A rapid detection method for pyridine pesticides in plant extracts
A plant extract and detection method technology, applied in the field of detection of harmful substances, can solve the problems of long cycle, high cost, complicated process, etc., and achieve the effects of simple operation, low detection limit and low detection cost.
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Embodiment 1
[0075] Weigh 0.5g of Lycium barbarum extract (accurate to 0.01g) into a 50mL glass centrifuge tube, add 10ng of standard substance to mix the standard, add 10mL of ethyl acetate and 0.2mL of ammonia water, vortex and mix, ultrasonically extract for 20min, and centrifuge at 4000r / min 10min. Take 8 mL of the supernatant, concentrate to dryness, add 1 mL of methanol-0.1% formic acid water (1+9) to constant volume, and pass through a 0.22 μm organic microporous membrane to obtain the extracted and concentrated plant extract sample, which is ready to be injected.
[0076] Using TF-LC-MS / MS method to detect pyridine pesticide residues in plant extracts, TF detection parameters: TF purification column: Cyclone TM -P: 0.5×50mm; mobile phase: A is methanol, B is 0.1% formic acid in water; see Table 3 for gradient elution program and flow rate. Liquid phase detection parameters: Hypersil GOLD aQ C18 (column length 50mm, column inner diameter 2.1mm, packing particle size 3μm), or equiva...
example 2
[0081] Weigh 1g of Lycium barbarum extract (accurate to 0.01g) into a 50mL glass centrifuge tube, add 20ng of standard substance to mix the standard, add 30mL of ethyl acetate, 0.3mL of ammonia water, vortex and mix, ultrasonically extract for 25min, and centrifuge at 5000r / min for 6min . Take 10 mL of the supernatant, concentrate to dryness, add 1 mL of methanol-0.1% formic acid water (1+9) to constant volume, and pass through a 0.22 μm organic microporous membrane to obtain the extracted and concentrated plant extract sample, which is ready to be injected.
[0082] Using TF-LC-MS / MS method to detect pyridine pesticide residues in plant extracts, TF detection parameters: TF purification column: Cyclone TM -P: 0.5×50mm; mobile phase: A is methanol, B is 0.1% formic acid water; gradient elution program and flow rate are shown in Table 4. Liquid phase detection parameters: Hypersil GOLD aQ C18 (column length 50mm, column inner diameter 2.1mm, packing particle size 3μm), or equi...
example 3
[0086] Weigh 2g of Lycium barbarum extract (accurate to 0.01g) into a 50mL glass centrifuge tube, add 40ng of standard substance to mix the standard, add 20mL of ethyl acetate, 0.4mL of ammonia water, vortex and mix, ultrasonically extract for 30min, and centrifuge at 6000r / min for 4min . Take 15 mL of the supernatant, concentrate to dryness, add 1 mL of methanol-0.1% formic acid water (1+9) to constant volume, and pass through a 0.22 μm organic microporous membrane to obtain the extracted and concentrated plant extract sample, which is ready to be injected.
[0087] Using TF-LC-MS / MS method to detect pyridine pesticide residues in plant extracts, TF detection parameters: TF purification column: Cyclone TM -P: 0.5×50mm; mobile phase: A is methanol, B is 0.1% formic acid water; gradient elution program and flow rate are shown in Table 5. Liquid phase detection parameters: Hypersil GOLD aQ C18 (column length 50mm, column inner diameter 2.1mm, packing particle size 3μm), or equi...
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