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Nosiheptide finemeal extracting method

An extraction method, nosiheptide technology, applied in the field of veterinary drug preparation, can solve problems such as unsuitability for industrial production, difficulty in extraction work, and reduced product yield, and achieve the effects of reducing waste water pollution, reducing production costs, and large processing capacity

Inactive Publication Date: 2015-12-30
ZHEJIANG ESIGMA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: 1. The concentration temperature of n-butanol extract is high, and nosiheptide is unstable at high temperature, so it brings great difficulties to the extraction work; 2. A large amount of emulsification is easily produced during n-butanol extraction layer, the separation of the emulsified layer greatly reduces the yield of the product (crude product 70%, fine product 50%), so it is not suitable for industrialized production

Method used

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  • Nosiheptide finemeal extracting method
  • Nosiheptide finemeal extracting method
  • Nosiheptide finemeal extracting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Get inactivated nosiheptide fermentation broth 3000g, add diatomaceous earth 30g, adopt horizontal spiral sedimentation centrifuge centrifugal treatment, obtain the filter residue (containing water) of 120g containing nosiheptide;

[0023] (2) Take the nosiheptide filter residue obtained in step (1), add 360g dichloroethane solvent therein, and stir at 45°C for 0.5 hour, filter, separate layers, take the organic phase, and obtain 364g nosiheptide extract and 116g Mycelium filter residue;

[0024] (3) Take the local mycelium filter residue obtained in step (2), add 116g of dichloroethane therein, and stir at 45°C for 0.5 hours, filter, separate layers, take the organic phase, and obtain 117.5g of nosiheptide extract;

[0025] (4) Combine step (2) and (3) obtained cetide extracts, distill under reduced pressure to dryness, add deionized water 481.5g deionized water to it at room temperature, filter, filter cake is washed with water, dried, ground to obtain 5.42g nosi...

Embodiment 2

[0030] (1) Get inactivated nosiheptide fermentation broth 3000g, add diatomaceous earth 30g, adopt horizontal spiral sedimentation centrifuge centrifugal treatment, obtain the filter residue (containing water) of 120g containing nosiheptide;

[0031] (2) Take the nosiheptide filter residue obtained in step (1), add 480g dichloroethane solvent therein, and stir at 48°C for 0.8 hours, filter, separate layers, take the organic phase, and obtain 512g nosiheptide extract and 88g Mycelium filter residue;

[0032] (3) Take the local mycelium filter residue obtained in step (2), add 132g dichloroethane therein, and stir at 48°C for 0.8 hours, filter, separate layers, take the organic phase, and obtain 122g nosiheptide extract;

[0033] (4) Combine step (2), (3) obtained cetide extracts, distill under reduced pressure to dryness, add deionized water 698g deionized water to it at room temperature, filter, filter cake is washed with water, dried, ground to obtain 5.86 g nosiheptide powd...

Embodiment 3

[0038] (1) Get inactivated nosiheptide fermentation broth 3000g, add diatomaceous earth 30g, adopt horizontal spiral sedimentation centrifuge centrifugal treatment, obtain the filter residue (containing water) of 120g containing nosiheptide;

[0039] (2) Take the nosiheptide filter residue obtained in step (1), add 600g dichloroethane solvent therein, and stir for 1 hour at 50°C, filter, separate layers, take the organic phase, and obtain 615g nosiheptide extract and 105g Mycelium filter residue;

[0040] (3) Take the mycelium filter residue obtained in step (2), add 210g of dichloroethane therein, and stir at 50°C for 1 hour, filter, separate layers, take the organic phase, and obtain 223g of nosiheptide extract;

[0041] (4) Combine the cetide extracts obtained in steps (2) and (3), distill to dryness under reduced pressure, add 1257g of deionized water to it at room temperature, filter, and wash the filter cake with water, dry and grind to obtain 5.79g of cetide Peptide po...

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Abstract

The invention provides a nosiheptide finemeal extracting method. The nosiheptide finemeal extracting method comprises the following steps of adding a filter aid in inactivated nosiheptide fermentation liquor; performing centrifugal treatment to obtain filter residues; adding dichloroethane in the filter residues; stirring for 0.5-1 hour at the temperature of 45-50 DEG C; filtering; keeping the filtrate standing until the filtrate is layered; taking an organic phase to obtain a nosiheptide extracting solution; performing reduced pressure distillation to recycle dichloroethane; adding deionized water at a room temperature; filtering; washing filter cakes with water and drying the washed filter cakes; and grinding the filter cakes to obtain nosiheptide finemeal. The distilled out dichloroethane can be directly recycled and used. The potency of the nosiheptide finemeal obtained by the method is 970 ug / mg, products do not contain mycelium, and yield reaches up to 93%. A nosiheptide extracting technology is simple, extracting efficiency is high, a dichloroethane solvent can be recycled and reused, so that wastewater pollution is reduced, production cost is reduced, and nosiheptide finemeal is suitable to be produced industrially.

Description

technical field [0001] The invention belongs to the technical field of veterinary drug preparation, and in particular relates to a method for extracting nosiheptide fine powder. Background technique [0002] Nosipeptide, also known as nosipeptide, nopeptidemycin and nopeptidemycin, is a sulfur-containing polypeptide antibiotic with the molecular formula C 51 h 43 N 13 o 12 S 6 . It is produced by the fermentation of actinomycete strains. It is light yellow to light brown crystalline powder, insoluble in water and petroleum ether, slightly soluble in methanol, ethanol, ethyl acetate and benzene, soluble in chloroform, pyridine, dimethyl Formamide has a melting point of 310-320°C (with decomposition). The mechanism of action of nosiheptide is to hinder the protein synthesis of bacteria, and it has antibacterial effect at low concentration and bactericidal effect at high concentration. Because nosiheptide inhibits the growth of harmful bacteria in the intestines, the abs...

Claims

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Application Information

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IPC IPC(8): C07K5/078C07K1/36C07K1/34C07K1/30
Inventor 吴中华张小朋陈贵才徐天华何奇雷闻鸣
Owner ZHEJIANG ESIGMA BIOTECH CO LTD
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