Kit for simultaneously detecting eight pig disease related antibodies and use method of kit

Abstract

The invention discloses a kit for simultaneously detecting eight pig disease related antibodies against CSFV (classical swine fever virus), PPV (porcine parvovirus), a PRV (porcine pseudorabies virus), PRRSV (porcine reproductive and respiratory syndrome virus), PCV2 (porcine circovirus), PEDV (porcine epidemic diarrhea virus), TGEV (transmissible gastroenteritis virus) and SIV (swine influenza virus). The kit comprises a reaction card, an ELA reagent, a color developing agent, a stop solution, a concentrated cleaning solution and a sample diluent. The invention further provides a method for simultaneously detecting eight virus related antibodies in vitro by the aid of the kit. The kit can accurately detect a CSFV antibody, a PPV antibody, a PRV antibody, a PRRSV antibody, a PCV2 antibody, a PEDV antibody, a TGEV antibody, an SIV antibody and the like and has the advantages of high sensitivity, high specificity, good accuracy, simplicity and convenience in operation, low consumption of samples, objective judgment of results and the like.

Description

technical field [0001] The invention relates to a kit for simultaneously detecting swine disease-related antibodies, in particular to simultaneously detecting swine fever virus (CSFV), porcine parvovirus (PPV), porcine pseudorabies virus (PRV), porcine blue ear virus (PRRSV), and porcine circovirus (PCV2), porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), swine influenza virus (SIV) 8 kinds of pig disease-related antibody kits, the present invention also relates to the use method of the kit, The invention belongs to the detection field of swine disease antibodies. Background technique [0002] In recent years, with the continuous development of the breeding industry, the amount of livestock and poultry breeding has increased day by day, and intensive and large-scale farms have emerged in an endless stream. In the practice of breeding production, some large-scale pig farms only focus on immunization and vaccination for the prevention ...

AI Technical Summary

Problems solved by technology

Colloidal gold immunochromatography technology can prompt the presence of virus antibodies, but one test can only detect a single indicator, the throughput is low, and the type of virus antibodies cannot be specifically and objectively evaluated, so its specificity needs other techniques such as Western blot Secondary confirmation tests such as method, ELISA, etc., and the identification of colloidal gold immunochromatography technology has a certain degree of subjectivity, so the interpretation of the results may vary at different time points and between different observers; enzyme-linked immunosorbent assay (ELISA) has many advantages such as high maturity, high sensitivity, strong specificity, simple and fast operation method, no radioactive contamination, and the detection result eliminates the subjective judgment of thought, as well as a wide range of applications, but there is also a test that can only detect a single indicators, low throughput, high detection cost, and great limitations in the monitoring, application and promotion of the immune level of swine epidemics; immunoblotting diagnostic reagents are developed on the basis of gel electrophoresis and solid-phase immunoassay techniques. An immunobiochemical technique with the advantages of high resolution of SDS-PAGE and high specificity and sensitivity of solid-phase immunoassay

However, in the process of SDS-PAGE, the conformation of many antigenic determinants may be destroyed, and they cannot be combined with specific antibodies or non-specifically, resulting in misjudgment of results

Images