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A photocrosslinked biomimetic hydrogel and its preparation and application

A hydrogel and photocrosslinking technology, applied in prostheses, bandages, surgery, etc., can solve the problems of non-specific protein adsorption, poor antibacterial properties, etc., achieve rapid molding conditions, improve hydrophilicity, and broad-spectrum antibacterial effect of effect

Active Publication Date: 2018-10-09
广州佰斯伦医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still shortcomings such as poor antibacterial properties under physiological conditions and easy to cause non-specific protein adsorption, which limit its clinical application.

Method used

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  • A photocrosslinked biomimetic hydrogel and its preparation and application
  • A photocrosslinked biomimetic hydrogel and its preparation and application
  • A photocrosslinked biomimetic hydrogel and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A preparation method of glycidyl methacrylate-phosphoric acid dicholine chitosan hydrochloride hydrogel, comprising the steps of:

[0043] Step (1): Synthesis of Phosphodicholine Chitosan Hydrochloride (PDCCs)

[0044] Get 200mg of 6-O-triphenyl methyl etherified chitosan (Cs-Tr) obtained by modification of chitosan (x / n=0) and dissolve it in 10mL of anhydrous N, N-dimethylacetamide, Add 1.05mL of triethylamine and 0.475mL of carbon tetrachloride at the same time; slowly add 10mL of isopropanol to dissolve 0.475mL of disubstituted choline phosphonate The molar ratio is 1:5, stirred at room temperature for 12 hours; spin-dried the solvent, added formic acid, and stirred at room temperature for 2 hours; spin-dried formic acid, dialyzed with normal saline and deionized water, freeze-dried to obtain phosphoric acid dicholine chitosan Sugar hydrochloride in which the degree of substitution of phosphoricholine groups is 42%.

[0045] Step (2): Synthesis of glycidyl methacry...

Embodiment 2

[0050] A preparation method of glycidyl methacrylate-phosphoric acid dicholine chitosan hydrochloride hydrogel, comprising the steps of:

[0051] Step (1): Synthesis of Phosphodicholine Chitosan Hydrochloride (PDCCs)

[0052] Get 200mg of 6-O-triphenyl methyl etherified chitosan (Cs-Tr) obtained by modification of chitosan (x / n=0) and dissolve it in 10mL of anhydrous N, N-dimethylacetamide, Add 1.05mL of triethylamine and 0.475mL of carbon tetrachloride at the same time; slowly add 10mL of isopropanol to dissolve 0.285mL of disubstituted choline phosphonate The molar ratio of the solution was 1:3, and the reaction was stirred at room temperature for 12 hours; the solvent was spin-dried, and formic acid was added, and stirred at room temperature for 4 hours; Sugar hydrochloride with a degree of substitution of 25% phosphodicholine groups.

[0053] Step (2): Synthesis of glycidyl methacrylate-phosphodicholine chitosan hydrochloride (PDCCs-GMA)

[0054] Dissolve 0.5 g of phosp...

Embodiment 3

[0057] Example 3: Evaluation of antibacterial properties of glycidyl methacrylate-phosphodicholined chitosan hydrochloride hydrogel.

[0058] With Escherichia coli (Escherichia coli) (commercially available) (Gram-negative bacterium) as bacterial model, measure the glycidyl methacrylate-phosphodicholine chitosan hydrochloride hydrogel prepared in Example 1 at 37 DEG C , Bacteriostasis under the condition of pH=7. Escherichia coli bacterial liquid OD598=0.1 (10 6 ~10 8 CFU / mL) 100 μL was added dropwise on the surface of glycidyl methacrylate-phosphate dicholine chitosan hydrogel hydrogel (1cm 2 ), cultured in a constant temperature and humidity shaker at 37°C with a humidity greater than 90% for 3 hours, then put it into sterile water and sonicate it for 10 minutes to elute surface bacteria. Take 50 μL of the eluate and evenly spread it on LB solid medium for 18 hours to observe the growth of bacteria. The results show that the antibacterial rate of glycidyl methacrylate-pho...

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Abstract

The invention discloses a photo-crosslinking bionic hydrogel and preparation and application thereof. The monomer of the photo-crosslinking bionic hydrogel is glycidyl methacrylate-citicoline alkalization chitosan hydrochloride. The preparation method comprises the three steps of synthesis of citicoline alkalization chitosan hydrochloride, synthesis of glycidyl methacrylate-citicoline alkalization chitosan hydrochloride and addition of a photoinitiator to obtain the glycidyl methacrylate-citicoline alkalization chitosan hydrochloride hydrogel. The photo-crosslinking bionic hydrogel molding conditions are rapid and mild, a citicoline basic group of a local area branch dual-positive-charge structure can improve hydrophilia, cytotoxicity, antibacterial property and protein adsorption restraining, the obtained bionic hydrogel has the remarkable broad-spectrum antibacterial effect on the physiological environment condition, is non-toxic for normal cells, can restrain protein adsorption, and has the great application value in the biological medical fields of medical treatment, healthcare and the like.

Description

technical field [0001] The invention belongs to the technical field of polymer gel, and in particular relates to a photocrosslinked biomimetic hydrogel and its preparation and application. Background technique [0002] Hydrogel is a polymer material with a three-dimensional network structure formed by cross-linking through covalent bonds, hydrogen bonds or van der Waals forces. Because its physical properties are similar to living tissues, it often has excellent biocompatibility, so it shows broad application prospects in biomedical fields such as tissue engineering, drug delivery systems, implants and interventional medical devices. [0003] The commonly used crosslinking methods for preparing gels include physical crosslinking, chemical crosslinking, high-energy ray irradiation crosslinking and photocrosslinking. The mechanical properties and stability of physical cross-linked gels are not ideal; the chemical initiator of chemical cross-linking increases its cytotoxicity ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F299/00C08F2/48C08F251/00C08F220/32C08B37/08A61L15/28A61L15/44A61L27/20A61L27/54A61L27/52A61L31/04A61L31/14A61L31/16
CPCA61L15/28A61L15/46A61L27/20A61L27/52A61L27/54A61L31/042A61L31/145A61L31/16A61L2300/232A61L2300/404C08B37/003C08F2/48C08F251/00C08F299/024C08L5/08C08F220/325
Inventor 曾戎赵云斐马柳波屠美赵剑豪
Owner 广州佰斯伦医疗器械有限公司
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