Preparation method of sargassum oligosaccharide and application of sargassum oligosaccharide in hypoglycemic drugs

A technology of sargassum and oligosaccharides, applied in the direction of drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., to achieve the effects of good inhibition of activity, promotion of glucose consumption, and reduction of enzyme dosage

Active Publication Date: 2016-04-13
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the hypoglycemic activity of the sargassum oligosaccharides obtained by ultrasonic-assisted step-by-step enzymatic hydrolysis in the present invention has no other research reports or patents.

Method used

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  • Preparation method of sargassum oligosaccharide and application of sargassum oligosaccharide in hypoglycemic drugs
  • Preparation method of sargassum oligosaccharide and application of sargassum oligosaccharide in hypoglycemic drugs
  • Preparation method of sargassum oligosaccharide and application of sargassum oligosaccharide in hypoglycemic drugs

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Dried and pulverized Sargassum, added 95% ethanol to the dry powder of Sargassum and refluxed for 2 hours, the ratio of the weight of the dry powder to the volume of ethanol was 1:20, removed the ethanol after the reflux, and dried to obtain the dried and degreased Sargassum powder Distilled water is added to the Sargassum powder, wherein the mass of Sargassum and the volume ratio of distilled water are 1:30, and the water extraction method is used to extract for 2 hours at 90 ° C. After the extraction is completed, filter, and the filter residue is extracted twice by repeating the above steps. The obtained filtrate was mixed, concentrated, added 3 times the volume of 95% ethanol, left to settle at 4°C for 12 hours, centrifuged to collect the precipitate, vacuum freeze-dried and pulverized the crude polysaccharide of Sargassum;

[0026] (2) Adding distilled water to the crude polysaccharide of Sargassum obtained in step (1), wherein the mass ratio of the crude polysa...

Embodiment 2

[0068] (1) Dried and pulverized Sargassum, added 95% ethanol to the dry powder of Sargassum and refluxed for extraction for 1 hour. The ratio of dry powder weight to ethanol volume was 1:15. After the reflux was completed, the ethanol was removed and dried to obtain dry degreased Sargassum powder Distilled water is added to the Sargassum powder, wherein the mass of Sargassum and the volume ratio of distilled water are 1:25, and the water extraction method is used to extract for 2 hours at 85 ° C. After the extraction is completed, filter, and the filter residue is extracted twice by repeating the above steps. The obtained filtrate was mixed, concentrated, added 3 times the volume of 95% ethanol, left to settle at 4°C for 8 hours, centrifuged to collect the precipitate, vacuum freeze-dried and pulverized the Sargassum crude polysaccharide;

[0069] (2) Add distilled water to the Sargassum crude polysaccharide obtained in step (1), wherein the mass ratio of the Sargassum crude po...

Embodiment 3

[0079] (1) Dried and crushed Sargassum, added 95% ethanol to the dry powder of Sargassum and refluxed for 2 hours, the ratio of the weight of the dry powder to the volume of ethanol was 1:17, removed the ethanol after the reflux, and dried to obtain dry and defatted Sargassum powder Distilled water is added to the Sargassum powder, wherein the mass of Sargassum and the volume ratio of distilled water are 1:27, and the water extraction method is used to extract at 90° C. for 2 hours. After the extraction is completed, filter, and the filter residue is extracted 3 times by repeating the above steps. The obtained filtrate was mixed and then concentrated, and 4 times the volume of 95% ethanol was added. After standing at 7°C for 10 hours, the precipitate was collected by centrifugation, and the crude polysaccharide of Sargassum sargassum was pulverized after vacuum freeze-drying;

[0080] (2) Adding distilled water to the Sargassum crude polysaccharide obtained in step (1), wherein...

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Abstract

The invention discloses a preparation method of sargassum oligosaccharide. The preparation method comprises the steps that fine polysaccharide obtained through degreasing and deproteinizing of sargassum is taken as a raw material, ultrasonic treatment is utilized, alginate lyase, mannase, xylanase and pectinase are sequentially added to perform enzymolysis on the polysaccharide, the polysaccharide which is not degraded fully is removed through an ethyl alcohol sedimentation method, supernate is centrifuged and screened by a molecular sieve to obtain retained matter, freeze drying is performed, and the sargassum oligosaccharide is prepared. The prepared sargassum oligosaccharide has the high inhibitory activity on alpha-glucosidase, the IC50 value is 4.82 mg/mL, the dose dependency is presented, and meanwhile the obvious promoting effect on glucose consumption of insulin-resistant HepG2 cells is achieved. According to the preparation method, the non-specific commercial enzymes are adopted, the process route is simple and reasonable, the method is suitable for industrialization, the preparation amount of the hypoglycemic active oligosaccharide is increased, and meanwhile the enzyme consumption and production cost are reduced; the method is an effective method for preparing the sargassum oligosaccharide and can be applied to hypoglycemic drugs, health care products and food.

Description

technical field [0001] The invention relates to a preparation method of sargassum oligosaccharide and its application in hypoglycemic drugs, belonging to the production process of functional oligosaccharide enzymatic hydrolysis. Regarding a method for preparing sargassum oligosaccharides by step-by-step enzymatic hydrolysis and its application in the application of hypoglycemic drugs, health products, and food, the present invention is characterized in that ultrasonic-assisted step-by-step enzymolysis of sargassum polysaccharides to prepare oligosaccharides. Background technique [0002] Diabetes is a metabolic disease characterized by high blood sugar. At present, hypoglycemic drugs are either insufficient in efficacy or have significant side effects, and the development of health foods with significant hypoglycemic effects and no side effects has become a current global research hotspot. Seaweed polysaccharides and their degradation products—specific seaweed oligosacchari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04A61K31/702A61P3/10
CPCA61K31/702C12P19/04C12P19/14
Inventor 赵超刘斌杨成凤肖正
Owner FUJIAN AGRI & FORESTRY UNIV
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