Jackfruit tissue culture rapid propagation method

Abstract

The invention provides a jackfruit tissue culture rapid propagation method. The method includes the following steps that 1, robustly-growing jackfruit tender shoots are selected, and a segment with 2-3 shoots is obtained after cutting and disinfected; 2, a disinfected explant is placed in a WPM minimal medium for germination inducing, and a sterile tube-tube plantlet is obtained; 3, the test-tube plantlet is placed in a WPM propagation culture medium for rapid propagation culture, and multiple shoots are obtained; 4, the multiple shoots are placed in a WPM strong seedling culture medium for strong seedling culture, and a strong plant is obtained; 5, the strong plant is placed in a 1 / 2 MS rooting medium for rooting culture, and a complete seedling with a root is obtained; 6, the complete seedling with the root is taken, hardened, transplanted to a sand bed for growth for one month, and transplanted to a large field. The multiplication coefficient of the multiple shoots obtained through the culture method is 30-40 times, the rooting rate of tissue cultured seedlings is 95% or above, the survival rate of the seedlings transplanted to the seedbed is 90% or above, and the purpose of industrialized seedling production is effectively achieved.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a jackfruit tissue culture rapid propagation method. Background technique [0002] Jackfruit (Artocarpusheterophylluslam.), also known as wood pineapple, big tree pineapple, honeydew melon, and tripe fruit, is a tropical fruit tree native to India, and is now widely cultivated in Sri Lanka, Myanmar, Indonesia and other regions. It has been introduced since the Sui and Tang Dynasties. Jackfruit has a cultivation history of more than 1,000 years in my country, mainly in Hainan, and it is cultivated in Guangdong, Guangxi, Yunnan, Fujian, Taiwan, southern Sichuan and other places. [0003] Jackfruit is mainly eaten raw, and it is rich in nutrition. The pulp is rich in sugar, protein, vitamin A, vitamin C, potassium, sodium, calcium, zinc and other elements. , can also be made into fruit juice, jam, preserves and other foods. Jackfruit seeds are rich in starch, and it is also a kind of "...

AI Technical Summary

Problems solved by technology

However, aerial layering of jackfruit is easily restricted by the mother plant and season, the seedling propagation speed is slow, the coefficient is low, and affected by sap flow, it is difficult to root in aerial layering and cutting propagation, and the survival rate is low.

Therefore, large-scale commercial jackfruit seedling production mainly adopts grafting and propagation. Although there have been improvements in budding, branching, cutting, bud stock seedling grafting, and seedling budding in recent years, there are still some good varieties that are difficult to take root and are affected by the mother plant. And season restrictions, low grafting survival rate, high cost, etc., the quantity and quality of seedlings restrict the development of its planting industry

[0005] AminMN et al., Biondi and Thorpe (1982), Amin and Jaisval (1987) etc. used the stem segments with axillary buds, terminal buds and axillary buds, and elongated tender stems, stem nodes, branch buds and young inflorescences from the stems of mature trees. Axis, successfully obtained test-tube plantlets of jackfruit through tissue culture, but the explants grow better with the main stem, and it is difficult to obtain materials, and it needs to induce callus to proliferate buds, which is easy to mutate and cannot maintain the parent's Lack of good quality