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Trichoderma asperellum spore wettable powder composition and preparation method thereof

A technology of wettable powder and Trichoderma aculeatus, applied in botany equipment and methods, chemicals for biological control, biocides, etc., can solve the problem of not being able to fully function, and the stability of field control effects cannot be guaranteed , short shelf life of biocontrol pesticide preparations, etc., to achieve the effect of long shelf life, long duration and high survival rate

Inactive Publication Date: 2016-06-08
江西正邦作物保护股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the shelf life of the biocontrol pesticides currently on the market is generally only about 3 months, which leads to the inability to guarantee the stability of the field control effect and cannot fully play a role in plant disease control.
It can be seen that the short shelf life of biocontrol pesticide preparations is the bottleneck in the production and application of biopesticides

Method used

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  • Trichoderma asperellum spore wettable powder composition and preparation method thereof
  • Trichoderma asperellum spore wettable powder composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The cultivation and screening of embodiment 1 heat-resistant Trichoderma aculeatus spore powder

[0029] 1) transfer the Trichoderma strain to PDA medium, and culture at a constant temperature of 25-28° C. to obtain the first-generation Trichoderma aculeatus spore powder F;

[0030] 2) thermally storing the first-generation Trichoderma aculeatus spore powder at a temperature of 45° C. and 54° C. for 30 days, and screening out the still-surviving first-generation Trichoderma aculeatus spore powder;

[0031] 3) Propagate the surviving first generation Trichoderma aculeatus spore powder to obtain the next generation spore powder F1, and cultivate it in batches;

[0032] 4) thermally storing the obtained Trichoderma aculeatus spore powder F1 again at temperatures of 45°C and 54°C respectively to obtain spore powder F2;

[0033] 5) After repeating 2)-4) twice, the obtained spore powder is sieved (200 mesh), and finally the Trichoderma aculeatus spore powder is obtained;

...

Embodiment 3

[0039] The screening of embodiment 3 auxiliary agent

[0040] The adjuvants with little effect on the activity of Trichoderma aculeatus spore powder were screened out. First, mix the auxiliaries after air powder with Trichoderma aculeatus evenly in a certain proportion, store them at room temperature, measure the viable spore rate of Trichoderma aculeatus for each treatment after 7 days, 30 days, 60 days, and half a year. The spore powder of Trichoderma aculeatus was used as the control.

[0041] Table 2 Screening of wetting agents

[0042]

[0043] The results showed that among wetting agents, sodium alkylbenzenesulfonate, sodium alkylsulfate and tea saponin had the least effect on the activity of Trichoderma spore powder.

[0044] Table 3 Screening of dispersants

[0045]

[0046] The results showed that among the dispersants, lignin sulfonate, naphthalene sulfonate, polycarboxylate, polynaphthalene sulfonate and anion-nonionic composite agent had the least effect o...

Embodiment 4

[0050] Implementation formula of embodiment 4 Trichoderma aculeatus spore wettable powder composition

[0051] (1) Wettable powder formulation

[0052] Recipe 1: 3.5% Trichoderma aculeatus spore powder

[0053] Trichoderma aculeatus spore powder 3.5%, anion-nonionic compound (GY-D04) 3%, alkylbenzenesulfonate (8906) 3%, anion-nonionic compound (YP) 5%, naphthalenesulfonic acid Salt (NNO) 5%, corn starch 10%, white carbon black 5%, talcum powder 15%, light calcium 15%, washed kaolin supplement. The original drug, dispersant, wetting agent, stabilizer and carrier are fully mixed first, and ultrafine jet crushing is carried out to form a finished product.

[0054] Recipe 2: 10% Trichoderma aculeatus spore powder

[0055] Trichoderma aculeatus spore powder 10%, anion-nonionic compound (GY-D10) 4%, polycarboxylate (T36) 3%, lignosulfonate (CA-SA) 5%, alkylbenzene sulfonate Hydroxide (EFW) 7%, corn starch 10%, white carbon black 5%, Yuanming powder 15%, light calcium 10%, washed...

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Abstract

The invention relates to the field of biopesticide, and in particular to a Trichoderma asperellum spore wettable powder composition and a preparation method thereof. The wettable powder composition consists of the following components: 10-55% of Trichoderma asperellum spore powder, 2-15% of a wetting agent, 2-8% of a dispersant, 6-10% of a stabilizer and the balance of a carrier. The Trichoderma asperellum spore powder is subjected to heat storage culture and screening, and is cultured to at least three generations and bred. The spore powder has a higher survival rate at high temperature and under heat storage, so that the product has a long shelf life, can be stored for a long time at a predetermined temperature, and has a long persistence period under high temperature application conditions.

Description

technical field [0001] The invention relates to the field of biological pesticides, in particular to a Trichoderma aculeatus spore wettable powder composition and a preparation method thereof. Background technique [0002] The use of chemical pesticides for the prevention and control of soil-borne diseases is large, and excessive use of pesticides leads to serious residues exceeding the standard, which has a serious negative impact on the environment. With people's emphasis on environmental protection and sustainable development of agriculture, more environmentally friendly biological control methods Pesticides (hereinafter referred to as biocontrol pesticides) are getting more and more attention, and the development of biocontrol pesticides is increasingly supported by national policies. However, the shelf life of the biocontrol pesticides currently on the market is generally only about 3 months, which leads to the inability to guarantee the stability of the control effect ...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/14A01P3/00
CPCA01N25/14A01N63/30A01N2300/00
Inventor 黄婷魏方林徐沛东肖永良
Owner 江西正邦作物保护股份有限公司
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