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A method to obtain safe and effective umbilical cord mesenchymal stem cells

A technology for stem cells and umbilical cord, applied in the field of umbilical cord mesenchymal stem cells, can solve the problems of low separation efficiency, long cycle, insufficient safety, etc., and achieve the effects of improving separation efficiency, ensuring safety, and shortening culture period

Active Publication Date: 2019-01-25
FUJIAN YINFENG STEM CELL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The invention provides a method for isolating umbilical cord mesenchymal stem cells from human umbilical cord Wharton's Jelly (Wharton's Jelly) and carrying out subculture to finally obtain cell products. Problems such as low efficiency, long cycle, and insufficient security

Method used

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  • A method to obtain safe and effective umbilical cord mesenchymal stem cells
  • A method to obtain safe and effective umbilical cord mesenchymal stem cells
  • A method to obtain safe and effective umbilical cord mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1 Umbilical cord preparation:

[0049] 1) In a sterile environment, the umbilical cord is collected during the third stage of labor, and the residual blood of the umbilical cord and vein is drained and ligated; the collected umbilical cord is disinfected and placed in a storage bottle with storage liquid, and sent to the laboratory for further investigation. Prepared separately. The storage solution is 40ml of α-MEM, and gentamicin 100U / ml, penicillin 50mg / ml, and amphotericin B 5ug / ml are added. Before collection, ensure that the five tests of the pregnant woman's virus are negative and the ALT test is qualified. The transportation temperature is 4°C, and the transportation time does not exceed 24 hours.

[0050] 2) The umbilical cord bottle is wiped with alcohol and placed in a biosafety cabinet.

[0051] 3) at 150cm 2 In the cell culture dish, wash the umbilical cord 3 times with washing solution, cut off 1 cm at both ends of the umbilical cord with pointed strai...

Embodiment 2

[0078] 1 Umbilical cord preparation:

[0079] 1) In a sterile environment, the umbilical cord is collected during the third stage of labor, and the residual blood of the umbilical cord and vein is drained and ligated; the collected umbilical cord is disinfected and placed in a storage bottle with storage liquid, and sent to the laboratory for further investigation. Prepared separately. The storage solution is 40ml of α-MEM, and gentamicin 100U / ml, penicillin 50mg / ml, and amphotericin B 5ug / ml are added. Before collection, ensure that the five tests of the pregnant woman's virus are negative and the ALT test is qualified. The transportation temperature is 4°C, and the transportation time does not exceed 24 hours.

[0080] 2) The umbilical cord bottle is wiped with alcohol and placed in a biosafety cabinet.

[0081] 3) at 150cm 2 In the cell culture dish, wash the umbilical cord 3 times with washing solution, cut off 1 cm at both ends of the umbilical cord with pointed strai...

Embodiment 3

[0108] 1 Umbilical cord preparation:

[0109] 1) In a sterile environment, the umbilical cord is collected during the third stage of labor, and the residual blood of the umbilical cord and vein is drained and ligated; the collected umbilical cord is disinfected and placed in a storage bottle with storage liquid, and sent to the laboratory for further investigation. Prepared separately. The storage solution is 40ml of α-MEM, and gentamicin 100U / ml, penicillin 50mg / ml, and amphotericin B 5ug / ml are added. Before collection, ensure that the five tests of the pregnant woman's virus are negative and the ALT test is qualified. The transportation temperature is 4°C, and the transportation time does not exceed 24 hours.

[0110] 2) The umbilical cord bottle is wiped with alcohol and placed in a biosafety cabinet.

[0111] 3) at 150cm 2 In the cell culture dish, wash the umbilical cord 3 times with washing solution, cut off 1 cm at both ends of the umbilical cord with pointed strai...

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Abstract

The invention provides a method for acquiring a safe and effective umbilical cord mesenchymal stem cell. A pollution-free umbilical cord mesenchymal stem cell can be prepared by umbilical cord acquisition, umbilical cord transportation, umbilical cord preparation and methods of detecting, screening, adding antibiotics and the like in a cell culturing process, so that the safety can be guaranteed; and improved culture system and culture method are adopted in the separating and culturing processes, so that the separation efficiency is improved, the culturing cycle is shortened, and a high-purity excellent-characterization cell can be obtained. According to the method, the cost can be effectively reduced, the high-quality umbilical cord mesenchymal stem cell can be quickly acquired, injury on cells can be reduced, the culturing cycle can be shortened, pollution can be reduced, and the cell purity can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for obtaining safe and effective umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are a type of pluripotent stem cells derived from the early mesoderm and ectoderm. MSCs have functions such as immune regulation, promotion of hematopoietic recovery, ability to repair damaged or diseased tissues and organs, and multi-lineage differentiation potential, so they have broad application prospects. [0003] MSCs were first discovered in bone marrow, and now we can isolate mesenchymal stem cells from fat, synovium, bone, muscle, lung, liver, pancreas and other tissues, as well as placenta, amniotic fluid, and umbilical cord. Isolation of mesenchymal stem cells from the umbilical cord has the advantages of convenient collection, easy storage and transportation, no harm to the donor, easy separation, high purity, and no imm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2501/11C12N2501/135C12N2501/24C12N2501/25C12N2509/10
Inventor 苏爱盟肖桂清
Owner FUJIAN YINFENG STEM CELL ENG
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