Technology for extracting insoluble dietary fiber from bean dregs by enzyme process

A soluble dietary fiber and enzymatic extraction technology, applied in the field of food deep processing, can solve the problems of low purity of insoluble dietary fiber, inability to separate insoluble dietary fiber from soluble dietary fiber, etc., and achieve easy operation, easy operation and control, and reduce Effects of Environmental Pollution

Inactive Publication Date: 2016-01-13
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] When using the existing technology to extract, it is impossible to comprehensively consider the factors that affect the extraction rate such as the pH value in the extraction process, the concentration of the substrate, the degree of pulverizatio

Method used

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  • Technology for extracting insoluble dietary fiber from bean dregs by enzyme process
  • Technology for extracting insoluble dietary fiber from bean dregs by enzyme process
  • Technology for extracting insoluble dietary fiber from bean dregs by enzyme process

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The reagents used in this embodiment are as follows:

[0029]

[0030] Utilize above-mentioned reagent to prepare solution as follows:

[0031] (1) 0.05mol / LMES-TRIS buffer:

[0032] Weigh 4.3021g MES and 2.6928g TRIS, dissolve them in 374mL of water, adjust the pH to 8.17 with 6mol / L sodium hydroxide (preparation temperature is 25°C, calculated by interpolation method), add water to dilute to 440mL.

[0033] (2) Neutral protease solution:

[0034] Use 0.05mol / LMES-TRIS buffer to prepare a 50mg / mL neutral protease solution, ready for immediate use, and the corresponding enzyme activity is 2500U / mL.

[0035] (3) Phosphate buffer solution of PH=6.8:

[0036] Take 250 mL of 0.2 mol / mL potassium dihydrogen phosphate, add 118 mL of 0.2 mol / mL sodium hydroxide solution, and dilute to 1000 mL with water.

[0037] The process of enzymatic extraction of insoluble dietary fiber in bean dregs of the present embodiment comprises the following steps:

[0038] (1) Dry the bea...

Embodiment 2

[0073] A process for enzymatically extracting insoluble dietary fiber from bean dregs, comprising the steps of:

[0074] (1) drying and pulverizing the bean dregs to obtain bean dregs powder;

[0075] (2) Add 5 ml of distilled water and 5 ml of phosphate buffer solution of pH6.5 to every gram of bean dregs powder to obtain bean dregs slurry;

[0076] (3) add neutral protease to bean dregs slurry and carry out enzymolysis for the first time, the add-on of neutral protease is step (1) described bean dregs powder: neutral protease=1g:0.7mg, obtain the first enzymatic hydrolysis bean dregs slurry ;

[0077] (4) Carrying out the first enzymatic deactivation treatment on the first enzymolysis bean dregs pulp to obtain the first deenzyme bean dregs pulp;

[0078] (5) adding heat-resistant α-amylase to the first enzyme-removing bean dregs slurry for the second enzymolysis, the amount of heat-resistant α-amylase is the bean dregs powder described in step (1): heat-resistant α-amylase...

Embodiment 3

[0085] A process for enzymatically extracting insoluble dietary fiber from bean dregs, comprising the steps of:

[0086] (1) drying and pulverizing the bean dregs to obtain bean dregs powder;

[0087] (2) Add 7 ml of distilled water and 7 ml of phosphate buffer solution of pH7.0 to every gram of okara powder to obtain okara powder;

[0088] (3) adding neutral protease to bean dregs slurry for the first enzymolysis, the addition of neutral protease is the bean dregs powder described in step (1): neutral protease=1g:1mg, to obtain the first enzymolysis bean dregs slurry;

[0089] (4) Carrying out the first enzymatic deactivation treatment on the first enzymolysis bean dregs pulp to obtain the first deenzyme bean dregs pulp;

[0090] (5) adding heat-resistant α-amylase to the first enzyme-removing bean dregs slurry for the second enzymolysis, the amount of heat-resistant α-amylase is the bean dregs powder described in step (1): heat-resistant α-amylase =1g:20 μl, obtain the sec...

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Abstract

The present invention discloses a technology for extracting insoluble dietary fiber from bean dregs by an enzyme process. The processing technology comprises the following steps: preparing bean dregs, oven-drying, smashing, sieving, adding water, adding buffer solution, adding proteases, inactivating enzymes, adding alpha-amylase, inactivating enzymes, conducting centrifugal filtration, washing the filter residues, and drying the filter residues to reach a constant weight. The technology comprehensively considers pH values, substrate concentration, grinding degree, the use conditions of enzymes, the amount of enzymes used and other parameters which have effects on the extraction rate. The processing technology enables the reasonable utilization of bean dregs, and reduces environmental pollution caused by discarded beans. The experimental results of ash measurements show that the insoluble dietary fiber extracted by the enzyme process is high in purity, and has only about 2.8% ash content.

Description

technical field [0001] The invention relates to the technical field of food deep processing, in particular to a process for enzymatically extracting insoluble dietary fiber from bean dregs. Background technique [0002] Dietary fiber refers to polysaccharides that cannot be utilized by the human body. The digestive enzymes in the human gastrointestinal tract cannot digest dietary fiber, so it is not easily absorbed by the human body. This type of polysaccharide mainly comes from complex carbohydrates in plant cell walls, and can also be called non-starch polysaccharides, that is, polysaccharides other than α-glucan. It is divided into soluble dietary fiber and insoluble dietary fiber. The nutritional value of dietary fiber has attracted more and more people's attention, and its research has become more and more in-depth. Now dietary fiber is recognized as the first six nutrients after protein, carbohydrates, fat, vitamins, minerals and water. seven types of nutrients. [...

Claims

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Application Information

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IPC IPC(8): A23L33/21
CPCA23V2002/00A23V2250/5116A23V2300/28
Inventor 吴书建张政吕权峰王璐璐卿名义
Owner GUANGXI UNIV
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