A kind of method to improve the ionization efficiency of oligosaccharide

An ionization and oligosaccharide technology, applied in the field of biochemical analysis, can solve the problems that have not yet been seen, and achieve the effects of improving ionization efficiency, uniform crystallization, and beneficial to sugar chain structure analysis.
CN105527334BInactive Publication Date: 2018-07-24FUDAN UNIV
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FUDAN UNIV
Publication Date
2018-07-24
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the field of biochemical analysis, and relates to a method for improving the ionization efficiency of oligosaccharides, especially a method for improving the ionization of oligosaccharides in matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS); the method adopts 6 ‑Hydrazine nicotinamide (HYNIC) is used as a matrix to analyze oligosaccharides, which selectively improves the ionization efficiency of oligosaccharides and inhibits the ionization of other analytes such as peptides. The 6-hydrazine nicotinamide in the present invention has the structure of formula (I); when the 6-hydrazine nicotinamide is used as a matrix to analyze oligosaccharides, it has high sensitivity, uniform crystallization, strong salt tolerance, and tandem mass spectrum fragmentation The advantages such as abundant information; The method of the present invention, adopts described 6 hydrazine nicotinamide as matrix analysis oligosaccharide, can selectively improve the ionization efficiency of oligosaccharide, and suppressed other analyte such as peptide segment Ionization has the advantages of simple operation, simple steps, economical and time-saving, and no desalination is required.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The invention belongs to the field of biochemical analysis, and relates to a method for ionization of oligosaccharides, in particular to a method for improving ionization efficiency of oligosaccharides, in particular to a method for improving oligosaccharides in matrix assisted laser desorption time-of-flight mass spectrometry (MALDI-TOFMS) Medium ionization method: This method uses 6-hydrazine hydrazine amide (HYNIC) as a matrix to analyze oligosaccharides, which selectively improves the ionization efficiency of oligosaccharides and inhibits the ionization of other analytes such as peptides. Background technique

[0002] Oligosaccharides are an important type of information substance in the body and have many biological functions. They are generally composed of 3-10 monosaccharide molecules connected by glycosidic bonds. The oligosaccharide has important physiological functions, such as activating the cell's self-defense system, determining cell recognitio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More