Bacillus natto preparation and application for on-site fermentation in livestock and poultry farms

Abstract

The present invention relates to a bacillus natto preparation used for livestock and poultry culturing farm on-site fermentation and a preparation method and application thereof, the bacillus natto preparation is prepared from the raw materials of bacillus natto powder, colistin sulfate, natamycin, L-alanine, corn flour, soybean meal powder, potassium chloride, threonine and brown sugar; the colistin sulfate and the natamycin in the bacillus natto preparation can inhibit growth of pathogenic fungi, yeast and other fungi and escherichia coli, salmonella and other gram-negative bacteria in the fermentation process, the L-alanine and threonine and other spore germination agents are used to fast promote germination of bacillus natto spores and increase spore germination rate; fermentation propagation operation can be realized in a simple and crude livestock and poultry farm, facility demand is simple, bacteria use amount is less, cost in low, on-site fermentation liquor bacteria is high in content and good in activity, and the bacillus natto preparation can regulate livestock and poultry intestines, prevent diseases and promote growth, improves farming environment, increase production and improve quality.

Description

technical field [0001] The invention relates to the field of microbial feed additives, in particular to a bacillus natto preparation and application applied to on-site fermentation in livestock and poultry farms. Background technique [0002] It is reported that Bacillus natto can produce many antibiotics, such as bacitracin, polymyxin and 2,6-pyridine dicarboxylic acid, which are effective against Shigella, primary Escherichia coli O-157, O-111, O- 144, typhoid bacteria, salmonella, etc., all have a strong inhibitory effect, so Bacillus natto has a strong antibacterial activity. Dr. Xumi Yangxing conducted research on the antibacterial function of natto. After they added natto to O-157 E. coli for 4 days, all E. coli were inhibited and died. Kyosuke Ozawa (1983) found that the spores of Bacillus natto have antibacterial effects on pathogenic Escherichia coli (such as O-111, O-144) and Salmonella, and can also kill enterohemorrhagic Escherichia coli O-157 . Studies by Osa...

AI Technical Summary

Problems solved by technology

[0006] The second is that the early stage of spore germination takes a long time, usually 4 hours before the spores will start to germinate. At this time, due to the large-scale reproduction of yeast and lactic acid bacteria in the "activation" solution, the pH drops rapidly, which can already inhibit the germination and reproduction of spores. , thereby greatly affecting the germination of spores;

[0007] The third is that the high-nutrient medium is also susceptible to infection of Escherichia coli, the original plant pathogenic bacteria and other harmful bacteria, resulting in poor effects after application and still have adverse effects;

[0008] Fourth, the amount of spores used is large, and the effect is unstable

[0009] However, there has not been disclosed so far about a Bacillus natto preparation that can better control miscellaneous bacteria and promote the rapid germination of natto spores, which is applied to the on-site fermentation and propagation of livestock and poultry farms.

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