Combined preparation method of triticale feruloyl oligosaccharide and Arab xylo-oligosaccharide

Abstract

The invention provides a method for combined preparation of triticale feruloyl oligosaccharide and Arab xylo-oligosaccharide by using active carbon column chromatography, relates to a special agricultural product processing and integral processing technology and belongs to the field of food processing. According to the method, triticale bran is used as raw materials, and is treated by high-pressure steam; high-temperature alpha-amylase, glucoamylase and neutral protease are respectively added for removing starch and protein; hot distilled water and absolute ethyl alcohol are sequentially used for washing; vacuum drying is performed; triticale bran insoluble dietary fiber is prepared; xylanase is used for performing controllable enzymolysis on the insoluble dietary fiber; obtained enzymolysis liquid is subjected to active carbon column chromatography; distilled water and ethanol with the concentration being 60% are used as eluate for elution; water washing liquid and ethanol washing liquid ingredients are respectively collected; concentration, spray drying and packaging are performed; and two kinds of oligosaccharide products including the feruloyl oligosaccharide and the Arab xylo-oligosaccharide are obtained. The active carbon column chromatography combined preparation process provided by the invention has the advantages that two kinds of high-added-value oligosaccharide products with different compositions can be simultaneously prepared from the one kind of triticale resources; the effective value addition and the integral utilization of the triticale are realized; and important significance is realized on the industrialized development of triticale special grain resources.

Description

technical field [0001] The invention relates to a joint preparation method of triticale feruloyl oligosaccharides and arabinoxylo-oligosaccharides, relates to the deep processing and comprehensive utilization technology of characteristic agricultural products, and belongs to the field of food processing. Background technique [0002] Triticale bran is the main by-product of triticale processing. It is rich in dietary fiber, mainly arabinoxylan, β-glucan and cellulose. Black wheat bran is also rich in phenolic acids, such as ferulic acid, sinapic acid, vanillic acid, etc., the content of ferulic acid is higher than that of common wheat bran. The controllable enzymatic hydrolysis of insoluble dietary fiber from black wheat bran by xylanase can act on the β-1,4 glycosidic bond of the arabinoxylan chain, thereby releasing feruloyl oligosaccharides and arabino-xylo-oligosaccharides . [0003] Both feruloyl oligosaccharides and arabinoxy-oligosaccharides are functional oligosacc...

AI Technical Summary

Problems solved by technology

[0004] At present, the separation and purification of sugar esters of ferulic acid generally adopts non-polar macroporous adsorption resin, which uses van der Waals force to adsorb phenolic substances, and separates sugar esters of ferulic acid and oligosaccharides containing benzene rings; in addition, it can also be used Gel chromatography, using the principle of molecular size exclusion to separate sugar esters of ferulic acid according to molecular weight, or using preparative reverse-phase high performance liquid chromatography to separate and collect sugar esters of ferulic acid with similar molecular weights through polarity differences , but there is no use of the difference in the adsorption capacity of activated carbon for sugar esters of ferulic acid and oligosaccharides and the difference in the ability of different eluents to resolve the sugar esters of ferulic acid and oligosaccharides adsorbed on activated carbon. Related reports on the separation of oligosaccharides