The embodiment of the invention discloses a seed introduction, domestication and cultivation breeding method of wild lomatogonium rotatum and belongs to the technical field of medicinal plant cultivation. The method includes the steps of wild seed collection, seed germination acceleration, soil selection and preparation, sowing, breeding and harvesting. By selecting whole herbal plants of the wild lomatogonium rotatum with slightly hard fruit pods and carrying out specific temperature-changing germination acceleration treatment, the seed germination rate is obviously increased; under combination with a specific cultivation process, the quantity and growth of weeds are effectively controlled, the plant preservation rate reaches 30%, the flowering uniformity reaches 75%, the yield reaches 40 kg / mu, the resource conservation and sustainable use of the scarce and endangered medicinal plant, namely the lomatogonium rotatum, are realized, the bottleneck of a domestication technology is broken through, and market demands are met through industrialized production.