Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for producing N-acetylglucosamine

A technology of acetylamino and glucose, applied in the biological field, can solve the problems of high environmental pollution, low reaction efficiency, difficult process control, etc., and achieve the effect of reducing production costs, reducing safety hazards, and simple product separation

Inactive Publication Date: 2016-02-10
NANJING UNIV OF TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The production method of N-acetylglucosamine is mainly through chemical methods, but this method has the disadvantages of huge environmental pollution, low reaction efficiency, poor product quality, and difficult control of the process, and has been gradually replaced by biological enzymatic degradation of chitin.
However, it is difficult to avoid the use of biological enzymes because the components of the fermentation broth are complex, which greatly increases the difficulty and cost of the later separation of the product.
In addition, the transformation process is difficult to control and is prone to bacterial contamination

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for producing N-acetylglucosamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step 1, preparation of chitin crude enzyme solution

[0029] Pick Chitinolytic bacterium meiyuanensis SYBC-H1 in the culture medium (2g / L glucose, 2g / L peptone, 0.7g / LKH 2 PO 4 , 0.3g / LK 2 HPO 4 ·3H 2 O, 0.4g / LMgSO 4 ·7H 2 O, pH7.0) 37°C, 200rpm for 12h to obtain the seed solution, according to the volume fraction of 5% inoculum into a 7.5L fermenter equipped with a liquid enzyme-producing fermentation medium, the liquid volume is 3L, and the enzyme-producing fermentation medium is (5g / L chitin powder, 5g / L Jerusalem artichoke powder, 5g / L peptone, 5g / L beef extract, 0.7g / LKH 2 PO 4 , 0.3g / LK 2 HPO 4 ·3H 2 O, 0.5g / LMgSO 4 ·7H 2 O) The culture temperature is 26°C, the rotation speed is 250rpm, the ventilation rate is 1vvm, the initial pH is 7.0, and the fermentation liquid is collected after 80 hours of fermentation. The fermentation broth was centrifuged at 7000g to separate the bacteria, and the supernatant, namely the crude chitinase enzyme solution, wa...

Embodiment 2

[0037] Step 1, preparation of chitin crude enzyme solution

[0038] preparation Chitinolytic bacterium meiyuanensis SYBC-H1 seed solution, medium (2g / L glucose, 2g / L peptone, 0.7g / LKH 2 PO 4 , 0.3g / LK 2 HPO 4 ·3H 2 O, 0.4g / LMgSO 4 ·7H 2 O, pH7.0), cultured at 37°C and 200rpm for 12 hours, the seeds were inserted into a 7.5L fermenter with a liquid enzyme-producing fermentation medium with a volume fraction of 7% inoculum, and the liquid volume was 3L, and the enzyme-producing medium was ( 5g / L chitin powder, 5g / L Jerusalem artichoke powder, 5g / L yeast extract, 5g / L peptone, 0.7g / LKH 2 PO 4 , 0.3g / LK 2 HPO 4 ·3H 2 O, 0.5g / LMgSO 4 ·7H 2 O) The culture temperature is 26°C, the rotation speed is 300rpm, the ventilation rate is 1vvm, the initial pH is 7.0, and the fermentation broth is collected after 80 hours of fermentation. The fermentation broth was centrifuged at 8000 g to separate the bacteria, and the supernatant, namely the crude chitinase enzyme solution, wa...

Embodiment 3

[0046] Influence of temperature on adsorption effect in step 2.

[0047] The supernatant of the fermented liquid collected in centrifugation embodiment 1, in the process of step 2 adsorption enzyme, by changing temperature, other conditions are consistent, along with the rising of temperature, adsorption capacity increases, but along with raising further, adsorption The effect becomes smaller instead. The final study showed that the optimum temperature was 20°C (see attached table 2).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a process for producing N-acetylglucosamine. According to the process, chitin production strains are selected to be fermented to obtain chitinase crude enzyme, chitin is added into the chitinase crude enzyme for generating affinity adsorption, the chitin absorbed with the enzyme is separated, pure water is added into the obtained liquid, the liquid is stirred and centrifuged, the supernate is taken, finally protein and pigments are removed, and the supernate is concentrated and dried to obtain the product. According to the process, the chitinase is absorbed by ingeniously utilizing the specific affinity interaction of the substrate chitin and the chitinase, so that most impurities in the crude enzyme are removed, and the chitin absorbed with the enzyme is degraded; in the degrading process, the contamination-preventing measure is adopted; the method is low in energy consumption, easy to operate, and little in pollution; the degradation liquid only contains the product, the substrate and extremely small amount of protein, therefore, the product is easy to separate and high in purity, the purpose of producing N-acetylglucosamine with high medical values by taking chitin with a low value as the raw material is realized at low cost, and the process has significant economic benefits and social benefits.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a process for producing N-acetylglucosamine. Background technique [0002] Chitin (C 8 h 13 o 5 N)n, also known as chitin, chitin or chitin, is a polymer composed of N-acetylglucosamine through glycosidic bonds. important ingredient. It is widely distributed in nature, and its content is second only to cellulose. It is the second natural renewable resource in the world. [0003] N-acetylglucosamine is the latest third-generation health functional food additive in chitin health food series. It can be used as a low-calorie sweetener, and can also be used as a food additive for anti-cancer, anti-cancer, lowering blood fat, and lowering blood pressure. It can also be used as a food additive for diabetics. It is mainly used clinically to enhance the function of the human immune system, inhibit the excessive growth of cancer cells or fibroblasts, and can inhibit and treat cancer and m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/26C12N9/42
CPCY02P20/582
Inventor 陈可泉张阿磊王璟应晗笑曹逊欧阳平凯
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com