Pharmaceutical composition and health functional food containing red ginseng concentrate having enhanced compound K for preventing and treating non-alcoholic fatty liver disease

A functional food, non-alcoholic technology, applied in the direction of drug combination, food ingredients containing natural extracts, functions of food ingredients, etc., can solve the problems of patients developing liver cirrhosis, non-alcoholic fatty liver inflammation, etc.

Inactive Publication Date: 2016-05-18
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nonalcoholic fatty liver disease can be simple lipidosis, but there is a problem that nonalcoholic fatty liver inflammation occurs in about 10% to 20% of patients with nonalcoholic fatty liver disease, and about 9% to 25% of patients develop cirrhosis
Therefore, in order to prevent non-alcoholic fatty liver disease from developing into cirrhosis, fat accumulation in the liver should be prevented, but the situation is that there is no other method than reducing calorie intake or increasing calorie expenditure

Method used

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  • Pharmaceutical composition and health functional food containing red ginseng concentrate having enhanced compound K for preventing and treating non-alcoholic fatty liver disease
  • Pharmaceutical composition and health functional food containing red ginseng concentrate having enhanced compound K for preventing and treating non-alcoholic fatty liver disease
  • Pharmaceutical composition and health functional food containing red ginseng concentrate having enhanced compound K for preventing and treating non-alcoholic fatty liver disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Preparation of Compound K Fortified Red Ginseng Concentrate

[0061] The ginseng used in this study was dried red ginseng (4-year-old root) prepared by adding 10 times (w / v) water or ethanol as the raw material and performing three 15-hour reflux extractions at 70°C to 80°C at 200 rpm Red ginseng extract was extracted, and then a concentrate having 70 brix or more was prepared by cooling the red ginseng extract twice in a water bath at room temperature or low temperature, filtering, and concentrating under reduced pressure .

[0062] Dilute the concentrate with 10-fold (w / v) water, adjust to pH 4.3 by adding citric acid, then add 2% (v / v) enzyme cytolasePCL5 (DSMCompany, France) and react at 56°C, 200rpm for 24 hours to 85 hours.

[0063] Then, compound K-enhanced red ginseng concentrate was prepared by heating the concentrate in a boiling water bath for 30 minutes until no more reaction occurred to inactivate the enzyme, and then by concentrating it unde...

experiment example 1

[0064] Experimental Example 1: Validation of curative effect through animal model of fatty liver in vivo

[0065] In order to study the effect of compound K fortified red ginseng concentrate on improving the function of non-alcoholic fatty liver, 5-week-old C57BL / 6 male mice were used to establish a high-fat diet-induced obesity animal model and conduct in vivo experiments.

[0066] This model is mainly used in rodents, and the general situation is to verify the effect by inducing obesity in the model with a high-fat diet, and then administering the sample to be tested to the model. Therefore, we attempted to examine the efficacy of the compound K-fortified red ginseng concentrate prepared by the above-mentioned Example 1 through animal experiments.

[0067] As shown in Table 1 below, the experimental animals were divided into six (6) groups in total and administered with defined diets and samples for 11 weeks. Animals were orally administered normal diet (ND) or high-fat d...

experiment Embodiment 2

[0100] Experimental Example 2: Analysis of Gene Expression of Proteins Related to Lipid Metabolism in Liver Tissue

[0101] As the first step to study the regulatory mechanism of compound K-enhanced red ginseng concentrate regulating lipid metabolism in liver tissue, the gene expression of major proteins involved in lipogenesis and lipolysis in liver tissue was analyzed by real-time RT-PCR method, The results are shown in Figure 5 middle.

[0102] SREBP-1c (Sterol Regulatory Element Binding transcription factor 1), FAS (fatty acid synthase), ACC (acetyl-CoA carboxylase), LXRα (liver X receptor body α).

[0103] PCR for the housekeeping gene 36B4 (acid ribosomal phosphoprotein PO) was used as an internal standard for analysis of gene expression.

[0104] SREBP-1c is a transcription factor that regulates the expression of lipid biosynthesis genes (eg, FAS, ACC, etc.) in hepatocytes, and is also a major target gene in hepatocytes as a lipid sensor for LXRα activation. Ther...

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Abstract

The present invention relates to a pharmaceutical composition and a health functional food composition for preventing and treating non-alcoholic fatty liver disease, which comprises red ginseng concentrate having enhanced Compound K as an effective ingredient by using an enzyme conversion technique.

Description

technical field [0001] The present invention relates to a pharmaceutical composition comprising compound K fortified red ginseng concentrate as an active ingredient for preventing and treating nonalcoholic fatty liver disease and a compound K fortified red ginseng concentrate for preventing and improving nonalcoholic fatty liver disease. Ginseng concentrate is a health functional food as an active ingredient. Background technique [0002] Ginseng (Panaxginseng C.A. Mayer) is a plant belonging to the genus Araliaceauginseng, and has been used for medicine in China since BC, for trade and medicine since the Three Kingdoms period, and has been widely used in various fields until now herbs or health functional food. [0003] Ginseng contains about 3% to 5% of prosaponin called ginsenoside, which is a representative physiologically active substance of ginseng and about 33 kinds of ginsenosides have been reported. Since the ginsenoside of ginseng is linked to the constituent com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61P1/16A23L33/105
CPCA23V2002/00A61K36/258A23V2200/32A23V2250/21A61K31/575A61K31/7032A23L33/105A61P1/16A61P3/06A23V2200/30A23V2250/2124A61K31/56
Inventor 白世熙徐溶基宋智慧文炳锡申兴燮申和秀
Owner CJ CHEILJEDANG CORP
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