Preparation method and application of biosensor built based on gold and copper-multiwalled carbon nanotube-manganese dioxide

A technology of multi-walled carbon nanotubes and biosensors, applied in instruments, scientific instruments, and electrochemical variables of materials, etc., can solve the problems of elevated serum CA125, early diagnosis of epithelial ovarian cancer, and early case elevations, etc. Achieve sensitive detection, superior electrochemical catalytic performance, and low detection limit

Active Publication Date: 2016-05-25
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

80% of patients with ovarian epithelial tumors have elevated serum CA125, but nearly half of the early cases do not increase, so it is not used alone for early diagnosis of ovarian epithelial cancer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 A preparation method of a biosensor based on gold copper-multi-walled carbon nanotubes-manganese dioxide

[0031] (1) Polish the glassy carbon electrode with alumina powder of 1.0, 0.3, and 0.05 μm in sequence, ultrasonically clean it in ultrapure water and ethanol, and dry it with nitrogen;

[0032] (2) Add 6 μL of gold copper-multi-walled carbon nanotubes-manganese dioxide aqueous solution with a concentration of 1 mg / mL dropwise on the electrode surface, and dry;

[0033] (3) Continue to drop 6 μL of CA125 antibody solution with a concentration of 5 μg / mL onto the surface of the modified electrode, incubate in a refrigerator at 4°C for 1 hour, and clean it;

[0034] (4) Block non-specific active sites with 3 μL of bovine serum albumin solution with a concentration of 5 mg / mL, incubate in a refrigerator at 4 °C for 1 h, and clean;

[0035] (5) 6 μL of a series of CA125 with a concentration of 0.0003-30 ng / mL was used for specific recognition with the anti...

Embodiment 2

[0036] Example 2 A preparation method of a biosensor based on gold copper-multi-walled carbon nanotubes-manganese dioxide

[0037] (1) Polish the glassy carbon electrode with alumina powder of 1.0, 0.3, and 0.05 μm in sequence, ultrasonically clean it in ultrapure water and ethanol, and dry it with nitrogen;

[0038] (2) Add 6 μL of gold copper-multi-walled carbon nanotubes-manganese dioxide aqueous solution with a concentration of 1.5 mg / mL dropwise on the electrode surface, and dry;

[0039] (3) Continue to drop 6 μL of CA125 antibody solution with a concentration of 10 μg / mL onto the surface of the modified electrode, incubate in a refrigerator at 4°C for 1 hour, and clean it;

[0040] (4) Block non-specific active sites with 3 μL of bovine serum albumin solution with a concentration of 10 mg / mL, incubate in a refrigerator at 4 °C for 1 h, and clean;

[0041] (5) 6 μL of a series of CA125 with a concentration of 0.0003-30 ng / mL was used for specific recognition with the ...

Embodiment 3

[0042] Example 3 A preparation method of a biosensor based on gold copper-multi-walled carbon nanotubes-manganese dioxide

[0043] (1) Polish the glassy carbon electrode with alumina powder of 1.0, 0.3, and 0.05 μm in sequence, ultrasonically clean it in ultrapure water and ethanol, and dry it with nitrogen;

[0044] (2) Add 6 μL of gold copper-multi-walled carbon nanotubes-manganese dioxide aqueous solution with a concentration of 2 mg / mL dropwise on the electrode surface, and dry;

[0045] (3) Continue to drop 6 μL of CA125 antibody solution with a concentration of 20 μg / mL onto the surface of the modified electrode, incubate in a refrigerator at 4°C for 1 hour, and clean it;

[0046] (4) Block non-specific active sites with 3 μL of bovine serum albumin solution with a concentration of 20 mg / mL, incubate in a refrigerator at 4 °C for 1 h, and clean;

[0047] (5) 6 μL of a series of CA125 with a concentration of 0.0003-30 ng / mL was used for specific recognition with the an...

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PUM

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Abstract

The invention relates to a preparation method and application of a biosensor built based on gold and copper-multiwalled carbon nanotube-manganese dioxide, and belongs to the technical field of novel function materials and biosensing detection. Based on the gold and copper-multiwalled carbon nanotube-manganese dioxide, the gold and copper-multiwalled carbon nanotube-manganese dioxide has good electrochemical catalysis capacity and electron transfer capacity to hydrogen peroxide, therefore, the sensitivity of the biosensor is apparently improved, and great significance for CA125 detection is achieved.

Description

technical field [0001] The invention discloses a preparation method and application of a biosensor based on gold copper-multi-walled carbon nanotubes-manganese dioxide. Specifically, a gold copper-multi-walled carbon nanotube-manganese dioxide with good electrochemical catalytic performance is used to prepare a sensor for detecting CA125, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] CA125 is a glycoprotein that can be bound by the monoclonal antibody OC125 detected by Bast et al. from the epithelial ovarian cancer antigen in 1983. It is derived from the body cavity epithelium during embryonic development and does not exist in normal ovarian tissue. Therefore, it is most common in epithelial ovary In the serum of patients with tumors (serous tumors), the sensitivity of the diagnosis is high, but the specificity is poor. Absent in mucinous ovarian tumors. Serum CA125 is elevated in 80% of patients wi...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/30
CPCG01N27/30G01N27/416
Inventor 魏琴胡丽华范大伟吴丹孙旭闫涛马洪敏张勇庞雪辉杜斌
Owner UNIV OF JINAN
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