On-site fermented bacillus licheniformis preparation for aquaculture farm and applications thereof

Abstract

The invention relates to an on-site fermented bacillus licheniformis preparation for aquaculture farm and applications thereof. The preparation comprises the following raw materials: bacillus licheniformis powder, colistin sulfate, natamycin, inosine, corn flour, corn steep liquor powder, sodium chloride, serine, fructose, and brown sugar. The colistin sulfate and natamycin in the preparation can inhibit the growth of fungi (such as pathogenic fungi, yeast, etc.) and gram negative bacteria (such as colibacillus, salmonella, etc.) during the fermentation process. The spore germinator (inosine, fructose, and serine) can rapidly promote the germination of spores of bacillus licheniformis and improve the germination rate of spores. The provided preparation can be applied to the onsite fermentation and propagation in a simple and crude aquaculture farm. The required equipment is simple. The using amount of bacterium is little. The cost is low. The fermentation broth obtained through onsite fermentation has the advantages of high content and good activity. The preparation can improve the water quality and intestinal functions, prevent disease, promote growth, increase output, and improve product quality.

Description

technical field [0001] The invention relates to the field of aquatic micro-ecological bacterial agents, in particular to a bacillus licheniformis preparation and application method applied to on-site fermentation in aquaculture farms. Background technique [0002] With the development of large-scale aquaculture, intensification and intensive farming technology, the residual bait, excrement and other organic pollutants in the pond are also increasing day by day. The decomposition of organic pollutants requires a large amount of dissolved oxygen and produces a large amount of toxic and harmful substances. , such as ammonia nitrogen, nitrite, sulfide, etc. With the increase of these toxic and harmful substances, it will not only affect the growth and reproduction of aquatic animals, but even cause poisoning death in severe cases. The number of pathogenic microorganisms in the water body is directly related to the concentration of ammonia nitrogen, nitrite, and sulfide in the w...

AI Technical Summary

Problems solved by technology

[0006] The second is that the early stage of spore germination takes a long time, usually 4 hours before the spores will start to germinate. At this time, due to the large-scale reproduction of yeast and lactic acid bacteria in the "activation" solution, the pH drops rapidly, which can already inhibit the germination and reproduction of spores. , thereby greatly affecting the germination of spores;

[0007] The third is that the high-nutrient medium is also susceptible to harmful bacteria such as Escherichia coli and fungi, resulting in poor effects after application and still causing side effects;

[0008] Fourth, the amount of spores used is large, and the effect is unstable

[0009] However, there has not been disclosed so far a Bacillus licheniformis preparation that can better control miscellaneous bacteria and promote the rapid germination of lichen spores, which is applied to the on-site fermentation and propagation of aquaculture farms.

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