A method for culturing and screening paclitaxel-producing bacteria from Taxus chinensis
A technology of paclitaxel and strains, applied in the biological field, can solve the problems of limited drug sources, low yield, low paclitaxel content, etc., and achieve the effect of practical and simple operation
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[0027] Cut the fresh yew fruit from Huangshan area into 1cm 2 small pieces. Soak the fruit in 75% alcohol for about 5 minutes, and inoculate it on the following plate medium: Potato 20gL -1 , glucose 20gL -1 , agar 20gL -1 and glucose 40gL -1 , peptone 10gL -1 , yeast extract powder 10gL -1 , agar 20gL -1 , and cultured in a 25°C incubator for 8 days. After the colony grows from the incision, observe the hyphae (colonies) growing from the incision of the material in the petri dish, pick the tip of the mycelium at the incision and transfer it to a new plate medium for culture, and after the colony grows, according to the colony According to the difference in shape, color and growth time, the hyphae on the edge were picked for isolation and culture, the shape of the colony and the neatness of the edge of the colony were observed, and corresponding records were made. After repeated purification by mycelium top purification method, they were transferred to PDA slant medium...
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