Application of corn ZmWx gene in increase of corn yield and improvement of grain characteristics

A corn and gene technology, applied in the direction of plant genetic improvement, application, genetic engineering, etc., can solve the problem of few reports on the content of amylose in grains

Inactive Publication Date: 2016-02-24
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although GBSSI has such an important effect on amylose synthesis, there are few reports on whether the overexpression of Wx gene affects the grain amylose content.

Method used

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  • Application of corn ZmWx gene in increase of corn yield and improvement of grain characteristics
  • Application of corn ZmWx gene in increase of corn yield and improvement of grain characteristics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Overexpression of GBSSI improves maize grain yield and amylose content Wx gene cloning and recombination

[0032]Take about 0.1 g of corn kernels in the mid-filling stage, grind them into powder with liquid nitrogen, transfer them to a 1.5 mL centrifuge tube filled with 800 μL extract solution (100 mM Tris, pH 9.0; 200 mM NaCl; 1% sodium laurate; 20 mM EDTA; 5 mDTT), vortex Vortex for 1 minute. Centrifuge at 12,000 rpm for 10 minutes at 4°C, then take 600 μL of the supernatant, add 1 / 10 volume of NaAc (2M, pH 4.0), mix well, add 300 μL of chloroform isoamyl alcohol (24:1) and 300 μL of water-saturated phenol, Vortex for 2 minutes. Then centrifuge at 12000 rpm at 4°C for 10 minutes, take 500 μL of supernatant, add 500 μL of chloroform-isoamyl alcohol (24:1), and vortex extract for 2 minutes. Then centrifuge at 12,000 rpm for 10 minutes at 4°C, take 360 ​​μL of supernatant, add 120 μL of 8MLiCl, precipitate at 4°C for 3 hours, and centrifuge at 12,000 rpm for...

Embodiment 2

[0053] Example 2: Polymerization of transgenic ZmWx and mutant AGPase genes improves corn grain yield and starch content

[0054] The ability of GBSSI to control amylose synthesis is not only affected by the protein content of GBSSI, but also restricted by factors such as the substrate ADPG. In the case of sufficient supply of the substrate ADPG, the content of amylose synthesized by GBSSI may be further increased. In the process of starch synthesis, AGPase catalyzes G1P and ATP to generate ADPG and inorganic phosphate PPi, which provide precursor substance ADPG for starch synthesis. It is generally believed that the synthesis of ADPG is an important regulatory point of starch synthesis, and AGPase is an important rate-limiting enzyme in the starch synthesis pathway. Therefore, the obtained mutant AGPase-overexpressed transgenic maize was cross-polymerized with the GBSSI-overexpressed transgenic maize, and the grain traits, starch content and composition were detected.

[0055...

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Abstract

The invention discloses an application of a corn ZmWx gene in increase of the corn yield and improvement of grain characteristics. A ZmWx gene sequence is cloned in corn endosperm cDNA (complementary deoxyribonucleic acid), the ZmWx gene encodes GBSSI (granule-bound starch synthase I), the GBSSI and an endosperm specific promoter form a fusion gene, the fusion gene is recombined in a plant expression carrier with a transgenic technology to transform corn, and corn transgenetic plants with yield increased and grain characteristics improved are obtained; alternatively, GBSSI overexpressed corn and mutant AGPase overexpressed high-starch corn are hybridized, a transgenetic polymer is obtained through screening, and the corn transgenetic plants whose grain yield, starch content and hundred-grain weight are remarkably higher than those of recipient materials are produced. The application provides a basis and a means for development and preparation of high-starch and high-amylose starch corn and also develops a new approach for increase of the corn yield and improvement of the starch quality.

Description

technical field [0001] The invention belongs to the field of crop bioengineering breeding, and specifically relates to the application of a corn ZmWx gene in increasing corn yield and improving grain traits. Background technique [0002] Starch is an indispensable component of human food, an important raw material for food industry and chemical industry, and plays an important role in human life. With the rapid development of the starch processing industry, the use of starch has been continuously expanded, especially the production of fuel ethanol, resulting in a sharp increase in the demand for starch. As the largest crop in the world, corn has a higher yield than wheat and rice. Among various crop starches, corn starch has the best chemical composition and the highest yield. In 2013, the global starch production was about 68.8 million tons, of which corn starch was about 61 million tons, accounting for 89% of the total. More than 90% of my country's starch processing ind...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/82A01H5/00
CPCC12N9/1051C12N15/8205C12N15/8245C12N15/8261C12Y204/01021
Inventor 张举仁赵丫杰王慧李朝霞
Owner SHANDONG UNIV
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