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Method for establishing American vicia high-frequency regeneration system

A high-frequency regeneration, wild pea technology, applied in the field of agricultural cultivation biology, can solve the problems of no research and patent publication, and achieve the effect of good technical effect, fast growth and reproduction, and simple operation.

Inactive Publication Date: 2016-01-06
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although there have been reports on the research on the high-frequency regeneration system of plants, there is no research and patent publication on the establishment of the high-frequency regeneration system of American vetch.

Method used

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  • Method for establishing American vicia high-frequency regeneration system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Step 1

[0037] Put the explant material in 75% alcohol to sterilize for 30 seconds, take it out and rinse it with sterile water for 3 times, then immerse it in 0.1% mercuric chloride for sterilization for about 5 minutes, take it out and rinse it with sterile water 5 times; in a sterile environment, dip the explant material on the S1 medium for callus. S1 medium: honey.

[0038] (2) Step 2

[0039] S2 medium: an aqueous solution of 100 mg / L sodium α-naphthalene acetate and 100 mg / L potassium indolebutyrate.

[0040]The callus obtained in step S1 is immersed in the S2 medium towards the root end for 24 hours.

[0041] (3) Step 3

[0042] S3 medium: an aqueous solution of 100 mg / L sodium α-naphthalene acetate, 100 mg / L potassium indolebutyrate and 100 mg / L potassium dihydrogen phosphate.

[0043] The cuttings with good growth quality obtained in step 2 were inserted into 1 cm of wet sand substrate soaked with S3 medium; the culture conditions were 20° C., 2000 l...

Embodiment 2

[0047] (1) Step 1

[0048] Put the explant material in 75% alcohol to sterilize for 30 seconds, take it out and rinse it with sterile water for 3 times, then immerse it in 0.1% mercuric chloride for sterilization for about 5 minutes, take it out and rinse it with sterile water 5 times; in a sterile environment, dip the explant material on the S1 medium for callus. S1 medium: honey.

[0049] (2) Step 2

[0050] S2 medium: an aqueous solution of 200 mg / L sodium α-naphthalene acetate and 200 mg / L potassium indolebutyrate.

[0051] The callus obtained in step S1 is immersed in the S2 medium towards the root end for 24 hours.

[0052] (3) Step 3

[0053] S3 medium: an aqueous solution of 100 mg / L sodium α-naphthalene acetate, 100 mg / L potassium indolebutyrate and 100 mg / L potassium dihydrogen phosphate.

[0054] The cuttings with good growth quality obtained in step 2 were inserted into 1 cm of wet sand substrate soaked with S3 medium; the culture conditions were 20° C., 3000 ...

Embodiment 3

[0058] (1) Step 1

[0059] Put the explant material in 75% alcohol to sterilize for 30 seconds, take it out and rinse it with sterile water for 3 times, then immerse it in 0.1% mercuric chloride for sterilization for about 5 minutes, take it out and rinse it with sterile water 6 times; in a sterile environment, dip the explant material onto the S1 medium for callus. S1 medium: honey.

[0060] (2) Step 2

[0061] S2 medium: an aqueous solution of 400 mg / L sodium α-naphthalene acetate and 400 mg / L potassium indolebutyrate.

[0062] The callus obtained in step S1 is immersed in the S2 medium towards the root end for 24 hours.

[0063] (3) Step 3

[0064] S3 medium: an aqueous solution of 100 mg / L sodium α-naphthalene acetate, 100 mg / L potassium indolebutyrate and 100 mg / L potassium dihydrogen phosphate.

[0065] The cuttings with good growth quality obtained in step 2 were inserted into 1 cm of wet sand substrate soaked with S3 medium; the culture conditions were 20° C., 300...

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Abstract

The present invention belongs to the field of agricultural cultivation biotechnology, in particular to a method for establishing an American vicia high-frequency regeneration system. The method comprises: selecting a young stem of American vicia as an explant; after sterilization, dipping a callus culture medium S1, transferring the explant into a rooting culture medium S2 and carrying out treatment for 20-26 hours to perform rooting cultivation; and then inserting the explant into a wet sand matrix which is dipped and mixed by a seedling and rooting medium S3, putting the explant in a constant temperature condition of 20-25 DEG C for breeding the seedling, and finally tempering and transplanting the obtained seedling. The high-frequency regeneration system has the characteristics of being quick to grow and reproduce, high in repeatability, simple to operate and easy to control conditions and the like, and the rooting rate can reach up to 100%, and the transplanting survival rate also can reach up to 100%. According to the present invention, not only can a large amount of high-quality American vicia be obtained quickly, but also genetic improvement of plants can be performed by using a biotechnology and a gene engineering method. The planting of American vicia further can meet a demand of ecological environment construction, and is an important measure for promoting sustainable development of livestock husbandry.

Description

technical field [0001] The invention belongs to the field of agricultural cultivation biotechnology, in particular to a method for establishing a high-frequency regeneration system of American vetch. Background technique [0002] American vetch (ViciasepiumLinn), Plant Kingdom, Dicotyledonae, Fabaceae, perennial herb, multi-flowered, is a good nectar source plant, and the whole plant is used as medicine. Harvested in summer, dried or used fresh. The root can be eaten raw, but tastes better when cooked. The seeds contain oil. The medicinal leaves, flowers and fruits have the effects of clearing away heat, reducing inflammation and detoxifying. The plants are beautiful and the flowers are gorgeous, and can be used as ornamental flowers. The budding stage appears earlier, when there are few branches, the stems and branches are low, tender and soft, and the crude protein of its nutrients is as high as 26.9%, and the grass yield is also high at this time. After the beginning...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 殷素会戴荣国罗文华姬聪慧任勤王瑞生罗艺杨金龙
Owner CHONGQING ACAD OF ANIMAL SCI
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