Method for enriching polyunsaturated fatty acids by virtue of enzyme method

An unsaturated fatty acid and enrichment technology, applied in fermentation and other directions, can solve the problems of continuous production difficulties, low reaction efficiency, long reaction time, etc., and achieve the effect of solving difficulties in industrialization, improving catalytic efficiency, and short separation time

Active Publication Date: 2016-02-10
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high enrichment cost, difficulty in continuous production, low reaction efficiency and long reaction time caused by product inhibition in the process of enzymatic hydrolysis of fish oil to enrich polyunsaturated glycerides, and provide A method for enriching polyunsaturated glycerides by hydrolyzing fish oil with lipase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 6.5g of crude enzyme solution (75U / mL) containing lipase AYS, add 1.5g of sodium sulfate, add 2g of polyethylene glycol 400 and 2g of tuna oil after mixing, put into a stoppered Erlenmeyer flask and mix evenly, prepare Form a three-phase liquid-phase system with a pH of 7; react under stirring at 200 rpm, and control the reaction at 37° C. for 2 hours. Take another 6.5g of crude enzyme solution (75U / mL), add 2g of tuna oil, and dilute to the same volume with water, and react under the same conditions as a control. After the reaction, set the speed of centrifugation at 3000rpm for 3min. The control group is divided into two phases of oil and water, and the experimental group is divided into upper, middle and lower three liquid phases. The upper liquid phase product is a lipid product phase, and the middle liquid phase is an enzyme-rich phase. By-products such as glycerol are distributed to the lower liquid phase. Take the lipid product phases of the experimental gr...

Embodiment 2

[0028] Take 0.9g of crude enzyme solution (80U / mL) containing lipase PL20000L, add 0.3g of ammonium sulfate, mix well, add 0.3g of polyethylene glycol 600 and 0.3g of tuna oil, put it into a stoppered Erlenmeyer flask and mix evenly , prepared into a three-liquid phase system, with a pH of 4.5; placed on a constant temperature shaker with a rotational speed of 200 rpm, and controlled to react at 37° C. for 0.5 h. Take another 0.9g crude enzyme solution (80U / mL), add 0.3g tuna oil, and dilute to the same volume with water, and react under the same conditions as a control. After the reaction, set the speed of centrifugation at 3000rpm for 3min. The control group is divided into two phases of oil and water, and the experimental group is divided into upper, middle and lower three liquid phases. The upper liquid phase product is a lipid product phase, and the middle liquid phase is an enzyme-rich phase. By-products such as glycerin are distributed to prepare the lower liquid phase....

Embodiment 3

[0030]Take 6.5g of crude enzyme solution (100U / mL) containing lipase TL100L, add 1.5g of sodium sulfate, add 2g of polyethylene glycol 400 and 2g of tuna oil after mixing, put into a stoppered Erlenmeyer flask and mix evenly, and prepare into a three-phase liquid-phase system, with a pH of 7; placed on a constant temperature shaker with a rotation speed of 200 rpm, and reacted at 37° C. for 2 h. Another 6.5 g of crude enzyme solution (100 U / mL) was added to 2 g of tuna oil, diluted with water to the same volume, and reacted under the same conditions as a control. After the reaction, set the speed of centrifugation at 3000rpm for 3min. The control group is divided into two phases of oil and water, and the experimental group is divided into upper, middle and lower three liquid phases. The upper liquid phase product is a lipid product phase, and the middle liquid phase is an enzyme-rich phase. By-products such as glycerin are distributed to prepare the lower liquid phase. Take t...

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Abstract

The invention discloses a method for enriching polyunsaturated fatty acids by virtue of an enzyme method. The method comprises the following steps: (1) preparing an enzyme solution that lipase concentration is 5-20000U / mL, and adding soluble salt, a hydrophilic solvent and fish oil to the enzyme solution so as to prepare a three-liquid-phase system; (2) adding the prepared three-liquid-phase system to a stirring kettle and reacting, standing by or centrifuging until layering after reaction, and collecting a supernatant product so as to obtain a lipid product phase; and (3) adding alkaline liquor to the lipid product phase and adding a hydrophobic organic solvent, standing by and reserving a supernatant phase, recovering the hydrophobic organic solvent, reserving and extracting an upper phase which is an omega-3 enriched glyceride product and regulating pH value of a lower phase to be lower than 2 by virtue of acid; and then extracting by virtue of the hydrophobic organic solvent so as to obtain hydrolyzed fatty acid. The method disclosed by the invention has the advantages of low energy consumption, high raw material utilization rate, mild reaction conditions and the like; and the technical problem of an existing enzymatic hydrolysis method which is difficult to achieve industrialization in the production of fatty acid.

Description

technical field [0001] The invention belongs to the field of bioengineering and food technology, and relates to enzyme separation and application technology, in particular to a method for enriching polyunsaturated fatty acids by hydrolyzing fish oil with lipase. Background technique [0002] In recent years, polyunsaturated fatty acids have become the most important variety of functional oils due to their functions of lowering blood lipids, anti-inflammation, and promoting brain development, and have received extensive attention at home and abroad. The market for PUFAs and their associations is estimated to be worth more than $30 billion. Among them, fish oil, in which EPA and DHA are the main components of polyunsaturated polyunsaturated fatty acids, accounts for 78% of the raw material sources of related industries. However, natural fish oil has problems such as low EPA and DHA content and large quality fluctuations, which are difficult to meet the product formulation req...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 李志刚王永华杨博陈华王卫飞陈华勇
Owner SOUTH CHINA UNIV OF TECH
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