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Brevundimonas naejangsanensis S3 and application thereof

A technology of brevundimonas and vesicles, applied in the field of microorganisms, to achieve the effects of easy management, less restricted conditions, and less environmental interference factors

Active Publication Date: 2016-05-11
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there is a lack of antibiotic-degrading bacteria that can tolerate various heavy metal environments in the prior art.

Method used

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  • Brevundimonas naejangsanensis S3 and application thereof
  • Brevundimonas naejangsanensis S3 and application thereof
  • Brevundimonas naejangsanensis S3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Pseudomonas vesicularis (Brevundimonasnaejangsanensis) S3; deposited in the General Microbiology Center of the China Microbial Culture Collection and Management Committee, the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, with the preservation number of CGMCCNO.11072, deposited on July 10, 2015.

[0025] Brevundimonasnaejangsanensis S3 can grow in the pH range of 6-8; the suitable growth temperature is 25℃~35℃.

[0026] Brevundimonasnaejangsanensis S3 was cultured in 50 mL of beef extract peptone liquid medium, and gentamicin (G), neomycin (N), streptomycin (S), and ampicillin ( A), the concentration of penicillin sodium (P), cephalosporin (C), sulfathiazole (STZ) is 32mg / L, 32mg / L, 128mg / L, 64mg / L, 64mg / L, 64mg / L, 64mg / L L, Cu 2+ , Zn 2+ The concentrations were 200 mg / L and 400 mg / L, respectively, and the degradation rate of antibiotics reached more than 90% after culturin...

Embodiment 2

[0028] In this embodiment, Brevundimonasnaejangsanensis (Brevundimonasnaejangsanensis) S3 is obtained by the following steps.

[0029] Soil samples were collected in the mulberry garden of Jiangsu University of Science and Technology. Sampling was carried out according to the 5-point sampling method, and then all the collected soils were mixed, air-dried, and passed through a 100-mesh sieve to obtain soil samples. It was then treated with 300 mg / kg copper sulfate and 1000 mg / kg zinc sulfate after 84 days. Weigh 1 g of soil sample and add it to 99 ml of sterile saline, shake at 120 r / min at 25°C for 2 h to make soil 10 -4 Diluent for use. Take the dilution solution and spread it on the plate, incubate at 30°C for 2-4 days. The plate medium is basal mineral salts (MSM) medium (g / L): K 2 HPO 4 5.8, KH 2 PO 4 4.5, (NH 4 ) 2 SO 4 2.0, MgCl 2 0.16, CaCl 2 0.02, Na 2 MoO 4 ·2H 2 O0.0024, FeCl 3 0.0018, MnCl 2 ·2H 2 O0.0015, 18 g of agar, pH=7.0, sterilized by moist ...

Embodiment 3

[0031] Brevundimonasnaejangsanensis S3, an antibiotic-degrading bacterium resistant to heavy metals in this embodiment, can be used to degrade antibiotics in heavy metal-polluted environments.

[0032] Brevundimonasnaejangsanensis S3 was added to the environment polluted by heavy metals and antibiotics, and the environment temperature was 25°C-35°C, and the environment pH value was 6-8. Brevundimonasnaejangsanensis S3 in this embodiment can be used for remediation of contaminated soil or water.

[0033] Select gentamicin, neomycin, streptomycin, ampicillin, penicillin sodium, cephalosporin and sulfathiazole so that the final concentration reaches 32mg / L, 32mg / L, 128mg / L, 64mg / L, 64mg / L, respectively. L, 64mg / L and 64mg / L, Cu 2+ , Zn 2+ Concentrations of 200mg / L, 400mg / L compound contaminated soil 100g, the vesicular Brevundimonasnaejangsanensis (Brevundimonasnaejangsanensis) S3 cultivated in the beef extract peptone liquid medium in the early stage of stationary phase was sp...

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Abstract

The invention relates to Brevundimonas naejangsanensis S3 and application thereof. The Brevundimonas naejangsanensis S3 is preserved in China General Microbiological Culture Collection Center on July 10th, 2015, and the preservation number of the Brevundimonas naejangsanensis S3 is CGMCC No.11072. The Brevundimonas naejangsanensis S3 has the advantages that the Brevundimonas naejangsanensis S3 can degrade antibiotics, avoid competition among mixed microorganisms and avoid antagonism among different microorganism metabolism products; meanwhile, due to the fact that single microorganism material is used, degrading conditions can be controlled easily, and management is facilitated; the Brevundimonas naejangsanensis S3 is added as dominant bacterial community during environment remediation and is few in environment interference factors, few in limitation conditions, and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a heavy metal-resistant antibiotic-degradable microorganism and its application in environmental restoration. Background technique [0002] The addition of veterinary antibiotics and heavy metal elements such as copper and zinc to feed can not only prevent and treat various diseases, but also improve feed efficiency and promote the growth of livestock and poultry. Therefore, veterinary antibiotics and trace metal elements are widely used as feed additives in intensive Livestock and poultry farms. However, antibiotics and trace metal elements cannot be completely metabolized and absorbed in animals. Except for a small part remaining in the body, most of them are excreted in the form of original drugs and metabolites with animal feces and urine, and enter the ecological environment. Because livestock and poultry manure organic fertilizer can provide rich nutrien...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 杨统一杨芬
Owner JIANGSU UNIV OF SCI & TECH
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