;

[0080] Column temperature: 38°C.

[0081] 2. Drawing of the standard curve: Precisely absorb the stock solution of the reference substance to make five working standard solutions of the control concentration, filter through...

Embodiment 3

[0090] The content detection of multiple components in the compound vitamin B of embodiment 3

[0091] Detected ingredients: pantothenic acid, niacinamide, vitamin B 6 , Vitamin B 2 , Vitamin B 1 ;

[0092] 1. Chromatographic conditions

[0093] Chromatographic column: C18 column, 150mm×4.6mm, 5μm;

[0094] Mobile phase: organic phase is acetonitrile;

[0095] The water phase is 1.0g sodium heptanesulfonate and 1mL phosphoric acid, add water to 1000mL;

[0096] Gradient elution program: 0-15min, acetonitrile rises from 6% to 24%, after 15min, acetonitrile drops to 6%, and keeps for 10min;

[0097] High performance liquid chromatography (with UV detector and diode array detector) detection wavelength: 200nm and 280nm;

[0098] Flow rate: 0.8mL / min;

[0099] Column temperature: 35°C.

[0100] 2. Drawing of the standard curve: Precisely absorb the stock solution of the reference substance to make five working standard solutions of the control concentration, filter throug...

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Abstract

The invention relates to the technical field of analytical chemistry, in particular to a method for detecting the content of various components in a vitamin B compound. The detection method includes the following steps that the vitamin B compound is collected to enable a test solution and a standard product solution to be subjected to HPLC detection, and the chromatographic conditions for HPLC detection are that a C18 chromatographic column is adopted, acetonitrile is used as a mobile phase organic phase, and an aqueous solution of sodium heptanesulfonate and phosphoric acid is used as a mobile phase aqueous phase for gradient elution; in accordance with the HPLC detection result, the content of pantothenic acid, nicotinamide, vitamin B6, vitamin B2 and vitamin B1 is acquired. By the adoption of the method, the content of the vitamin B can be detected quickly and accurately. The method is good in linear relation, stability and accuracy, low in limit of detection and high in recovery rate.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a method for detecting the contents of multiple components in complex vitamin B. Background technique [0002] Vitamins are a class of organic compounds necessary to maintain human health, promote growth and development, and regulate physiological functions. These compounds are all present in natural food, and generally cannot be synthesized in the body or the amount of synthesis is small, which cannot meet the needs. Due to dietary habits, lack of nutritional knowledge, and changes in modern lifestyles, it is difficult to achieve an ideal balanced diet pattern. According to authoritative department surveys, most people in China cannot provide certain vitamins and minerals in a balanced diet, lacking calcium, iron, and vitamin A. , B 2 More common, lack of zinc, selenium, vitamin B 1 , vitamin C is also very common. Therefore it is necessary to take nutritional s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 蔡伟江陈彩云
Owner BY HEALTH CO LTD
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