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Method for preparing Yunnan pine bark procyanidine

A bark proanthocyanidin and proanthocyanidin technology, which is applied in the field of extraction and purification of Yunnan pine bark proanthocyanidin preparation, can solve the problems of insufficient extraction efficiency and single means, and achieve the effects of simple and easy operation, accelerated mass transfer process, and reduced cost

Active Publication Date: 2015-12-23
北京中农弘科生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method used is relatively simple, and the extraction efficiency is still insufficient.

Method used

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  • Method for preparing Yunnan pine bark procyanidine
  • Method for preparing Yunnan pine bark procyanidine
  • Method for preparing Yunnan pine bark procyanidine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1, the influence of different enzymes on the extraction rate of proanthocyanidins from Yunnan pine bark

[0067] 1) Pulverize the bark of pine bark after cleaning and removing impurities, pass through a 60-mesh sieve, and add normal temperature water as an extraction solvent. The enzyme is one of acid protease (pepsin), cellulase or pectinase, and 1g of acid protease with enzyme activity of 1500U / mg or 1g of enzyme activity of 0.8 U / mg of cellulase or 1g of pectinase with an enzyme activity of 1.0U / mg. The pH of the mixed solution after adding the enzyme was adjusted to 3.5, and placed in a water bath constant temperature oscillator at 45° C. for 120 min to obtain an enzymatic hydrolysis solution. No enzyme was added to the blank control group, and other conditions remained unchanged.

[0068] 2) Centrifuge the mixed solution after enzymolysis in step 1) at a centrifugal force of 4000g-6000g (specifically 5000g) for 10min-30min (specifically 15min) to obtai...

Embodiment 2

[0073] Embodiment 2, the influence of enzymolysis conditions on the extraction rate of proanthocyanidins from Yunnan pine bark

[0074] 1) Crush the bark of pine bark after cleaning and removing impurities, pass through a 60-mesh sieve, add room temperature water as an extraction solvent, and then add enzymes, add 1 g of enzymes to every 100 g of raw materials (crushed pine bark), the activity of which is 1.0 U / mg of pectinase, carry out enzymatic hydrolysis under certain conditions to obtain enzymatic hydrolyzate. According to the author's research, four factors, such as solid-liquid ratio, enzymatic hydrolysis pH, enzymatic hydrolysis temperature and enzymatic hydrolysis time, which have a great influence on the extraction rate of proanthocyanidins were selected for L 9 (3 4 ) orthogonal experiment, the designed four-factor three-level code is shown in Table 2, that is: the solid-liquid ratio of crushed Yunnan pine bark and normal temperature water is 1g: 5mL, 1g: 10mL or ...

Embodiment 3

[0082] Embodiment 3, the impact of high-voltage pulsed electric field treatment pulse number on the extraction rate of proanthocyanidins from Yunnan pine bark

[0083] 1) Crush the bark of Pine yunnanensis after cleaning and removing impurities, pass through a 60-mesh sieve, add normal temperature water as the extraction solvent, the ratio of solid to liquid between the bark of pine yunnanensis after crushing and clean water at normal temperature is 1g: 10mL, and then add enzyme, every 100g Add 1 g of pectinase with an enzyme activity of 1.0 U / mg to the raw material (crushed Yunnan pine bark). The pH of the mixed solution after adding the enzyme was adjusted to 3.5, and placed in a water bath constant temperature oscillator at 55° C. for 60 min to obtain an enzymatic solution.

[0084] 2) Homogenize the enzymolysis solution obtained in step 1) with a rubber mill for 15min-30min (specifically 20min), pump the mixed solution after the homogenized rubber mill into a high-voltage ...

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Abstract

The invention discloses a method for preparing Yunnan pine bark procyanidine. The method comprises steps: firstly, Yunnan pine barks are crushed and then sieved, enzymolysis auxiliary digestion is carried out in a solvent, and an enzymatic hydrolysate is obtained; secondly, the enzymatic hydrolysate obtained from the first step is subjected to colloid milling, homogenizing, and high-voltage pulse electric field processing, the obtained product is centrifuged, the supernatant liquid is collected and condensed, and a procyanidine crude extracting solution is obtained; thirdly, the procyanidine crude extracting solution obtained from the second step is purified and dried, and a finished product is obtained. The method is advantaged by simple technology and mild conditions. The selected Yunnan pine bark raw material is low in price and has abundant sources, the production cost can be lowered greatly, and the development prospect is wide. Through the enzyme-high voltage pulse electric field auxiliary extraction method, the extraction rate and the yield of Yunnan pine bark procyanidine are raised, and the extraction time is shortened. Organic solvents of methanol, ethanol and the like are not used, resources are saved, and pollution discharge is reduced. The extraction rate of the prepared Yunnan pine bark procyanidine is more than or equal to 95%, the purity is more than or equal to 75% and the yield is more than or equal to 1.0%.

Description

technical field [0001] The invention belongs to the technical field of separation of plant extracts, and in particular relates to a preparation method for extracting and purifying proanthocyanidins from bark of Yunnan pine. Background technique [0002] We know that with the improvement of modern living standards and quality, people's health awareness is also increasing, proanthocyanidins with excellent physiological properties and functions have become a hot spot of concern. Because of its antioxidant, anti-inflammatory, antibacterial, anti-aging, anti-cancer and protective effects on cardiovascular, heart and vision, proanthocyanidins, as important health care products and therapeutic drugs, can be used to improve blood circulation and treat diabetic retina disease, reduce edema and inhibit varicose veins, etc. At the same time, proanthocyanidins also play an important role in animal husbandry as feed additives. Previous studies have shown that diarrhea in weaned piglets...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 刘明
Owner 北京中农弘科生物技术有限公司
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