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Traditional Chinese medicine composition formed by ginkgo biloba extract and application thereof to preparation of Shuxuening parenteral solution

An extract and composition technology, applied in the application field of preparing Shuxuening injection, can solve the problems that the chemical composition is not fully confirmed, the active ingredient, the mechanism of action, pharmacokinetics and adverse reactions are not fully explored. , to achieve the effect of benefiting the quality of medicines, reducing adverse reactions, and facilitating separation and operation

Inactive Publication Date: 2016-06-08
北京华润高科天然药物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Restricted by technological development, the chemical components in Ginkgo biloba extract have not been fully confirmed, and the active ingredients, mechanism of action, pharmacokinetics and adverse reactions have not been fully explored.

Method used

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  • Traditional Chinese medicine composition formed by ginkgo biloba extract and application thereof to preparation of Shuxuening parenteral solution
  • Traditional Chinese medicine composition formed by ginkgo biloba extract and application thereof to preparation of Shuxuening parenteral solution
  • Traditional Chinese medicine composition formed by ginkgo biloba extract and application thereof to preparation of Shuxuening parenteral solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of quercetin-3-O-glucoside

[0047] Weigh 10 g of Ginkgo biloba extract, dissolve it in 50 mL of 40% ethanol-water solution to obtain a Ginkgo biloba extract solution with a concentration of 200 mg / mL, pass through a 0.45 μm microporous membrane, and perform one-dimensional liquid chromatography separation. One-dimensional liquid chromatography adopts silica gel as the matrix of hydrophilic chromatographic filler 50 × 250mm, 10 μm (Huapu New Innovation Technology Co., Ltd.), and the mobile phase adopts methanol containing 0.1% volume concentration of formic acid as the organic phase, containing 0.1% volume The water with formic acid concentration is the water phase, and the gradient elution method: the concentration of the organic phase decreases from 95% to 90% in step gradient in 0-15min. Use DAD ultraviolet detector 360nm to select the absorption wavelength, the preparation temperature is room temperature, the injection volume is 500μL / needle, the mobile ...

Embodiment 2

[0068] Preparation of quercetin-3-O-glucoside

[0069] Weigh 1 g of Ginkgo biloba extract, dissolve it in 100 mL of ethanol-water solution with a volume concentration of 80%, and obtain a Ginkgo biloba extract solution with a concentration of 10 mg / mL, pass it through a 0.45 μm microporous membrane, and perform one-dimensional liquid chromatography. One-dimensional liquid chromatography adopts silica gel-based hydrophilic chromatographic packing (50×250mm, 10μm, Huapu Innovation Technology Co., Ltd.), the mobile phase uses ethanol as the organic phase, water as the aqueous phase, and the concentration of the organic phase is 90 % isocratic, using a DAD ultraviolet detector at 360nm to select the absorption wavelength, the preparation temperature is 40°C, the injection volume is 200μL / needle, the flow rate of the mobile phase is 60mL / min, the components and the remaining components are collected for 15-20 minutes, and the The fractions collected for 15 to 20 minutes were concen...

Embodiment 3

[0078] Preparation of quercetin-3-O-glucoside

[0079] Weigh 100g of Ginkgo biloba extract, dissolve it in 200mL of 50% ethanol-water solution to obtain a Ginkgo biloba extract solution with a concentration of 500mg / mL, pass it through a 0.45μm microporous membrane, and prepare it by one-dimensional liquid chromatography. One-dimensional liquid chromatography adopts a hydrophilic chromatographic packing (50×250mm, 10 μ, Huapu Innovation Technology Co., Ltd.) based on silica gel, and the mobile phase adopts ethanol (containing 0.1% formic acid) as the organic phase, water (containing 0.1% % formic acid) is the aqueous phase, and is eluted by 95% organic equivalence. The DAD ultraviolet detector was used to select the absorption wavelength at 360nm, the preparation temperature was 30°C, the injection volume was 3000 μL / needle, the flow rate of the mobile phase was 120mL / min, the components and the remaining components were collected for 15-20 minutes, and the collected 15 Fract...

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Abstract

The invention discloses a traditional Chinese medicine composition formed by ginkgo biloba extract. The traditional Chinese medicine composition is prepared from, by mass, 24-40% of gingko total flavone, 6-16% of bilobalide, and ginkgolic acid with the amount smaller than 5 ppm. Preferably, the traditional Chinese medicine composition is further prepared from 1-1.68% of quercetin-3-O-glucoside and 2.11-3.53% of quercetin-3-O-2'',6''-dirhamnose glucoside. Preferably, the traditional Chinese medicine composition is further prepared from 1.4-3% of bilobalide A, 0.9-1.8% of bilobalide B and 1.2-1.3% of bilobalide C. Quercetin-3-O-glucoside and quercetin-3-O-2'',6''-dirhamnose glucoside having an important influence on the medicine are definite, the content of quercetin-3-O-glucoside and the content of quercetin-3-O-2'',6''-dirhamnose glucoside are accurately defined as 1-1.68% and 2.11-3.53%, furthermore, biobalide A is clearly defined as 1.4-3%, biobalide B is clearly defined as 0.9-1.8% and biobalide B is clearly defined as 1.2-1.3%. By making the content of the effective components in the ginkgo biloba extract to be accurate and further affirming the effective components, efficacy of the ginkgo biloba extract is improved.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition formed from ginkgo biloba extract, and an application of the composition in preparing Shuxuening injection. Background technique [0002] Ginkgo biloba is the dried leaf of Ginkgo biloba plant, Ginkgo biloba extract is the enriched product of active substances extracted from Ginkgo biloba through modern extraction technology, which can be used for Alzheimer's disease, depression, diabetes, neurological diseases, impotence , memory impairment, peripheral vascular disease, intermittent claudication, brain turning tinnitus and other diseases. Its main active ingredients are flavonoids and terpenes. Flavonoids include monoflavones, flavonol glycosides, acetylated flavonol glycosides, biflavones, flavan-3-ols, and proanthocyanidins. Terpenoid ginkgolides include ginkgolides A, B, C, J, M and bilobalide. [0003] At present, the...

Claims

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Application Information

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IPC IPC(8): A61K36/16A61K9/08A61K31/365A61K31/7048A61P9/10C07H17/07C07H1/08
CPCA61K36/16A61K9/0019A61K9/08A61K31/365A61K31/7048A61K2236/333A61K2236/55C07H1/08C07H17/07A61K2300/00
Inventor 王海涛王秋平杜秀琴
Owner 北京华润高科天然药物有限公司
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