Bacillus subtilis for controlling cotton rhizoctonia and application of bacillus subtilis

A technology for Bacillus subtilis and cotton blight, which is applied in the directions of chemicals for biological control, applications, microorganism-based methods, etc., and can solve the problem of no reports of Bacillus subtilis strain HMB27703 being found.

Inactive Publication Date: 2015-12-30
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After retrieval, there is no report on the prevention and treatment of cot

Method used

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  • Bacillus subtilis for controlling cotton rhizoctonia and application of bacillus subtilis
  • Bacillus subtilis for controlling cotton rhizoctonia and application of bacillus subtilis
  • Bacillus subtilis for controlling cotton rhizoctonia and application of bacillus subtilis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation of embodiment 1HMB27703 microbial bacterial agent

[0057] Follow the steps below:

[0058] (1) Strain activation: the strain HMB27703 stored at -80°C (the Bacillus subtilis strain HMB27703 has been preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on September 29, 2015, and the preservation number is CGMCCNo.11458) Activate on LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) at 30°C, pick a single colony on the LB slope culture medium (its composition and weight ratio are: 10g of tryptone, 5g of yeast extract, 5g of sodium chloride, 15g of agar powder, and 1000mL of water) at 30°C for 12 hours to obtain an activated strain;

[0059] (2) Preparation of seed liquid: make LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g...

Embodiment 2

[0062] Example 2 Inhibition Test of Bacillus subtilis HMB27703 on Mycelia Growth of R. solani of Cotton

[0063] (1) Test time and place: In October 2013, it was carried out in the Plant Disease Biological Control Laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences.

[0064] (2) Test method:

[0065] (1) Source of R. solanum tested: R. solanum RH-2 strain was collected from a cotton blight disease strain in Qiu County, Handan City, Hebei Province. It was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. The plant pathology department of the Conservation College identified it as Rhizoctonia solani, and the pathogenicity test showed strong pathogenicity.

[0066] (2) plate determination test:

[0067] First activate Solitaire solanum RH-2 on the PDA plate, and after 3 days of cultivation, in the edge area of ​​the colony, use a puncher to Punch to make bacterial flak...

Embodiment 3

[0072] Example 3 Control effect test of bacillus subtilis HMB27703 on cotton blight

[0073] (1) Test treatment:

[0074] (1) Microbial agent: the liquid preparation of HMB27703 prepared in Example 1; diluted 50 times with water.

[0075] (2) Blank control: clear water

[0076](2) Test method: This test was carried out in the plant disease and biocontrol laboratory of the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. First, mix the vermiculite and cotton field soil according to the ratio of 1:1, sterilize under high pressure at 121 ℃ for 1 hour, and then sterilize again under the same conditions the next day. Inoculate the suspension of Rhizoctonia solani mycelium in 500 grams of soil (about 10 7 per milliliter) 10 milliliters, stir evenly, fill in a plastic flower pot with a diameter of 20 cm, and compact it. The cotton variety was Jifeng 106, and the seeds were soaked in the 50-fold water dilution of the HMB27703 liquid preparation pr...

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Abstract

The invention belongs to the field of agricultural biological control and discloses a strain HMB27703 of bacillus subtilis for controlling cotton rhizoctonia. The collection number of the strain is CGMCC No.11458. The invention further discloses a microbial agent for preparation of the bacillus subtilis and an application of the microbial agent for control of the cotton rhizoctonia. The bacillus subtilis HBM27703 has a high pesticide effect on cotton rhizoctonia, and the average control effect is higher than 80.0%; besides, the bacteriocidal spectrum is wide, and bacillus subtilis has a remarkable control effect on botrytis cinerea and cotton fusarium wilt besides cotton rhizoctonia; moreover, bacillus subtilis has high pertinency, the efficacy lasts long, and pesticide resistance is not prone to be caused; the microbial agent is safe to human and the livestock and causes little environmental pollution; the preparation method is simple, low in cost and simple to use.

Description

technical field [0001] The invention belongs to the field of agricultural biological control, and in particular relates to a bacillus subtilis for preventing and treating cotton bacillus, a microbial agent containing the bacillus subtilis, and also relates to their application in preventing and treating cotton blight. Background technique [0002] Cotton blight is a cotton seedling disease caused by Rhizoctonia solani Kühn, which seriously affects the normal growth of cotton. Cotton blight is particularly serious in low temperature and rainy years. [0003] The control of cotton blight is mainly controlled by chemical seed dressing or coating, but the control effect is not good, and because of the environmental pollution, it does not meet the needs of sustainable development of agricultural ecology. At present, no cotton varieties resistant to blight have been found, and the feasibility of disease-resistant breeding is also very small. Therefore, there is still a lack of e...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12Q1/68C12Q1/04C12R1/125
Inventor 李社增鹿秀云马平郭庆港张晓云王莹
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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