Pear psylla control medicament and application thereof in control of pear psylla

A pear psyllid and medicament technology, applied in the field of biological pesticides, can solve the problems of rampant pests in pear orchards, killing natural enemies in orchards, etc., achieving significant lethal effects, protecting the environment, and low production costs

Inactive Publication Date: 2016-06-15
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this period is also the peak period of natural enemy insects in pear orchards. The application of chemical pesticides will kill a large number of natural enemies in orcha

Method used

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  • Pear psylla control medicament and application thereof in control of pear psylla
  • Pear psylla control medicament and application thereof in control of pear psylla

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The preparation method of embodiment 1 Nepeta essential oil

[0039] In the present invention, a method for extracting Nepeta essential oil is provided, but the essential oil of Nepeta nepeta for realizing the purpose of the present invention is not limited to the extraction method.

[0040] Dry 500-1000g Nepeta plant, crush it to 40-120 mesh, weigh the crushed Nepeta in a 3000ml round bottom flask, add 1000-2000ml water, soak for 2-4h, then distill by continuous distillation After 3-4 hours, the obtained oil-water mixture is extracted with n-hexane. After dehydration, the extracted product is evaporated and concentrated under reduced pressure in a water bath at 40-45°C with a rotary evaporator, and n-hexane is recovered. The obtained residual oily liquid is soil Mustard essential oil.

Embodiment 2

[0041] Indoor toxicity assay of embodiment 2 Nepeta essential oil

[0042] In this experiment, the micro-drop method was used to determine the contact activity of nepeta essential oil on pear psyllids, and the steps were as follows:

[0043] (1) Take a 12-well cell culture plate, coat the inner wall of the culture plate with polytetrafluoroethylene, and dry it with a hair dryer; mix water and agar at a ratio of 100:2, heat and mix in a microwave oven, and pour into the 12-well cell In the culture plate (about 2-3ml per well), cool until solidified.

[0044] (2) Fresh pear leaves are made into circular blades with a puncher with a diameter of 19 mm, and placed on a culture plate poured with agar upside down.

[0045] (3) Using acetone as a solvent, dilute the Nepeta essential oil to 0.13%, 0.20%, 0.30%, 0.44%, 0.67%, 1.00%.

[0046] (4) Select healthy adults of the same size on a petri dish, spot 0.5 μl of diluted medicinal solution on the pronotum of the adults, and insert the...

Embodiment 3

[0050] Embodiment 3 Nepeta essential oil field control technology of pear psyllid

[0051] Mix the total volatile oil of Nepeta nepeta and detergent at 1:1, dilute the mixture 2500 times with water as a solvent, and spray the branches of the treatment group with a special pesticide sprayer for orchards (spray on the front and back of pear leaves, spray To just have dripping water as the criterion), spray the branches of the control group with the solvent that does not contain Nepeta essential oil, investigate the surviving number of adults of Psyllium pear on the branches after spraying for 72h and 192h, and calculate the mortality of Psyllium pear adults.

[0052] Field experiments showed that Nepeta essential oil had a good control effect on Psyllium adults. After spraying for 72 hours, the control effect of Nepeta essential oil on adults of Psyllium was 41%. The control effect is 35% ( figure 1 ).

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Abstract

The present invention relates to a pear psylla control medicament and application thereof in control of pear psylla, and belongs to the technical field of biotechnology pesticides. The pear psylla control medicament has significant lethal effects on pear psylla, compared with traditional pear psylla control methods, pharmaceutical ingredients of the pear psylla control medicament are derived from chenopodium ambrosioides, after use, the pear psylla control medicament is free of drug residue, can protect the environment, does not kill orchard pear psylla natural enemies, maintains ecological balance, is low in production cost, and facilitates extensive use in orchard pear psylla control. Research results provide a basis for further research and promotion of biological agents of chenopodium ambrosioides, and have far-reaching significance on long-term development of the ecological environment, human health and comprehensive management of diseases.

Description

technical field [0001] The invention relates to a medicament for preventing and treating psyllid and its application in preventing and treating psyllium, belonging to the technical field of biological pesticides. Background technique [0002] The Chinese pear psyllid Psyllachinensis YangetLi, belonging to the family Psyllidae of the order Homoptera, is also known as the pear psyllid. It is widely distributed in China and is one of the most important pests in pear orchards. In recent years, this pest has seriously occurred in pear orchards in North China, Northeast China and even Jiangnan. , becoming one of the key factors affecting pome fruit production. [0003] The current methods of comprehensive control of pear psyllids include: [0004] (1) Agricultural control: Clean the orchard in early spring and late autumn, scrape the bark and apply base fertilizer, collect fallen leaves and weeds, and bury them deeply together with the fertilizer. [0005] (2) Artificial physica...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P7/04
Inventor 刘志龙刘昕超田宝良刘奇志石旺鹏
Owner CHINA AGRI UNIV
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