Jackfruit tissue culture rapid propagation method

A tissue culture and jackfruit technology, applied in the field of plant reproduction, can solve problems such as restricting the development of planting industry, difficulties, and difficulty in obtaining materials, and achieves the effects of solving factory-based seedling raising, realizing large-scale production, and improving the multiplication coefficient.

Inactive Publication Date: 2016-02-17
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, aerial layering of jackfruit is easily restricted by the mother plant and season, the seedling propagation speed is slow, the coefficient is low, and affected by sap flow, it is difficult to root in aerial layering and cutting propagation, and the survival rate is low.
Therefore, large-scale commercial jackfruit seedling production mainly adopts grafting and propagation. Although there have been improvements in budding, branching, cutting, bud stock seedling grafting, and seedling budding in recent years, there are still some good varieties that are difficult to take root and are affected by the mother plant. And season restrictions, low grafting survival ra

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Selection and disinfection of explants: take vigorously growing strips of defoliated jackfruit buds, cut them into stem segments with 2 to 3 buds, soak them in 2v / v% detergent solution for 5min, and rinse them with tap water 15 to 20 minutes, add 2 to 3 drops of 100 mg / L Tween-20 0.1v / v% mercuric acid for 8 to 10 minutes, rinse with sterile water for 3 to 5 times, and finally use sterile filter paper to remove surface moisture to obtain The implant, wherein the sterile water is distilled water sterilized by high temperature and high pressure. Add 2-3 drops of 100mg / L Tween-20 with 0.1v / v% mercury liter for 8-10min disinfection.

[0025] (2) Germination of stem segments to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into WPM medium at a temperature of 22-26°C, light intensity of 1500lux, and light time of 8-10h / d Cultivate for 25 days under certain conditions, and obtain sterile test-tube plantlets after the buds germinate. Among...

Embodiment 2

[0031] (1) Selection and disinfection of explants: take vigorously growing strips of defoliated jackfruit shoots, cut them into stem segments with 2 to 3 buds, soak them in 2v / v% detergent solution for 5min, and rinse them with tap water 15 to 20 minutes, add 2 to 3 drops of 100 mg / L Tween-20 0.1v / v% mercuric acid for 8 to 10 minutes, rinse with sterile water for 3 to 5 times, and finally use sterile filter paper to remove surface moisture to obtain The implant, wherein the sterile water is distilled water sterilized by high temperature and high pressure.

[0032] (2) Germination of stem segments to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into WPM medium at a temperature of 22-26°C, light intensity of 1500lux, and light time of 8-10h / d Cultured under the condition of 25d, after bud germination, sterile test-tube plantlets were obtained, wherein, 6-BA1.0mg / L, sucrose 30mg / L, agar 5mg / L were added to the WPM medium, and the pH of the mediu...

Embodiment 3

[0038] (1) Selection and disinfection of explants: take vigorously growing strips of defoliated jackfruit buds, cut them into stem segments with 2 to 3 buds, soak them in 2v / v% detergent solution for 5min, and rinse them with tap water 15 to 20 minutes, add 2 to 3 drops of 100 mg / L Tween-20 0.1v / v% mercuric acid for 8 to 10 minutes, rinse with sterile water for 3 to 5 times, and finally use sterile filter paper to remove surface moisture to obtain implant. Wherein, sterile water is distilled water sterilized by high temperature and high pressure.

[0039] (2) Germination of stem segments to obtain sterile test-tube plantlets: Inoculate the explants obtained in step (1) into WPM medium at a temperature of 22-26°C, light intensity of 1500lux, and light time of 8-10h / d Cultivate for 25 days under the same conditions. Obtain sterile test-tube plantlets after bud germination, a wherein, add 6-BA1.5mg / L, sucrose 30mg / L, agar 5mg / L in WPM medium, medium pH is 5.8.

[0040] (3) rap...

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PUM

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Abstract

The invention provides a jackfruit tissue culture rapid propagation method. The method includes the following steps that 1, robustly-growing jackfruit tender shoots are selected, and a segment with 2-3 shoots is obtained after cutting and disinfected; 2, a disinfected explant is placed in a WPM minimal medium for germination inducing, and a sterile tube-tube plantlet is obtained; 3, the test-tube plantlet is placed in a WPM propagation culture medium for rapid propagation culture, and multiple shoots are obtained; 4, the multiple shoots are placed in a WPM strong seedling culture medium for strong seedling culture, and a strong plant is obtained; 5, the strong plant is placed in a 1/2 MS rooting medium for rooting culture, and a complete seedling with a root is obtained; 6, the complete seedling with the root is taken, hardened, transplanted to a sand bed for growth for one month, and transplanted to a large field. The multiplication coefficient of the multiple shoots obtained through the culture method is 30-40 times, the rooting rate of tissue cultured seedlings is 95% or above, the survival rate of the seedlings transplanted to the seedbed is 90% or above, and the purpose of industrialized seedling production is effectively achieved.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a jackfruit tissue culture rapid propagation method. Background technique [0002] Jackfruit (Artocarpusheterophylluslam.), also known as wood pineapple, big tree pineapple, honeydew melon, and tripe fruit, is a tropical fruit tree native to India, and is now widely cultivated in Sri Lanka, Myanmar, Indonesia and other regions. It has been introduced since the Sui and Tang Dynasties. Jackfruit has a cultivation history of more than 1,000 years in my country, mainly in Hainan, and it is cultivated in Guangdong, Guangxi, Yunnan, Fujian, Taiwan, southern Sichuan and other places. [0003] Jackfruit is mainly eaten raw, and it is rich in nutrition. The pulp is rich in sugar, protein, vitamin A, vitamin C, potassium, sodium, calcium, zinc and other elements. , can also be made into fruit juice, jam, preserves and other foods. Jackfruit seeds are rich in starch, and it is also a kind of "...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王晓峰李刚缪剑华李翠肖冬韦莹王一诺韦坤华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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