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Composition, stabilizer and use thereof, thrombin preservation method, preparation method, thrombin preparation, kit

A preservation method and thrombin technology, which can be used in drug combinations, non-active components of polymer compounds, and medical preparations with non-active components, etc., and can solve the problems of reduced enzyme activity, poor stability, and inconvenience for patients to use.

Active Publication Date: 2018-12-21
BEIJING MDC NEW SPRING MEDICAL DEVICES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This has the following problems: first, it is difficult to preserve at room temperature, which will reduce the enzyme activity; secondly, it is inconvenient for patients to use
However, thrombin, like most enzymes, has poor stability, and its aqueous solution will be completely inactivated after being stored at room temperature for 24 days. If no protective agent or stabilizer is added, or its formula is not suitable, thrombin is used as a hemostatic agent Or make diagnostic reagents, its product quality is all difficult to guarantee, so it is necessary to develop a kind of stabilizer, to enhance the stability of thrombin, thereby improve the stability of product quality with thrombin as the main component

Method used

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  • Composition, stabilizer and use thereof, thrombin preservation method, preparation method, thrombin preparation, kit
  • Composition, stabilizer and use thereof, thrombin preservation method, preparation method, thrombin preparation, kit
  • Composition, stabilizer and use thereof, thrombin preservation method, preparation method, thrombin preparation, kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The preparation of embodiment 1 composition

[0068] Accurately weigh 40g of trehalose, 5g of glycine, 5g of sodium chloride, 5g of bovine serum albumin, 1g of sodium azide and 0.1g of sodium thiosulfate, and mix them.

Embodiment 2

[0069] The preparation of embodiment 2 compositions

[0070] Accurately weigh 70g of trehalose, 15g of glycine, 7.5g of sodium chloride, 7.5g of bovine serum albumin, 3g of sodium azide and 0.15g of sodium thiosulfate, and mix them.

Embodiment 3

[0071] The preparation of embodiment 3 compositions

[0072] Accurately weigh 80 g of trehalose, 10 g of glycine, 8 g of sodium chloride, 6 g of bovine serum albumin, 4 g of sodium azide and 0.1 g of sodium thiosulfate, and mix them.

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Abstract

The invention relates to the field of biological technology, in particular to composition, a stabilizer, an application of the stabilizer, preservation and preparation methods of thrombin, a thrombin preparation and a kit. The composition and the stabilizer can guarantee that the enzyme activity of liquid thrombin is not lost under a room temperature condition in two years. Besides, the liquid thrombin prepared with the method can be used for preparing a thrombin hemostatic and manufacturing a kit for testing the content of FIB (fibrinogen), has wide and profound market prospect and can create considerable economic benefits and good social benefits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a composition, a stabilizer and uses thereof, a thrombin preservation method, a preparation method, a thrombin preparation, and a kit. Background technique [0002] Thrombin, a proteolytic enzyme formed by activation of thrombin precursor (an essential component in plasma), can catalyze fibrinogen into fibrin to promote blood coagulation, and is used for local hemostasis of capillary bleeding and surgical operations Post tissue healing. [0003] Thrombin can directly act on fibrinogen in the blood to promote its transformation into fibrin. After local application, the blood acting on the surface of the lesion quickly forms a stable clot, which is used to control capillary and venous bleeding, or as an adhesive and fixative for skin and tissue grafts. Other effects of thrombin on the blood coagulation system include the induction of platelet aggregation and secondary release reactio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K47/36A61K47/18A61K47/02A61K38/48A61P7/04
Inventor 胡军胡琦杨军艳
Owner BEIJING MDC NEW SPRING MEDICAL DEVICES