Unlock instant, AI-driven research and patent intelligence for your innovation.

An immunomodulator for the treatment of severe burns

A purposeful, ex vivo technique, applied in the field of immunomodulators for the treatment of severe burns, can solve problems such as ER function homeostasis imbalance

Active Publication Date: 2018-02-02
FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

ER has a very strong homeostasis system, but there are still many factors that can lead to the homeostasis imbalance of ER function, resulting in the accumulation of a large number of errors or unfolded proteins in the lumen of the endoplasmic reticulum, and stimulate the interaction between ER, Golgi apparatus and nucleus. Signal transduction initiates a series of downstream stress responses, namely endoplasmic reticulum stress (ERS)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An immunomodulator for the treatment of severe burns
  • An immunomodulator for the treatment of severe burns
  • An immunomodulator for the treatment of severe burns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Effect of Sal on the Survival Rate of Severely Burned Mouse Model

[0041] 1. Experimental animals and main reagents

[0042] Male BAL B / c mice, 6-8w, 20±2g, were purchased from the Institute of Experimental Animals, Chinese Academy of Medical Sciences (animal certificate number: SCXK Beijing 2009-0007). Adaptive feeding for 1 week, free access to food and water, room temperature 25 ℃, 12 h circadian rhythm.

[0043] Anesthetic drugs: Ketamine Hydrochloride Injection (Shanghai First Biochemical Pharmaceutical Company, China) - Sumianxin II Injection (developed by Military Veterinary Research Institute, Academy of Military Medical Sciences, China) and 0.9% normal saline (Shandong Luxin Pharmaceutical Group, China) is used after mixing according to the volume ratio of 2:2.5:4.5.

[0044] Sal (purchased from Tocris Bioscience, USA): 10 mg / bottle, dissolve Sal in 5 mL DMSO, store at 4°C after fully dissolved, and use up within 2 weeks. Dilute 20 times with 0....

Embodiment 2

[0051] Example 2: Effect of Sal on the Inflammatory State of Severely Burned Mice

[0052] 1. Experimental animals and main reagents

[0053] The mice and Sal used in this example are the same as those in Example 1. TNF-α, IL-12 ELISA kit: Beijing Dakwei Biotechnology Co., Ltd., China; HMGB1 ELISA kit: Shino-Test Company, Japan.

[0054] 2. Experimental steps:

[0055] ① The replication of the severely burned mouse model and the administration of Sal were the same as those described in Example 1. ② Separate the serum after enucleating the mouse's eyes and collecting blood on 1 day and 3 days after the burn, and store them at -30°C. ③ After the samples were collected, the levels of inflammatory factors such as TNF-α, IL-12 and HMGB1 in the serum were detected according to the operation instructions of the ELISA kit.

[0056] The effects of Sal intervention on the levels of inflammatory factors in the serum of severely burned mice were as follows: figure 2 shown.

[0057]...

Embodiment 3

[0059] Example 3: Effect of Sal treatment on DC functional status in spleen of severely burned mice

[0060] 1. Experimental animals and main reagents

[0061] The mice and Sal used in this example are the same as those in Example 1.

[0062] Mouse CD11c Isolation Kit, Mouse CD4 +T isolation kit: Miltenyi Biltec GmbH, Germany; mouse ELISpot (IL-12 / IL-23 p40, TNF-α), Dual-color ELISPOT kit (IL-4 / IL-10, IFN-γ / IL -2): R&D Systems, USA; anti-mouse FITC-CD80, PE-CD86, FITC-MHC-II: Miltenyi Biltec GmbH, Germany; rabbit anti-mouse GRP78 antibody, rabbit anti-mouse CHOP antibody: EPITOMICS , USA; rabbit anti-mouse caspase-12 antibody: Cell-signaling Technology Company, USA.

[0063] 2. Experimental steps:

[0064] ① The replication of the severely burned mouse model and the administration of Sal were the same as those described in Example 1. ② Mice were sacrificed by neck dislocation 1 day after burn injury, and the spleen tissue was collected, minced and ground, filtered throug...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to an immunomodulator used for severe burn and sepsis treatment; the regulator is compound salubrinal (Sal), and its chemical structural formula is 3-PHENYL-N-[2,2,2-TRICHLORO-1 ‑[[(8‑QUINOLINYLAMINO)THIOXOMETHYL]AMINO]ETHYL]‑2‑PROPENAMIDE, CAS No.: 405060‑95‑9. The present invention finds through animal experiments that Sal can reduce the death rate of severely burned mice, alleviate the inflammatory state of the body, promote the maturation and differentiation of dendritic cells (dendritic cells, DC) in the spleen of severely burned mice, and regulate the function of effector T lymphocytes. state, attenuating effector T apoptosis. Said Sal can alleviate the immunosuppressive state after severe burns through the above-mentioned effects, especially cellular immunosuppression, and then improve the prognosis of severely burned mice, and can be used for the treatment of severe burns and their sepsis complications.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to salubrinal and its application, more specifically, to the application of salubrinal in the preparation of medicines for the treatment of severe burns, infections and sepsis, the salubrinal increases the survival rate of severely burned organisms and reduces excessive Inflammatory response, by regulating the functional state of dendritic cells (DC) and T lymphocytes, it can effectively improve the body's immune dysfunction after severe burns and relieve the body's excessive inflammatory response. Background technique [0002] After severe burns, shock often occurs early and severe, and the patient is exposed to burn stress, blood loss, tissue ischemia / hypoxia and other factors that lead to an unstable state of the internal and external environment. In addition to wound infection, the probability of sepsis (sepsis) is extremely high. high. Sepsis can not only aggravate shock, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/4706A61P17/02A61P31/04A61P37/06
Inventor 姚咏明祝筱梅董宁
Owner FIRST HOSPITAL AFFILIATED TO GENERAL HOSPITAL OF PLA